| Abstract in English | 第6-7页 |
| Abstract in Chinese | 第8-10页 |
| List of Abbreviations | 第10-11页 |
| 1 Introduction | 第11-31页 |
| 1.1 The origin of the research issue | 第11-13页 |
| 1.2 Literature review | 第13-30页 |
| 1.2.1 Responses of plant under abiotic stresses | 第13-17页 |
| 1.2.1.1 Responses and signal transduction of plants under abiotic stresses | 第13-15页 |
| 1.2.1.2 Regulatory network of gene expression under abiotic stresses | 第15-17页 |
| 1.2.2 The role of ABA on abiotic stress response in plant | 第17-20页 |
| 1.2.3 Genetic transformation in plants | 第20-21页 |
| 1.2.4 Progress of technology study for the introduction of multiple genes into transgenic plants | 第21-24页 |
| 1.2.5 Candidate genes for genetic transformation | 第24-30页 |
| 1.2.5.1 bZIP transcription factor-encoding genes | 第25-28页 |
| 1.2.5.2 SAPK protein kinase-encoding genes | 第28-30页 |
| 1.3 Research purpose and significance | 第30-31页 |
| 2 Materials and methods | 第31-40页 |
| 2.1 Experimental materials | 第31-32页 |
| 2.2 Agrobacterium-mediated transformation | 第32页 |
| 2.3 DNA extraction and PCR positive test | 第32-33页 |
| 2.4 RNA extraction, reverse transcription and Real-time PCR | 第33-34页 |
| 2.5 Southern blot hybridization | 第34-35页 |
| 2.6 ABA sensitivity test of the transgenic plants | 第35-36页 |
| 2.7 Stress tolerance experiments of transgenic rice at the seedling stage | 第36页 |
| 2.7.1 Drought resistance experiment of transgenic rice at the seedling stage | 第36页 |
| 2.7.2 Temperature stress resistance experiments of transgenic rice at the seedling stage | 第36页 |
| 2.8 Drought resistance experiment of transgenic rice at the reproductive stage | 第36-37页 |
| 2.9 Determination of stress-related physiological indicators in rice | 第37-38页 |
| 2.9.1 Determination of water loss rate in rice leaves | 第37页 |
| 2.9.2 Determination of cell membrane permeability | 第37-38页 |
| 2.9.3 Measure content of malondialdehyde (MDA) in rice leaves | 第38页 |
| 2.10 RNA-Seq and analysis | 第38-40页 |
| 3 Results | 第40-60页 |
| 3.1 Screening of candidate genes and stress resistance in the transgenic plants | 第40-44页 |
| 3.1.1 Identification the presence of candidate genes in the transgenic plants | 第40页 |
| 3.1.2 Expression level analysis of candidate genes in the transgenic plants | 第40-43页 |
| 3.1.3 Copy number analysis of the partial T1 transgenic plants | 第43-44页 |
| 3.1.4 Phenotypic pre-evaluation of transgenic materials in the field | 第44页 |
| 3.2 Increased ABA sensitivity of XL22 co-overexpression seedlings | 第44-47页 |
| 3.3 Enhanced drought resistance of the XL22 transgenic seedlings | 第47-49页 |
| 3.4 Enhanced drought resistance of the XL22 transgenic plants at the reproductive stage | 第49-52页 |
| 3.5 The XL22 transgenic lines improved heat and cold tolerance | 第52-55页 |
| 3.6 Enhanced oxidation resistance of XL22 plants under heat stress | 第55-56页 |
| 3.7 Transcriptome profiling of the XL22 transgenic plants | 第56-60页 |
| 4 Discussion | 第60-69页 |
| 4.1 Co-overexpression of OsbZIP46CA1 and SAPK6 enhances drought tolerance | 第60-63页 |
| 4.2 Rice drought resistance evaluation indicators | 第63-64页 |
| 4.3 Co-overexpression of OsbZIP46CA1 and SAPK6 improves tolerance to temperature stress | 第64-66页 |
| 4.4 Application of multi-gene assembly strategy in improving stress resistance of rice | 第66-69页 |
| References | 第69-86页 |
| Appendices | 第86-106页 |
| Appendix 1. Supplemental figures | 第86-88页 |
| Appendix 2. Supplemental datas | 第88-99页 |
| Appendix 3. Protocols | 第99-106页 |
| Protocol 1. Plasmid DNA extraction | 第99页 |
| Protocol 2. Extraction of total DNA using Plant DNAzol reagent | 第99-101页 |
| Protocol 3. Southern blot hybridization | 第101-106页 |
| Resume | 第106-109页 |
| Acknowledgement | 第109-111页 |
