| Abstract | 第1-7页 |
| 中文摘要 | 第7-9页 |
| Abbreviations | 第9-10页 |
| Review:Cofilin, Rho GTPases and border cell migration | 第10-53页 |
| ·Biology of Cofilin | 第11-23页 |
| ·Biology of Rho GTPases | 第23-33页 |
| ·Border cell migration | 第33-42页 |
| References | 第42-53页 |
| Chapter 1:Genetic dissection of cofilin pathway in Drosophilaborder cell migration | 第53-71页 |
| Introduction | 第54-55页 |
| Materials and methods | 第55-58页 |
| Results | 第58-67页 |
| ·Cofilin is required for lamellipodial protrusion of border cells | 第58-60页 |
| ·Cofilin may function downstream of Rac during border cell migration | 第60-64页 |
| ·Rac may act through PAK and LIMK to regulate border cell migration | 第64-65页 |
| ·Rho but not Cdc42 signaling moderately promotes border cell migration through cofilin | 第65-67页 |
| Discussions | 第67-69页 |
| References | 第69-71页 |
| Chapter 2:LEGI type chemotaxis of border cells | 第71-107页 |
| Introduction | 第72-74页 |
| Materials and methods | 第74-77页 |
| Results | 第77-97页 |
| ·Cofilin distributes asymmetrically in border cells, while phosphorylated cofilin distributes uniformly | 第77-85页 |
| ·Antibodies' specificity test | 第77-82页 |
| ·Localization of cofilin in border cells | 第82-85页 |
| ·Rac is required for global and robust phosphorylation of cofilin but not asymmetry of cofilin during migration | 第85-86页 |
| ·Rho but not Cdc42 also contributes to cofilin phosphorylation albeit at a much less extent than Rac signaling | 第86-88页 |
| ·PVR activity is required for cofilin's asymmetry but not its phosphorylation | 第88-91页 |
| ·Testing the LEGI model in border cell migration | 第91-97页 |
| ·Alternations in Global Inhibition | 第92-94页 |
| ·Alternations in Local Excitation | 第94-97页 |
| Discussions | 第97-104页 |
| References | 第104-107页 |
| Acknowledgments | 第107-108页 |
| Publication | 第108-110页 |
