| Abstract in Chinese | 第1-14页 |
| Abstract in English | 第14-22页 |
| Part One: Research on Catecholamine Hormone's Effect on Expression of the Peripheral Cellular Inflammatory Factor | 第22-95页 |
| Introduction | 第22-24页 |
| 1 Materials | 第24-25页 |
| 1 1 Main reagents | 第24-25页 |
| ·Instrumenttations | 第25页 |
| 2 Empirical Method | 第25-47页 |
| ·Cell culture | 第26页 |
| ·Extraction of total RNA and purification of mRNA | 第26-27页 |
| ·Quantitation and integrality analysis on RNA sample | 第27-28页 |
| ·Suppression subtractive hybridization (SSH) | 第28-37页 |
| ·PCR analysis of subtraction efficiency | 第37-38页 |
| ·Construct differential expression gene of subtractive cDNA Lib | 第38-41页 |
| ·Screening subtractive cDNA Lib by reverse dot blot hybridization | 第41-44页 |
| ·DNA sequencing and analysis of homology | 第44页 |
| ·Rverse transcription-polymerase chain reaction | 第44-46页 |
| ·Detection of IL-6 protein expression by ELISA | 第46-47页 |
| ·Statistical analyses | 第47页 |
| 3 Results | 第47-83页 |
| ·Abstraction of total RNA and mRNA | 第47-48页 |
| ·Synthesis of ds cDNA and the result of Rsal digestion | 第48-49页 |
| ·Results of the ligation efficiency analysis | 第49-50页 |
| ·Two series of PCR on differential hybridization product | 第50页 |
| ·Results of subtractive hybridization efficiency analysis | 第50-51页 |
| ·Construction of subtractive cDNA Lib | 第51-52页 |
| ·Propagation of positive colonies | 第52页 |
| ·Bolting of subtractive cDNA Lib by PCR | 第52页 |
| ·The result of reverse dot blot hybridization | 第52-53页 |
| ·DNA sequencing and homology analytic results of positive colonies | 第53-54页 |
| ·The effect of adrenalin on the expression of inflammatory factors of cultured cells | 第54-65页 |
| ·The effect of noradrenalin exposed to cultured cells on the expression of inflammatory factors | 第65-75页 |
| ·The effect of cortisol exposed to cultured cells on the expression of inflammatory factors | 第75-83页 |
| 4 Discussion | 第83-90页 |
| 5 Conclusion | 第90-91页 |
| 6 References | 第91-95页 |
| Part Two: Relationship of APOE Gene Polymorphism with Subclasses of Plasma High Density Lipoprotein in Hyperlipidemia | 第95-107页 |
| Introduction | 第95-96页 |
| 1 Subjects and methods | 第96-99页 |
| ·Subjects | 第96-97页 |
| ·Specimens | 第97页 |
| ·Genetic analyses | 第97-98页 |
| ·Plasma lipid and apolipoprotein analyses | 第98页 |
| ·HDL subclasses analyses | 第98-99页 |
| ·Statistical analyses | 第99页 |
| 2 Results | 第99-102页 |
| ·Frequency distributions of APOE genotypes and alleles in hyperlipidemic and control groups | 第99-100页 |
| ·Concentrations of plasma lipids, lipoproteins, apolipoproteins and relative APOAI percentage contents of plasma HDL subclasses | 第100-101页 |
| ·Lipid parameters and relative APOAI percentage contents of plasma HDL subclasses with different APOE genotypes | 第101-102页 |
| 3 Discussion | 第102-105页 |
| 4 Conclusion | 第105页 |
| 5 References | 第105-107页 |
| Part Three: Relationship between Plasma HDL Subclasses Distribution and Lipoprotein Lipase Gene HindⅢ Polymorphism in Hyperlipidemia | 第107-122页 |
| Introduction | 第107-108页 |
| 1 Subjects and methods | 第108-111页 |
| ·Subjects | 第108-109页 |
| ·Specimens | 第109页 |
| ·Plasma lipid and apolipoprotein analyses | 第109-110页 |
| ·HDL subclasses analyses | 第110页 |
| ·Genetic analyses | 第110-111页 |
| ·Statistical analyses | 第111页 |
| 2 Results | 第111-114页 |
| ·Frequency distributions of LPL genotypes and alleles in the hyperlipidemic and control group | 第111-112页 |
| ·Demographic characteristics, lipid parameters and relative apoA-I contents of plasma HDL subclasses | 第112-113页 |
| ·Lipid parameters and relative apoA-I contents of plasma HDL subclasses with different LPL genotypes | 第113-114页 |
| 3 Discussion | 第114-116页 |
| 4 Conclusion | 第116-117页 |
| 5 References | 第117-122页 |
| Review | 第122-134页 |
| Brief introduction of technological research findings during study | 第134-135页 |
| The statement of thesis' originality | 第135-136页 |
| Acknowledgements | 第136页 |
