ABSTRACT | 第8-9页 |
摘要 | 第10-12页 |
CHAPTER ONE: INTRODUCTION AND LITERATURE REVIEW | 第12-26页 |
1 Plant hormones classification | 第14-24页 |
1.1 Natural hormones | 第14-16页 |
1.2 Artificial hormones | 第16-17页 |
1.3 The molecular mechanisms governing plant hormone actions | 第17页 |
1.4 Hormone action in growth and organ development | 第17-19页 |
1.5 The molecular basis of hormone biosynthesis, metabolism, and its regulation | 第19页 |
1.6 Hormone perception and signal transduction | 第19页 |
1.7 Signal cross-talks in hormone actions | 第19-20页 |
1.8 Hormonal regulation of plant responses to biotic and abiotic stresses | 第20-21页 |
1.9 Hormonal interactions in the regulation of plant development | 第21页 |
1.10 Detection of hormones in plants tissues | 第21-24页 |
2 Objectives of this study | 第24页 |
2.1 General objectives | 第24页 |
2.2 Specific objectives | 第24页 |
3 Problem statement | 第24-25页 |
4 Hypotheses | 第25-26页 |
CHAPTER TWO: THE EFFECTS OF PGRs ON THE GROWTH OF THREERADISH CULTIVARS WITH DIFFERENT TAPROOT SHAPE AND COLOR | 第26-46页 |
1 Introduction | 第26-28页 |
2 Materials and methods | 第28-31页 |
2.1 Plant materials | 第28-29页 |
2.2 Measurements: (Root measurement) | 第29-30页 |
2.3 Shoot measurement | 第30页 |
2.4 Chlorophyll measurement | 第30-31页 |
2.5 Data analysis | 第31页 |
3 Results | 第31-42页 |
3.1 Morphological comparisons between radish cultivars | 第33-35页 |
3.2 Effect of applications 6-benzylaminopurine (6-BA) and melatonin (MT) onradish | 第35-41页 |
3.3 Measurement of chlorophyll | 第41-42页 |
4 Discussion | 第42-44页 |
5 Conclusion | 第44-46页 |
CHAPTER THREE: EFFECTS OF GA_3 AND PACLOBUTRAZOL (PBZ)APPLICATION ON RADISH (Raphanus sativus L.) TAPROOT FORMATION | 第46-70页 |
1 Introduction | 第46-48页 |
2 Materials and methods | 第48-52页 |
2.1 Plant material | 第48-49页 |
2.2 Growth and photosynthesis net rate determination | 第49-50页 |
2.3 Paraffin sections and microtome histology | 第50页 |
2.4 Preparation and extraction of endogenous hormones | 第50-51页 |
2.5 Mass spectrometry analysis of plant hormones | 第51页 |
2.6 Total RNA extraction and cDNA synthesis | 第51页 |
2.7 Gene expression analysis by quantitative real-time PCR | 第51-52页 |
2.8 Statistical analysis | 第52页 |
3 Results | 第52-62页 |
3.1 Plant features and growth parameters | 第52-55页 |
3.2 Photosynthesis activity | 第55-56页 |
3.3 Histological analysis | 第56-59页 |
3.4 Analysis of hormones in root and leaves by LC-MS | 第59-61页 |
3.5 Expression analysis of gibberellin biosynthesis and metabolism genes in radishgenotypes | 第61-62页 |
4 Discussion | 第62-69页 |
4.1 DELLA proteins repressors of GA signaling | 第67-68页 |
4.2 The interplay between GA and auxin signaling systems | 第68页 |
4.3 Gibberellin signal receptors | 第68-69页 |
5 Conclusions | 第69-70页 |
GENERAL CONCLUSION | 第70-72页 |
REFERENCES | 第72-82页 |
PUBLICATIONS | 第82-83页 |
ACKNOWLEDGEMENTS | 第83页 |