| Abstract | 第1-7页 |
| Abbreviations | 第7-12页 |
| Chapter 1 Literature review | 第12-44页 |
| ·Concept of stem cell | 第12-13页 |
| ·Classification and sources of stem cells | 第13页 |
| ·Skin histology | 第13-18页 |
| ·Basic structure of the skin | 第15-18页 |
| ·Skin stem cell | 第18-21页 |
| ·Stem cell in the epidermis | 第18-19页 |
| ·The basal layer contains both stem cells and transit amplifying cells | 第19-21页 |
| ·In vitro propagation of Epidermal keratinocytes | 第21-23页 |
| ·Keratinocyte culture systems in vitro | 第22-23页 |
| ·Stem cell selection | 第23页 |
| ·Epidermal differentiation | 第23-26页 |
| ·Epidermal differentiation in vitro | 第24-26页 |
| ·Hair follicle stem cells | 第26-30页 |
| ·Multipotent stem cell niche in hair follicle | 第28-29页 |
| ·Relationship between bulge and interfollicular stem cells | 第29-30页 |
| ·Stem cell cycle | 第30-31页 |
| ·Functional Characteristics of Keratinocyte stem cells | 第31-34页 |
| ·Factors regulating the skin stem cell | 第34-39页 |
| ·Wnt singnaling | 第35-36页 |
| ·Notch Signaling | 第36-37页 |
| ·Hedgehog Signaling | 第37-38页 |
| ·BMP Signaling | 第38-39页 |
| ·Clinical application of keratinocyte stem cell | 第39-41页 |
| ·Conclusion | 第41-44页 |
| Chapter 2 Keratinocytes isolation, culture and propagation | 第44-71页 |
| ·Introduction | 第44-45页 |
| ·Materials and Methods | 第45-53页 |
| ·Fibroblasts culture as feeder cell | 第45-49页 |
| ·Keratinocytes isolation and culture | 第49-52页 |
| ·Statistical analysis | 第52-53页 |
| ·Results | 第53-65页 |
| ·Cultivation of fibroblasts | 第53-55页 |
| ·Morphology of fibroblasts | 第55页 |
| ·Growth curve | 第55-57页 |
| ·Chromosome analysis | 第57页 |
| ·Cultivation of epidermal keratinocytes on feeder layer | 第57-58页 |
| ·Effect of plating time and density of feeder cells on keratinocyte growth | 第58-59页 |
| ·Proliferative potential of basal epidermal keratinocytes | 第59-60页 |
| ·Keratinocyte culture without feeder layer | 第60-61页 |
| ·Keratinocytes growth assay | 第61-62页 |
| ·Morphology of keratinocytes | 第62-63页 |
| ·Keratinocytes isolation from ORS of plucked hair follicle | 第63-65页 |
| ·Discussion | 第65-71页 |
| ·Fibroblasts culture | 第65-66页 |
| ·Keratinocyte culture | 第66-71页 |
| Chapter 3 Putative epidermal stem cell selection, propagation and characterization | 第71-87页 |
| ·Introduction | 第71页 |
| ·Materials and Methods | 第71-75页 |
| ·Stem cell selection in keratinocyte culture by clonal analysis | 第71-72页 |
| ·Stem cell isolation by rapid adherence of keratinocytes on collagen IV coated plate | 第72-74页 |
| ·Statistical analysis | 第74-75页 |
| ·Results | 第75-83页 |
| ·Clonogenic ability of keratinocytes and selection of holoclones as stem cells | 第75-76页 |
| ·Putative epidermal stem cell selection by the adherence on collagen IV substrate | 第76-78页 |
| ·Effects of ionic calcium concentration on growth | 第78-79页 |
| ·Colony formation efficiency of selected population | 第79-81页 |
| ·Karyotype study | 第81页 |
| ·Morphology of collagen enriched cells | 第81-83页 |
| ·Discussion | 第83-87页 |
| Chapter 4 Identification of epidermal stem cells by candidate molecular marker expressi | 第87-96页 |
| ·Introduction | 第87页 |
| ·Materials and Methods | 第87-89页 |
| ·Immunofluorescence staining | 第87-89页 |
| ·Statistical analysis | 第89页 |
| ·Result | 第89-93页 |
| ·Molecular marker expression in stem cells | 第89页 |
| ·Markers expression in spontaneous differentiated cells | 第89-93页 |
| ·Discussion | 第93-96页 |
| Chapter 5 Directional differentiation of skin stem cell | 第96-115页 |
| ·Introduction | 第96页 |
| ·Materials and Methods | 第96-105页 |
| ·Epidermis-derived stem cell induction for reconstruction of skin in organotypic culture | 第96-103页 |
| ·Dermis-derived stem cell induction | 第103-105页 |
| ·Results | 第105-111页 |
| ·Reconstructed epidermis by epidermal stem cells | 第105-106页 |
| ·Dermis-derived stem cells differentiated to insulin producing pancreatic ce | 第106页 |
| ·Skin derved precursor cells (SKPs) differentiated to neurons | 第106-111页 |
| ·Discussion | 第111-115页 |
| Chapter 6 Summary, Conclusion & Innovation | 第115-119页 |
| References | 第119-139页 |
| Appendix: General materials for experiment | 第139-143页 |
| ACKNOWLEDGEMENTS | 第143-146页 |
| VITA | 第146页 |
