| Table of Contents | 第1-7页 |
| DEDICATION | 第7-8页 |
| DECLARATION | 第8-9页 |
| Certification | 第9-10页 |
| Acknowledgement | 第10-13页 |
| Abstract English | 第13-16页 |
| Abstract Chinese | 第16-18页 |
| List of tables | 第18-19页 |
| List of figures | 第19-20页 |
| CHAPTER I: GENERAL INTRODUCTION AND OBJECTIVES OF STUDY | 第20-24页 |
| ·Introduction | 第20-22页 |
| ·Objcctives and Signineanee of Study | 第22-24页 |
| CHAPTER II: LITERATURE REVIEW | 第24-52页 |
| ·HBV Genotypes | 第24-36页 |
| ·HBX Gene Mutions and HCC | 第36-52页 |
| CHAPTER III: MATERIALS AND METHODS | 第52-66页 |
| ·Chemical Reagents | 第52页 |
| ·Analytical Methods | 第52-66页 |
| ·Extraction of Human Genomic DNA | 第52-55页 |
| ·Method A | 第52-54页 |
| ·Method B | 第54-55页 |
| ·Nested-PCR amplification of HBV genotypes | 第55-58页 |
| ·Nested-PCR Amplification of HBV pre-S gene | 第58-59页 |
| ·Nested-PCR Amplification of HBV X Gene | 第59-62页 |
| ·TALigation | 第62-63页 |
| ·Transformation of competent cells (JM109/DH5a) | 第63-64页 |
| ·Colony selection | 第64页 |
| ·Identification of cloned gene (target DNA) | 第64-66页 |
| CHAPTER IV: RESULTS AND ANALYSIS | 第66-78页 |
| PART I: 4.1 Characterization and distribution of HBV genotypes | 第66-70页 |
| ·Distribution of HBV genotypes | 第66-67页 |
| ·Association of liver diseases and prevalence of HBV genotypes | 第67-70页 |
| PART II: 4.2 Characterization of HBV X gene mutation in subjects with HCC | 第70-78页 |
| ·Negative nested-PCR signal from serum samples from subjects with HCC | 第70-71页 |
| ·HBV X gene amplification from human genomic DNA, cloning and sequencing | 第71-78页 |
| CHAPTER V: DISCUSSION | 第78-89页 |
| PART I: 5.1 Characterization and distribution of HBV genotypes | 第78-84页 |
| PART II: 5.2 Characterization of HBV X gene mutation in subjects with HCC | 第84-89页 |
| CHAPTER VI: CONCLUSIONS AND SUGGESTIONS | 第89-95页 |
| PART I: 6.1 Characterization and distribution of HBV genotypes | 第89-92页 |
| PART II: 6.2 Characterization of HBV X gene mutations in subjects with HCC | 第92-95页 |
| CHAPTER VII: REFERENCES | 第95-106页 |
| APPENDIX 1: COMMON ABBREVIATION USED IN BIOCHEMISTRY, MOLECULAR BIOLOGY, VIROLOGY, IMMUNOLOGY,MEDICINE | 第106-109页 |
| APPENDIX 2: EDUCATIONAL BACKGROUND | 第109-111页 |
| REVIEW | 第111-131页 |
