| 摘要 | 第4-7页 |
| Abstract | 第7-8页 |
| Content | 第9-11页 |
| Chapter 1 Introductions | 第11-33页 |
| 1.1 Acid mine drainage: origin and chemistry | 第11-15页 |
| 1.1.1 The origin of acid mine drainage | 第11-12页 |
| 1.1.2 The chemistry of acid mine drainage | 第12-14页 |
| 1.1.3 The acid mine drainage and the microbe | 第14-15页 |
| 1.2 The prevention and treatment of acid mine drainage | 第15-20页 |
| 1.2.1 The damage of the acid mine drainage | 第15页 |
| 1.2.2 The prevention of acid mine drainage | 第15-16页 |
| 1.2.3 The treatment of acid mine drainage | 第16-20页 |
| 1.3 The significance of the research on acid mine drainage | 第20-21页 |
| 1.4 The microbe in acid mine drainage | 第21-26页 |
| 1.4.1 Bacteria | 第22-25页 |
| 1.4.2 Archaea | 第25-26页 |
| 1.5 The diversity of acid mine drainage | 第26-28页 |
| 1.6 The research methods of the microbial composition in acid mine drainage | 第28-33页 |
| 1.6.1 Cultivating-based method | 第28-29页 |
| 1.6.2 Method of determining the sequences of 16S rRNA genes | 第29页 |
| 1.6.3 Fluorescent in situ hybridization (FISH) | 第29-30页 |
| 1.6.4 Microarray | 第30-33页 |
| Chapter 2 Site description and sample collection | 第33-35页 |
| 2.1 Site description | 第33-34页 |
| 2.2 Sample collection | 第34-35页 |
| Chapter 3 Materials and Methods | 第35-41页 |
| 3.1 DNA extraction and Purification | 第35-36页 |
| 3.2 PCR and fractionation of 16S rRNA genes | 第36-37页 |
| 3.3 Ligation and Transformation | 第37-38页 |
| 3.4 Reamplifying of clones and RFLP analysis | 第38-39页 |
| 3.5 Sequencing and Phylogenetic analysis | 第39页 |
| 3.6 Statistical methods | 第39页 |
| 3.7 Nucleotide sequence accession numbers | 第39-41页 |
| Chapter 4 Microbial community composition of tls2 | 第41-49页 |
| 4.1 Site biogeochemical properties of tls2 | 第41页 |
| 4.2 RFLP analysis of 16S rDNA clone libraries | 第41-43页 |
| 4.3 Sequence data and phylogenetic analysis of bacterial and archaeal sequences | 第43-45页 |
| 4.4 Discussion | 第45-49页 |
| 4.4.1 The bacterial composition | 第45-46页 |
| 4.4.2 The archaeal composition | 第46-49页 |
| Chapter 5 Bacterial diversity between AMD and sediment at tls2 | 第49-55页 |
| 5.1 The bacterial composition in sediment at site tls2 | 第49-52页 |
| 5.1.1 RFLP analysis of bacterial 16S rDNA clone libraries | 第49-50页 |
| 5.1.2 Sequence data and phylogenetic analysis of sediment bacterial sequences | 第50-52页 |
| 5.2 Discussion | 第52-55页 |
| 5.2.1 The microbial composition in the sediment at tls2 | 第52-53页 |
| 5.2.2 The diversity between the AMD and sediment at tls2 | 第53-55页 |
| Chapter 6 Microbial diversity of the AMD in the three sites | 第55-67页 |
| 6.1 Site biogeochemical properties | 第55页 |
| 6.2 The bacterial diversity of the AMD among the sites | 第55-63页 |
| 6.2.1 RFLP analysis of 16S rDNA clone libraries | 第55-57页 |
| 6.2.2 Phylogenetic analysis | 第57-60页 |
| 6.2.3 PCA anlysis of the three sites | 第60-63页 |
| 6.3 Discussion | 第63-67页 |
| 6.3.1 The main diversity of the microbial composition among the three AMDs | 第63-64页 |
| 6.3.2 The diversity of A. ferrooxidans and Leptospirillum between tls1 and tls2 | 第64-65页 |
| 6.3.3 Acidiphilium, Ferrimicrobium and Acidobacteria in the AMD of three sites | 第65-66页 |
| 6.3.4 The relationship between the microbial composition structures and the site biogeochemical properties | 第66-67页 |
| Chapter 7 Conclusions | 第67-69页 |
| Reference | 第69-83页 |
| Acknowledgements | 第83-85页 |
| Main achievements | 第85页 |
