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Assessment of Genetic Diversity in Barley Germplasm and Sequencing of Ppd-H1Gene for Genetic Variation in Natural Populations of Barley from Middle East

ABSTRACT第5-7页
List of abbreviations第8-9页
List of tables and figures第9-14页
Chapter 1- Introduction第14-21页
    1.1. Morphology, taxonomy and origin of barley第14-15页
    1.2. Cultivation and Uses of the cultivated barley (Hordeum vulgare L.)第15-16页
        1.2.1. Cultivation of barley第15页
        1.2.2. Uses of barley第15-16页
    1.3. Domestication of barley第16-17页
    1.4. Importance of wild barley第17-18页
    1.5. Importance of landraces barley第18-21页
Chapter 2- Review of Literature第21-29页
    2.1. Genetic diversity based on molecular markers第21-23页
    2.2. Genetic diversity based on biochemical markers第23-25页
    2.3. Genetic diversity revealed by quantitative traits第25-27页
    2.4. Uses of ISJ primers第27-29页
Chapter 3- Genetic diversity and structure analysis of wild and landrace barleys inJordan revealed by ISJ markers第29-44页
    3.1. Introduction第29页
    3.2. Material and methods第29-34页
        3.2.1. Plant materials第29-32页
        3.2.2. DNA isolation第32-33页
        3.2.3. Polymerase chain reaction and gel electrophoresis第33页
        3.2.4. Data analysis第33-34页
    3.3. Results第34-41页
        3.3.1. Distribution of alleles and marker diversity第34-35页
        3.3.2. Genetic diversity and genetic differentiation within and among populations第35-36页
        3.3.3. Principle coordinates analysis (PCoA)第36-37页
        3.3.4. Genetic relationships第37-39页
        3.3.5. STRUCTURE analysis第39-41页
    3.4. Discussion第41-44页
        3.4.1. Genetic diversity第41-42页
        3.4.2. Genetic structure第42-44页
Chapter 4- Comparative assessment of seed storage protein diversity in Jordanianlandrace and wild barley第44-57页
    4.1. Introduction第44页
    4.2. Material and methods第44-46页
        4.2.1. Plant materials and protein extraction第44-45页
        4.2.2. Data analysis第45-46页
    4.3. Results第46-54页
        4.3.1. Extent and structure of hordein polymothism第46-47页
        4.3.2. Genetic similarity between the species and loci diversity第47-48页
        4.3.3. Genetic diversity and genetic differentiation within and among populations第48-50页
        4.3.4. Principle coordinates analysis(PCoA)and cluster analysis第50-52页
        4.3.5. STRUCTURE analysis第52-54页
    4.4. Discussion第54-57页
Chapter 5- Assessment of genetic diversity in barley germplasm based on quantitativetraits第57-69页
    5.1. Introduction第57页
    5.2. Material and method第57-59页
        5.2.1. Plant materials and agro-morphological characterization第57-58页
        5.2.2. Data analysis第58-59页
    5.3. Results第59-64页
        5.3.1. Quantitative trais diversity within and between species第59-60页
        5.3.2. Principle component analysis第60-62页
        5.3.3. Cluster analysis第62-63页
        5.3.4. Promising sources identified第63-64页
    5.4. Discussion第64-67页
    5.5. Quantitative traits and seed storage protein did not separate each population.Why?第67-69页
Chapter 6- Intra vs.interspecific crosses:A case study of genetic analysis for agronomictraits in barley第69-80页
    6.1. Introduction第69-70页
    6.2. Materials and methods第70-71页
        6.2.1. Plant materials and agronomic traits第70页
        6.2.2. Statistical analysis第70-71页
    6.3. Results第71-76页
        6.3.1. Mean squares and per se performance第71-73页
        6.3.2. Estimation of heterosis in F_1 hybrid populations第73页
        6.3.3. Heterobeltiosis estimates in intra and interspecific crosses第73-74页
        6.3.4. Estimation of heritability and genetic advance in F_1 hybrid populations第74-76页
        6.3.5. Inter-character correlations第76页
    6.4. Discussion第76-80页
        6.4.1. Per se performance第76页
        6.4.2. Genetic analysis of agronomic traits in inter and intraspecific crosses第76-78页
        6.4.3. Heterosis and genetic diversity第78-80页
Chapter 7- Effect of Ppd-H1 gene's SNP 48 markers on days to heading and associationdetected of SNPs with agro-morphological traits in barley natural populations fromMiddle East第80-90页
    7.1. Introduction第80-81页
    7.2. Materials and methods第81-83页
        7.2.1. Plant materials第81页
        7.2.2. DNA extraction第81页
        7.2.3. Primer,PCR and sequencing第81-82页
        7.2.4. Data analysis第82-83页
    7.3. Results第83-86页
        7.3.1. Variations in days to heading第83页
        7.3.2. Sequence results of SNP 48 marker第83-84页
        7.3.3. Identification of SNPs第84-86页
    7.4. Discussion第86-88页
    7.5. Conclusion第88-90页
Referenees第90-102页
Acknowledgment第102-104页
CURRICULUM VITAE第104-105页

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