| Abstract | 第1-7页 |
| 中文摘要 | 第7-9页 |
| Abbreviations | 第9-11页 |
| Chapter Ⅰ A brief review Revolution in genome editing:CRISPR/Cas9 system and its applications | 第11-69页 |
| ·Gene targeting tools | 第12-17页 |
| ·Homologous recombination | 第12-14页 |
| ·Programmable nucleases | 第14-17页 |
| ·A brief introduction of CRISPR/Cas system | 第17-23页 |
| ·Evolution of CRISPR system | 第18-19页 |
| ·Components and mechanism of CRISPR/Cas system | 第19-20页 |
| ·Classification of CRISPR/Cas system | 第20-22页 |
| ·Type II CRISPR/Cas system | 第22-23页 |
| ·CRISPR/Cas9 as a genome editing tool | 第23-42页 |
| ·Structure of SpCas9 | 第23-25页 |
| ·CRISPR/Cas9 in gene targeting | 第25-28页 |
| ·Application of Cas9 nickases | 第28-29页 |
| ·Application of CRISPR/Cas9 in high-throughput screening | 第29-31页 |
| ·Application of CRISPR/Cas9 in gene and cell therapy | 第31-33页 |
| ·CRISPR/Cas9 gene drives in species | 第33-34页 |
| ·Application of CRISPR/dCas9 | 第34-37页 |
| ·Other representative progresses of CRISPR/Cas9 | 第37-39页 |
| ·Strategies for improving specificity of CRISPR/Cas9 | 第39-41页 |
| ·Comparison of CRISPR/Cas9 with ZFN and TALEN | 第41-42页 |
| ·Problems and perspectives | 第42-44页 |
| References | 第44-69页 |
| Chapter Ⅱ Efficient in vitro and in vivo cleavage of large DNA fragments by CRISPR/Cas9 | 第69-129页 |
| Introduction | 第71-73页 |
| Materials and Methods | 第73-81页 |
| Results | 第81-121页 |
| ·CRISPR/Cas9 system for cleaving DNA in vitro | 第81-91页 |
| ·Deletion exogenous fragments in human cells by CRISPR/Cas9 system | 第91-97页 |
| ·Deletion endogenous fragments in mouse embryonic stem cells by CRISPR/Cas9 system | 第97-106页 |
| ·Efficient one-step knockout of lncRNA, Rian, via CRISPR/ Cas9-mediated fragment deletion | 第106-121页 |
| Discussions | 第121-123页 |
| References | 第123-129页 |
| Acknowledgements | 第129-130页 |
| Original publication | 第130-132页 |
