| Abstract | 第5页 |
| 中文摘要 | 第7-9页 |
| Abbreviations/Acoronyms | 第9-11页 |
| Chapter Ⅰ: A Brief Review:Spermatogenesis and RHAU, a G4 DNAhelicase | 第11-54页 |
| 1.1 Overview of spermatogenesis | 第12-27页 |
| 1.1.1 Morphology and cell biology of male germ cells | 第12-25页 |
| 1.1.1.1 Morphology of male germ cells | 第12-14页 |
| 1.1.1.2 Male germ cells regulation at the embryonic stage | 第14-15页 |
| 1.1.1.3 Balance between self-renewal and differentiation ofspermatogonial stem cells | 第15-21页 |
| 1.1.1.4 Meiosis associated gene expression in testis | 第21-25页 |
| 1.1.2 Other players during spermatogenesis | 第25-27页 |
| 1.1.2.1 Sertoli cell specification and differentiation | 第25-26页 |
| 1.1.2.2 Introduction of other testicular cells | 第26-27页 |
| 1.2 G4 structures and their related helicases | 第27-35页 |
| 1.2.1 G4 structures and their functions in vivo | 第27-33页 |
| 1.2.1.1 A brief introduction of G4 structures | 第27-29页 |
| 1.2.1.2 G4 structures in gene promoters and messenger RNAs | 第29-31页 |
| 1.2.1.3 Telomeres associated with G4 structures | 第31-32页 |
| 1.2.1.4 Other roles of G4 structures and their biologicalfunctions | 第32-33页 |
| 1.2.2 G4 helicases | 第33-35页 |
| 1.2.2.1 RHAU and its function | 第33-34页 |
| 1.2.2.2 Other helicases and their functions | 第34-35页 |
| Conclusions | 第35-36页 |
| References | 第36-54页 |
| Chapter Ⅱ: G-quadruplex DNA structure resolvase RHAU regulatesspermatogonia differentiation by facilitating c-kit transcription viatargeting G4 motifs at c-kit promoter | 第54-109页 |
| Introduction | 第55-58页 |
| Materials and Methods | 第58-68页 |
| Results | 第68-97页 |
| 2.1 G4 DNA structures exhibit a dynamic distribution at different stagesof spermatogenesis | 第68-69页 |
| 2.2 RHAU is expressed at multiple germ cell stages | 第69-72页 |
| 2.3 RHAU is essential for meiosis during spermatogenesis | 第72-75页 |
| 2.4 Meiosis is deficient in RHAU deleted mice | 第75-78页 |
| 2.5 Decreased differentiation of spermatogonia in RHAU deleted mice | 第78-81页 |
| 2.6 RHAU ablation in germ cells did not affect spermatogonia stem celldevelopment | 第81-85页 |
| 2.7 RHAU deletion reduced proliferation and enhanced apoptosis ofspermatogonia | 第85-89页 |
| 2.8 Preferential deregulation of genes carrying a G4 motif in theirpromoters in RHAU-ablated testes | 第89-92页 |
| 2.9 RHAU directly binds to G4 DNA motif at the promoter region of c-kit to enhance c-kit expression | 第92-97页 |
| Discussion | 第97-102页 |
| References | 第102-109页 |
| Acknowledgements | 第109-110页 |
| Original Publications | 第110-112页 |
