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霜霉病菌处理后不结球白菜相关抗性基因表达分析

ABSTRACT第6-7页
摘要第8-10页
ABBREVIATION第10-11页
Chapter Ⅰ literature Review第11-25页
    1. Plants immune resistance against downy mildew第11-13页
    2. PEN2 is important primary immune response to Oomycetes第13-14页
    3. U-box proteins as a new defense family第14-19页
    4. SGT1 is required for R genes signaling in plants第19-21页
    5. Enhanced Disease Susceptibility 1 (EDS1) controls defense activation and programmed cell death to fungus第21-22页
    6. Research Objectives第22-23页
    References第23-25页
Chapter Ⅱ PEN2, EDS1,AND SGT1b gene expression analysis in non-heading Chinese cabbage plants treated with Hyaloperonospora parasitica第25-35页
    1. Materials and Methods第26-29页
        1.1. Plant Materials第26页
        1.2. Isolation of total RNA第26-27页
        1.3. Cloning of full-length cDNA of PEN2, SGT1b and EDS1第27页
        1.4. Quantitative real-time PCR第27-28页
        1.5. Statistical analysis第28-29页
    2. Results第29-32页
        2.1. Alignment第29-30页
        2.2. Phylogenetic tree第30-31页
        2.3. RT-PCR第31-32页
    3. Discussion第32-33页
    REFERENCES第33-35页
Chapter Ⅲ PEN2 may have different expression in non-heading Chinese cabbage in response to downy mildew第35-47页
    1. Materials and Methods第36-39页
        1.1. Plant Materials第36页
        1.2. Isolation of total RNA第36-37页
        1.3. cDNA synthesis第37页
        1.4. Cloning of full-length cDNA of PEN2第37-38页
        1.5. Quantitative real-time PCR第38页
        1.6. Statistical analysis第38-39页
    2. Results第39-45页
        2.1. In siliques analysis第39-40页
        2.2. Phylogenetic tree第40-41页
        2.3. Leaves treatment with downy mildew第41-42页
        2.4. RT-PCR第42-44页
        2.5 Promoter comparison第44-45页
    3. Discussion第45-46页
    REFERENCES第46-47页
Chapter Ⅳ expression analysis of PUB genes in Non-heading Chinese cabbage resistance-related to downy mildew第47-57页
    1. Materials and Methods第49-50页
        1.1 RNA-seqtranscriptome data第49-50页
        1.2 Data Analysis第50页
        1.3 Cloning and identification of the BcPUBs in non-heading Chinese cabbage第50页
    2. Results第50-53页
    3. Discussion第53-55页
    REFERENCES第55-57页
MAIN CONCLUSIONS第57-59页
APPENDIX第59-65页
PUBLICATIONS第65-67页
ACKNOWLEGEMENTS第67页

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