| ABSTRACT | 第1-6页 |
| 中文摘要 | 第6-10页 |
| 1 INTRODUCTION | 第10-14页 |
| 2 LITERATURE REVIEW | 第14-39页 |
| ·Role of neuroinflammation for Parkinson’s disease | 第14-31页 |
| ·Parkinson’s disease | 第14-21页 |
| ·Neuroinflammation and pathological features of specific disease | 第21-31页 |
| ·Anti-inflammatory action of natural compound | 第31-39页 |
| ·Flavonoids as active ingredients to inhibit neuroinflammation | 第32-33页 |
| ·Neuroprotective effects of ginsenosides | 第33-39页 |
| 3 MATERIALS AND METHODS | 第39-48页 |
| ·Materials and apparatus | 第39页 |
| ·Methods | 第39-47页 |
| ·Preparation of rat primary neuronal-glial cultures | 第39-42页 |
| ·Treatment with LPS in mice mesencephalic dissociated cultures | 第42页 |
| ·Identification of TH+ neurons | 第42-43页 |
| ·Treatment with LPS and ginsenoside Rd | 第43-44页 |
| ·Nitrite assay | 第44-45页 |
| ·PGE_2 Assay | 第45-46页 |
| ·LDH activity assay | 第46页 |
| ·Measurement of glucose | 第46-47页 |
| ·Statistical analysis | 第47-48页 |
| 4 RESULTS | 第48-68页 |
| ·Growth of the cell culture | 第48页 |
| ·LPS had dose dependent toxicity to TH+ neurons | 第48-52页 |
| ·Effect of LPS on astrocytes cell cultures | 第52页 |
| ·LPS equally affects others neuronal populations | 第52-53页 |
| ·LPS increased NO formation | 第53-55页 |
| ·Measurement of LDH-release from cells supernatants | 第55-56页 |
| ·Different sources of LPS showed different neurotoxicity | 第56-57页 |
| ·Ginsenoside Rd protected dopaminergic neurons against LPS-neurotoxicity | 第57-59页 |
| ·Effect of ginsenoside Rd on LPS-induced NeuN+ cytotoxicity | 第59-61页 |
| ·Inhibition of LPS-induced NO formation by ginsenoside Rd | 第61-62页 |
| ·Effect of ginsenoside Rd on LPS-induced PGE2 production | 第62-63页 |
| ·Effect of indomethacin on LPS-induced TH+ neurontoxin | 第63-64页 |
| ·L-DOPA partially protected against cytotoxicity induced by LPS | 第64-65页 |
| ·L-DOPA partially inhibited NO formation by LPS | 第65-66页 |
| ·Effect of bromocriptine on the TH+ cells against LPS exposure | 第66-67页 |
| ·Effect of bromocriptine on NO generation in LPS treated cultures | 第67-68页 |
| 5 DISCUSSION | 第68-80页 |
| ·Mesencephalic neuron-glial cultures is used for models of Parkinson’s disease | 第68页 |
| ·LPS is neurotoxic to dopaminergic neurons in mesencephalic culture | 第68-69页 |
| ·Glial over-activation and overproduction of NO and PGE2 are deleterious to neurons | 第69-71页 |
| ·LPS-induced glial activation and dopaminergic neurodegeneration | 第71-75页 |
| ·Overproduction of proinflammatory factors are deleterious to neurons | 第75-77页 |
| ·Anti-inflammatory drugs | 第77-78页 |
| ·Ginsenoside Rd protected dopaminergic neurons from LPS | 第78-80页 |
| 6 CONCLUSION | 第80-82页 |
| 7 REFERENCES | 第82-96页 |
| 8 ABBREVIATIONS | 第96-98页 |
| 9 ACKNOWLEDGEMENTS | 第98-99页 |
| 10 CURRICULUM VITAE | 第99-101页 |
| 附：试验内容中文缩写 | 第101-110页 |
