| Abstract | 第1-7页 |
| 摘要 | 第7-11页 |
| CHAPTER 1: Review on nucleosides of Cordyceps and analytical methods | 第11-25页 |
| Section 1: Review of nucleosides study in Cordyceps | 第11-13页 |
| ·Introduction | 第11页 |
| ·Natural Cordyceps. Sinensis, cultured Cordyceps. Sinensis and cultured Cordyceps. Sinensis | 第11-12页 |
| ·Nucleoside-one of the major components in Cordyceps | 第12页 |
| ·Nucleosides - authentication of Cordyceps | 第12-13页 |
| Section 2: Review on analytical methods for nucleosides and analogous | 第13-25页 |
| ·Chromatographic analysis of nucleosides | 第14-23页 |
| ·Electrochemistry analytical methods in nucleosides | 第23页 |
| ·Biological analytical methods in nucleosides | 第23-24页 |
| ·The perspective of the new analytical methods of nucleosides in Cordyceps | 第24-25页 |
| CHAPTER 2: Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressu rized liquid extraction and high performance liquid chromatography-el ectrospray ionization tandem mass spectrometr | 第25-47页 |
| Section 1: Materials and instruments | 第25-29页 |
| ·Materials | 第25-26页 |
| ·Instruments | 第26-29页 |
| Section 2: Methods and sample preparation | 第29-30页 |
| ·Sample preparation | 第29页 |
| ·HPLC-UV-MS analysis | 第29-30页 |
| Section 3: Results and discussion | 第30-47页 |
| ·Qualitative analysis on nucleotides in Cordyceps by HPLC-DAD-ESI-MS | 第30-39页 |
| ·Qualitative analysis of components in Cordyceps by HPLC-DAD-ESI-MS | 第39-47页 |
| CHAPTER 3: Comparison of bio-available nucleosides compounds in natural and cultured Cordyceps using HPLC-DAD | 第47-79页 |
| Section 1: Materials and methods | 第50-67页 |
| ·Materials | 第50页 |
| ·Instruments | 第50-51页 |
| ·Optimization of chromatographic condition | 第51-56页 |
| ·Optimization of acid hydrolytic condition | 第56-62页 |
| ·Optimization of sample preparation condition | 第62-67页 |
| Section 2: Results and discussion | 第67-77页 |
| ·Qualitative analysis using LC-MS | 第68-73页 |
| ·Validation of the method | 第73-75页 |
| ·Quantitation of bio-available nucleosides in Cordyceps | 第75-77页 |
| Section 3: Discussion | 第77-79页 |
| CHAPTER 4: Evaluation of nucleosides on quality control of Cordyceps | 第79-82页 |
| Section 1: Comparison of the amounts of nucleosides using LC-MS method and bio-available nucleosides using HPLC method | 第79-80页 |
| Section 2: The analysis of nucleosides on quality control of Cordyceps | 第80-82页 |
| Reference | 第82-91页 |
| Appendix I:UV,TIC,EIC,MRM | 第91-116页 |
| Appendix II:UV chromtaograms of hydrolytic Cordyceps using HPLC-DAD | 第116-132页 |
| LIST OF ABBREVIATIONS | 第132-133页 |
| LIST OF FIGURES | 第133-135页 |
| LIST OF TABLES | 第135-136页 |
| 在学期间完成论文及参加学术会议 | 第136-137页 |
| 致谢 | 第137页 |
