| List of Figures | 第10-12页 |
| Abbreviations | 第12-14页 |
| Abstract in Chinese | 第14-16页 |
| Abstract | 第16-18页 |
| Introduct | 第19-28页 |
| 1. Chronic hepatic injury | 第19-21页 |
| 2. Hepatic stellate cells in hepatic fibrosis | 第21-22页 |
| 3. TLR4 signaling pathways in liver fibrosis | 第22-24页 |
| 4. LKB1-AMPK signaling pathways in hepatic injury | 第24-25页 |
| 5. Traditional Chinese medicine in liver disease | 第25-28页 |
| Chapter 1.Thymoquinone attenuates liver fibrosis via TLR4 and LKB1-AMPKsignaling pathways | 第28-60页 |
| Abstract | 第29-31页 |
| 1.1 Introduction | 第31-34页 |
| 1.2 Materials and Methods | 第34-39页 |
| 1.2.1 Materials | 第34页 |
| 1.2.2 In vitro study | 第34-35页 |
| 1.2.2.1 Cell cultures | 第34-35页 |
| 1.2.2.2 Measurement of cell viability by MTT assay | 第35页 |
| 1.2.3 In vivo study | 第35-37页 |
| 1.2.3.1 Animals and treatments | 第35-36页 |
| 1.2.3.2 Serum biochemical parameters assay | 第36页 |
| 1.2.3.3 Histopathological analysis | 第36页 |
| 1.2.3.4 Immunohistochemistry examination | 第36页 |
| 1.2.3.5 Reverse Transcription Polymerase Chain Reaction(RT-PCR) | 第36-37页 |
| 1.2.4 Western blot analysis | 第37-38页 |
| 1.2.5 Statistical analysis | 第38-39页 |
| 1.3 Results | 第39-54页 |
| 1.3.1 In vitro results | 第39-45页 |
| 1.3.1.1 Effects of TQ on the Viability | 第39-40页 |
| 1.3.1.2 Effects of TQ on protein expression of CD14 and TLR4 | 第40-41页 |
| 1.3.1.3 Effects of TQ on HSCs apoptosis | 第41-42页 |
| 1.3.1.4 Effects of TQ on protein expression of collagen-I and α-SMA | 第42-44页 |
| 1.3.1.5 Effects of TQ on LPS-induced protein expression of PI3K/Akt phosphorylation | 第44-45页 |
| 1.3.2 In vivo results | 第45-54页 |
| 1.3.2.1 Effects of TQ on ALT and AST activities | 第45-46页 |
| 1.3.2.2 Histopathological and immunohistochemical changes in mice livers after TAA treatment | 第46-47页 |
| 1.3.2.3 Effects of TQ on the expression of collagen-I,α-SMA and TIMP-1 | 第47-49页 |
| 1.3.2.4 Effects of TQ on the expression of TLR4 | 第49-50页 |
| 1.3.2.5 Effects of TQ on TAA treated protein expression of PI3K phosphorylation | 第50-51页 |
| 1.3.2.6 Effects of TQ on the activation of AMPK signaling pathways in TAA induced liver fibrosis | 第51-52页 |
| 1.3.2.7 Effects of TQ on proinflammatory cytokines levels | 第52-54页 |
| 1.4 Discussion | 第54-60页 |
| Chapter 2.Betulin alleviated ethanol-induced alcoholic liver steatosis throughLKB1-AMPK signaling pathways | 第60-92页 |
| Abstract | 第61-63页 |
| 2.1 Introduction | 第63-66页 |
| 2.2 Materials and Methods | 第66-71页 |
| 2.2.1 Materials | 第66页 |
| 2.2.2 In vitro study | 第66-67页 |
| 2.2.2.1 Cell cultures | 第66-67页 |
| 2.2.2.2 Measurement of cell viability by MTT assay | 第67页 |
| 2.2.3 In vivo study | 第67-69页 |
| 2.2.3.1 Animals | 第67页 |
| 2.2.3.2 Chronic-binge ethanol feeding model | 第67-68页 |
| 2.2.3.3 Serum biochemical parameters and triglyceride assay | 第68页 |
| 2.2.3.4 Histopathological analysis | 第68页 |
| 2.2.3.5 Immunohistochemistry examination | 第68-69页 |
| 2.2.3.6 Immunofluorescence imaging assay | 第69页 |
| 2.2.4 Western blot analysis | 第69-70页 |
| 2.2.5 Statistical analysis | 第70-71页 |
| 2.3 | 第71-87页 |
| 2.3.1 | 第71-78页 |
| 2.3.1.3 AMPK signaling is implicated in the supp ression of ethanol-induced SREBP-1 expression by BT in LX-2 cells | 第71-72页 |
| 2.3.1.4 LKB1 signaling is implicated in the suppression of ethanol-induced SREBP-1 expression by BT in LX-2 cells | 第72-74页 |
| 2.3.1.5 Effects of BT on ethanol-induced protein expression of LKB1-AMPK phosphorylation | 第74-75页 |
| 2.3.1.6 Effect of BT on ethanol-induced SIRT1 expression | 第75-76页 |
| 2.3.1.7 Effects of BT on ethanol-induced collagen-I and α-SMA levels in LX-2 cells | 第76-78页 |
| 2.3.2 In vivo results | 第78-87页 |
| 2.3.2.1 Effects of BT on liver weight induced by chronic-binge ethanol | 第78-79页 |
| 2.3.2.2 Effects of BT on serum biochemical parameters and triglyceride assay | 第79-80页 |
| 2.3.2.3 Effects of BT on histopathological and Immunohistochemical changes induced by chronic-binge ethanol in mice | 第80-82页 |
| 2.3.2.4 Effect of BT on ch ronic-binge ethanol induced SREBP-1 expression | 第82-83页 |
| 2.3.2.5 Effects of BT on chronic-binge ethanol induced protein expression of LKB1-AMPK phosphorylation | 第83-84页 |
| 2.3.2.6 Effect of BT on chronic-binge ethanol induced SIRT1 expression | 第84-87页 |
| 2.4 Discussion | 第87-92页 |
| Conclusions | 第92-93页 |
| References | 第93-107页 |
| Publications | 第107-109页 |
| 致谢 | 第109页 |
