| ABSTRACT | 第3-6页 |
| DECLARATION | 第7-8页 |
| DEDICATIONS | 第8-9页 |
| ACKNOWLEDGEMENTS | 第9-12页 |
| CONTENTS | 第12-15页 |
| ABBREVIATIONS | 第15-19页 |
| Chapter.1 INTRODUCTION | 第19-22页 |
| Chapter.2 REVIEW OF THE LITERATURE | 第22-67页 |
| 2.1.Plant Classification Systems and Plant Taxonomy | 第22-23页 |
| 2.1.1.History of Plant Taxonomy | 第22-23页 |
| 2.2.Morphological Classification System(MCS) | 第23-25页 |
| 2.3.Chemical Classification System(CCS) | 第25-27页 |
| 2.4.Molecular Systematic Approaches(MSA) | 第27-30页 |
| 2.5.Ethnobotanical Study and Drug Discovery | 第30-33页 |
| 2.5.1.Ethnobotany in China | 第31-32页 |
| 2.5.2.Significance of Ethnobotany | 第32-33页 |
| 2.6.Botanical Drugs/TCMs and Quality Control(QC) | 第33-37页 |
| 2.7.Botanical Informations of Medicinal Plants Studied | 第37-67页 |
| 2.7.1.Genus Houttuynia | 第37-40页 |
| 2.7.2.Genus Clematis | 第40-61页 |
| 2.7.3.Genus Ranunculus | 第61-64页 |
| 2.7.4.Genus Paeonia | 第64-67页 |
| Chapter 3.AIMS OF STUDY | 第67-69页 |
| Chapter 4.EXPERIMENTAL | 第69-107页 |
| 4.1 Materials and Equipments | 第69-76页 |
| 4.1.1.Morphological Approach | 第69-71页 |
| 4.1.2.Chemical Approach | 第71-74页 |
| 4.1.3.Proteomic Approach | 第74-76页 |
| 4.2.METHODS | 第76-107页 |
| 4.2.1.Phylogenetic Analysis of Genus Clematis Taxa Based on Morphological Data(Ⅲ) | 第76-80页 |
| 4.2.2.Chemical Analysis of C.huchouensis Tamura by HPLC-UV(Ⅴ) | 第80-82页 |
| 4.2.3.Chemical Analysis of Genus Clematis Taxa by LC-UV-MS(Ⅳ) | 第82-85页 |
| 4.2.4.Ethnobotanical Survey of TMSBR and Its Significance(Ⅱ) | 第85-88页 |
| 4.2.5.Chemical Analysis of Houttuynia cordata by GC/MS(Ⅰ) | 第88-91页 |
| 4.2.6.Protocol Optimization and Evaluation for Proteome Analysis of Clematis taxa(Ⅵ) | 第91-95页 |
| 4.2.7.Proteome Analysis by Two-dimensional Electrophoresis Technique and Intra-genus(Clematis)Classification(Ⅵ) | 第95-104页 |
| 4.2.8.Proteome Analysis by 2-DE Technique to Differentiate TCM Herb(C.chinensis)from Allies by Identifying Bio-markers(Ⅶ) | 第104-105页 |
| 4.2.9.Proteome Analysis by 2-DE Technique and Inter-genera Classification(Ⅷ) | 第105-107页 |
| Chapter 5.RESULTS | 第107-174页 |
| 5.1.Phylogenetic Analysis Based on Morphological Data | 第107-108页 |
| 5.2.Chemical Analysis of C.huchouensis by HPLC-UV and Its QC | 第108-113页 |
| 5.2.1.UV Spectra and Chromatographic Separation | 第109页 |
| 5.2.2.Selection of Reference Peak and Common Peak Fractions | 第109-111页 |
| 5.2.3.Stability,Precision and Peproducibility of Experiment | 第111页 |
| 5.2.4.Eco-environmental Effects on Bioconstituents of CHT | 第111-113页 |
| 5.3.Chemical Analysis of Genus Clematis and Chemotaxonomy | 第113-119页 |
| 5.3.1.Identification of the Saponins | 第114-116页 |
| 5.3.2.Presence and Distribution of Saponins in Clematis Species | 第116-119页 |
| 5.4.Ethnobotanical Survey of TMSBR | 第119-134页 |
| 5.5.Chemical Analysis of Houttuynia cordata and Its QC | 第134-144页 |
| 5.5.1.Optimum Conditions for Methylation | 第135-140页 |
| 5.5.2.QuantitativeAnalysis | 第140-143页 |
| 5.5.3.Repeatability and Recovery | 第143-144页 |
| 5.6.Results of Proteomic Analysis | 第144-174页 |
| 5.6.1.Protocol Evaluation for Leaf Proteome Analysis of Genus Clematis Taxa | 第144-153页 |
| 5.6.2.Preliminary Application of Proteomic Technique as Classification Tool for Genus Clematis | 第153-157页 |
| 5.6.3.Proteins as Bio-markers for Differentiation at Subsection Level(C.chinensis and Its Allies) | 第157-170页 |
| 5.6.4.Proteomic Approach for Inter-genera Differentiation and Classification | 第170-174页 |
| Chapter 6.DISCUSSION | 第174-195页 |
| 6.1.Phylogenetic Studies of Genus Clematis Based on Morphological Characters | 第174-175页 |
| 6.2.Chemical Analysis of C.huchouensis by HPLC-UV and QC | 第175-176页 |
| 6.3.Chemotaxonomic Importance of Saponins in Genus Clematis | 第176-179页 |
| 6.4.Significance of Ethnobotanical Studies | 第179-185页 |
| 6.4.1.Usage of Different Plants,Mode of Administration and Diseases Cured | 第179-182页 |
| 6.4.2.Medication Forms and Plant Parts Used | 第182-183页 |
| 6.4.3.Habitats of Medicinal Plants,Mode of Collection and Conservation Status | 第183-185页 |
| 6.5.Discussion of Chemical Analysis of HCT by GC/MS | 第185-187页 |
| 6.6.Proteomic Approach | 第187-195页 |
| 6.6.1.Proteome Protocol Optimization | 第187-188页 |
| 6.6.2.Preliminary Application of 2-DE Approach for Classification of Genus Clematis | 第188-191页 |
| 6.6.3.Identification of the Species Clematis chinensis from Allies Taxa | 第191-194页 |
| 6.6.4.Application of Proteomic Approach for Inter-genera Level Classification | 第194-195页 |
| Chapter 7.CONCLUISON | 第195-202页 |
| REFERENCES | 第202-236页 |
| LIST OF PUBLICATIONS | 第236-237页 |
