| 摘要 | 第1-8页 |
| Abstract | 第8-16页 |
| CHAPTER Ⅰ Introduction | 第16-25页 |
| ·NAC family | 第16-18页 |
| ·General information of NAC family | 第16-17页 |
| ·Role of NAC family genes in plant | 第17-18页 |
| ·Leaf senescence | 第18-19页 |
| ·Leaf senescence as programmed cell death | 第19-20页 |
| ·Analysis and regulation of leaf senescence | 第20-22页 |
| ·Genetic analysis of leaf senescence | 第20页 |
| ·Molecular approaches for leaf senescence | 第20-21页 |
| ·Molecular genetic regulation of leaf senescence | 第21-22页 |
| ·Onset of leaf senescence | 第22页 |
| ·Environmental factors and hormones regulating leaf senescence | 第22-24页 |
| ·Environmental factors regulating leaf senescence | 第22-23页 |
| ·Phytohormones pathways in leaf senescence | 第23-24页 |
| ·NAC family and leaf senescence | 第24页 |
| ·Research objectives | 第24-25页 |
| CHAPTER Ⅱ Characterization of GhNAC8-Gh2NAC17 genes for leaf senescence andresponses to stresses | 第25-46页 |
| ·Materials and Methods | 第25-28页 |
| ·Plant material,growth conditions,and stress assays | 第25页 |
| ·RNA extraction,cDNA synthesis and DNA preparation | 第25-26页 |
| ·Amplification of internal conserved sequences of GhNACs | 第26页 |
| ·Sequence analysis | 第26页 |
| ·Isolation of GhNAC promoters by genome walking method | 第26页 |
| ·Quantitative RT-PCR assays | 第26-27页 |
| ·Chlorophyll measurement | 第27页 |
| ·Subcellular localization | 第27-28页 |
| ·GhNAC8 and GhNAC9 plasmid construction and transgenic lines | 第28页 |
| ·Statistical analysis | 第28页 |
| ·Results | 第28-41页 |
| ·Cloning of GhNAC transcription factor genes from upland cotton | 第28-29页 |
| ·Sequence analysis of the deduced GhNAC proteins | 第29-30页 |
| ·Phylogenic analysis of GhNACs at the protein level | 第30-32页 |
| ·Expression analysis of GhNACs in different organs | 第32-33页 |
| ·Morphological and chlorophyll response during natural and induced leaf senescence | 第33-34页 |
| ·Expression of GhNACs genes during induced leaf senescence in cotton | 第34-35页 |
| ·Expression of GhNACs genes during natural leaf senescence in cotton | 第35页 |
| ·Expression of GhNACs genes under abiotic stresses | 第35-37页 |
| ·Hormones treatments on the expression of GhNACs genes | 第37-38页 |
| ·Study of partial putative cis-acting elements of GhNACs gene | 第38页 |
| ·Nuclear localization of GhNAC9 and GhNAC10 proteins | 第38-39页 |
| ·Ectopic expression of GhNAC8 and GhNAC9 in Arabidopsis | 第39-41页 |
| ·Summary of GhNAC18-GhNAC17 genes results | 第41页 |
| ·Discussion | 第41-46页 |
| CHAPTER Ⅲ Expression analysis of NAC family gene(GhNAC18-77)for multipleabiotic stresses in cotton | 第46-67页 |
| ·Materials and Methods | 第46-47页 |
| ·Database search and sequence retrieval | 第46页 |
| ·Phylogenetic analysis | 第46页 |
| ·Chromosome location | 第46页 |
| ·Plant growth and stress assays | 第46-47页 |
| ·RNA extraction and cDNA synthesis | 第47页 |
| ·Amplification of internal conserved sequences of GhNACs | 第47页 |
| ·Quantitative RT-PCR assays | 第47页 |
| ·Results | 第47-63页 |
| ·Identification of NAC gene family in Gossypium hirsutum L | 第47-48页 |
| ·Phylogenetic analysis of GhNAC genes | 第48-51页 |
| ·Chromosome location of GhNAC genes | 第51-52页 |
| ·Motifs and phylogenetic tree of GhNAC genes | 第52-54页 |
| ·Expression profile of GhNACs in different organs | 第54-55页 |
| ·Morphological and chlorophyll response during natural leaf senescence and GhNAC genes expression | 第55-57页 |
| ·GhNAC genes expression by exogenous application of phytohormones | 第57-60页 |
| ·GhNAC genes expression under drought and salt stresses | 第60-63页 |
| ·Expression profile of GhNAC in developing fibre | 第63页 |
| ·Discussion | 第63-67页 |
| CHAPTER Ⅳ GhNAC7,a novel cotton NAC family transcription factor gene has animportant role in leaf senescence | 第67-81页 |
| ·Materials and Methods | 第67-70页 |
| ·Plant material,growth conditions,and stress assays | 第67页 |
| ·RNA extraction,cDNA synthesis and DNA preparation | 第67页 |
| ·GhNAC7 gene amplification and sequence analysis | 第67页 |
| ·Isolation of GhNAC7 promoter | 第67页 |
| ·Quantitative RT-PCR assays | 第67页 |
| ·Chlorophyll measurement | 第67页 |
| ·Subcellular localization of GhNAC7 | 第67-68页 |
| ·Constructs of plasmid and transformation of Arabidopsis | 第68页 |
| ·Transformation of Arabidopsis | 第68-69页 |
| ·Histochemical GUS staining | 第69页 |
| ·Transformation of tobacco | 第69页 |
| ·Transformation of cotton(Gossypium hirsutum L.) | 第69-70页 |
| ·In-vitro binding protein assay and SDS-PAGE gel electrophoresis | 第70页 |
| ·Chromatin immunoprecipitation(ChIP)assay | 第70页 |
| ·Statistical analysis | 第70页 |
| ·Results | 第70-79页 |
| ·GhNAC7 gene in Gossypium hirsutum L | 第70-71页 |
| ·Nuclear localization of GFP-GhNAC7 protein | 第71页 |
| ·Expression pattern of GhNAC7 in upland cotton | 第71-72页 |
| ·Expression of GhNAC7 during abiotic stresses | 第72-73页 |
| ·Phenotypes of GhNAC7overexpression in Arabidopsis | 第73-74页 |
| ·Expression pattern of GhNAC7 conferred by their promoter | 第74-75页 |
| ·GhNAC7overexpression in tobacco | 第75-76页 |
| ·Overexpression transgenic lines of GhNAC7 in Gossypium hirsutum L | 第76-77页 |
| ·The pGhNAC7 has positive interaction with GhNAC7 protein | 第77-78页 |
| ·In vitro DNA/protein-binding assay and immunoblot analysis | 第78-79页 |
| ·Discussion | 第79-81页 |
| CONCLUSION | 第81-82页 |
| REFERENCES | 第82-91页 |
| SUPPLEMENTARY DATA | 第91-114页 |
| ACKNOWLEDGEMENTS | 第114-115页 |
| CURRICULUM VITAE | 第115页 |
