| 摘要 | 第5-7页 |
| ABSTRACT | 第7-8页 |
| TABLE OF CONTENTS | 第9-12页 |
| LIST OF TABLES | 第12-13页 |
| LIST OF FIGURES | 第13-16页 |
| ABREVIATIONS | 第16-17页 |
| CHAPTER Ⅰ: LITERATURE REVIEW | 第17-34页 |
| 1.1 Cotton Verticilium Wilt Disease | 第17-20页 |
| 1.1.1 Cotton Crop (Gossypium spp) | 第17-18页 |
| 1.1.2 Verticillium dahliae Kleb | 第18-20页 |
| 1.2 Secreted Virulence Factors in Verticilium dahliae Kleb | 第20-31页 |
| 1.2.1 Verticilium dahliae Genome | 第20-21页 |
| 1.2.2 Necrosis and Ethylene-inducing Proteins (VdNEP) Description | 第21-26页 |
| 1.2.3 Carbohydrate Active Enzymes (CAZY) Family | 第26-31页 |
| 1.3 Vascular wilt Diseases in Cotton | 第31-33页 |
| 1.4 Research Objectives | 第33-34页 |
| 1.4.1 Screening and identification of potential virulence genes in Verticilium dahliae cottonisolate | 第33页 |
| 1.4.2 Functional and Molecular characterization of some identified virulence genes in cotton V. dahliae | 第33-34页 |
| CHAPTER Ⅱ: FUNCTIONAL ANALYSIS OF NECROSIS AND ETHYLENE-INDUCING PROTEIN (VDNEP6) IN VERTICILLIUM DAHLIAE KLEB, STRAINS | 第34-51页 |
| 2.1 Summary | 第34页 |
| 2.2 Introduction | 第34-35页 |
| 2.3 Materials and Methods | 第35-38页 |
| 2.3.1 Fungal strains and growth conditions | 第35-36页 |
| 2.3.2 Nucleic Acid Manipulation | 第36页 |
| 2.3.3 Isolation of VdNEP6 DNA fragment from V. dahliae | 第36页 |
| 2.3.4 Functional Validation of VdNEP6 Signal peptide | 第36-37页 |
| 2.3.5 VdNEP6 Transient expression | 第37-38页 |
| 2.3.6 Transformation-mediated mutagenesis of VdNEP6 gene | 第38页 |
| 2.3.7 Growth activity and pathogenicity assays | 第38页 |
| 2.4 Results | 第38-49页 |
| 2.4.1 Gene structure and functional domain analysis | 第38-39页 |
| 2.4.2 Detection of Single Nucleotide Polymorphism (SNPs) among V. dahliae Isolates | 第39-41页 |
| 2.4.3 Functional Validation of VdNEP6 Signal Peptide | 第41页 |
| 2.4.4 Transient Expression of VdNEP6 in Tobacco leaves | 第41-43页 |
| 2.4.5 Vegetative growth on different carbon sources media | 第43-45页 |
| 2.4.6 Transformation-mediated disruption of the VdNEP6 gene | 第45-48页 |
| 2.4.7 Disease assay of the VdNEP6 mutants on cotton | 第48-49页 |
| 2.5 Discussion | 第49-51页 |
| CHAPTER Ⅲ: FUNCTIONAL CHARACTERIZATION OF CUTINASE ANDCARBOXYPEPTIDASE Y CANDIDATE GENES IN COTTON VERTICILLIUMDAHLIAE STRAIN | 第51-64页 |
| 3.1 Summary | 第51页 |
| 3.2 Introduction | 第51-53页 |
| 3.3 Materials and Methods | 第53-55页 |
| 3.3.1 Strains and Growth Conditions | 第53页 |
| 3.3.2 Nucleic Acid Manipulation | 第53页 |
| 3.3.3 CAZy's Targeted candidates Genes DNA fragment Isolation | 第53-54页 |
| 3.3.4 CAZy's Candidate genes Transcripts Expression | 第54页 |
| 3.3.5 CAZy's Candidate genes Transient expression | 第54-55页 |
| 3.3.6 V. dahliae Transformation and candidates genes Disruption | 第55页 |
| 3.4 Results | 第55-62页 |
| 3.4.1 Candidate gene selection and cloning | 第55-56页 |
| 3.4.2 CAZy candidate Genes | 第56-58页 |
| 3.4.3 Transient Expression and Suppression activity of CAZy's candidates genes | 第58-60页 |
| 3.4.4 Transcript Expression | 第60-61页 |
| 3.4.5 Fungal Transformation and Cutinase and Carboxypeptidase Y disruption | 第61-62页 |
| 3.5 Discussion | 第62-64页 |
| CHAPTER Ⅳ: EDENTIFICATION AND FUNCTIONAL CHARACTERIZATION OFVASCULAR RELATED GENES IN COTTON VERTICILIUM DAHLIAE, KLEB | 第64-83页 |
| 4.1 Summary | 第64页 |
| 4.2 Introduction | 第64-66页 |
| 4.3 Materials and Methods | 第66-70页 |
| 4.3.1 Identification of Vascular Specific Genes | 第66-67页 |
| 4.3.2 Fungal strains and growth conditions | 第67-68页 |
| 4.3.3 Nucleic Acid Manipulation | 第68页 |
| 4.3.4 Isolation of Vascular related genes DNA fragments from V. dahliae | 第68页 |
| 4.3.5 Vascular related genes disruption using ATMT technique | 第68-69页 |
| 4.3.6 Growth and Conidiation Activity | 第69页 |
| 4.3.7 Pathogenicity Assessment of Vascular Related Genes in Cotton | 第69-70页 |
| 4.4 Results | 第70-81页 |
| 4.4.1 Identification of vascular related genes | 第70-72页 |
| 4.4.2 Genes expression analysis | 第72-73页 |
| 4.4.3 Vascular related genes disruption using ATMT technique | 第73-76页 |
| 4.4.4 Growth and Conidiation Activity | 第76-78页 |
| 4.4.5 Pathogenicity Assessment of Vascular Related Genes in Cotton | 第78-81页 |
| 4.5 Discussion | 第81-83页 |
| GENERAL CONCLUSIONS | 第83-85页 |
| REFERENCES | 第85-102页 |
| ACKNOWLEDGEMENTS | 第102-103页 |
| RESUME | 第103-107页 |
