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丛枝菌根菌侵染和外源乙酰水杨酸对番茄耐盐性的诱导作用

Abstract第5-8页
摘要第9-18页
Chapter 1 Introduction and Review of Literature第18-45页
    1.1 Effect of salinity and plant resistance mechanism第18-23页
        1.1.1 Salinity and its effects on agriculture第18-19页
        1.1.2 Research progress on plant resistance to salinity第19-21页
            1.1.2.1 Effect of salinity on plant nutrient uptake第19页
            1.1.2.2 Effect of salinity on plant antioxidant enzymes第19页
            1.1.2.3 Effect of salinity induced plant gene expression第19-21页
        1.1.3 Mechanism of injurious effects of salinity第21-22页
        1.1.4 Research progress on tomato salinity stress resistance第22-23页
            1.1.4.1 Effect of salinity on tomato seed germination第22页
            1.1.4.2 Effect of salinity on vegetative growth of tomato第22页
            1.1.4.3 Effect of salinity on tomato yield第22-23页
    1.2 Arbuscular mycorrhizal fungi (AMF) and its application in induction of plant resistance tosalinity stress第23-34页
        1.2.1 Effect of salinity on AMF proliferation第23-24页
        1.2.2 Role of AMF in plant salinity resistance第24-34页
            1.2.2.1 Effect of AMF on vegetative growth of plants under salinity stress第25-26页
            1.2.2.2 Effect of AMF on the root morphology of salinity stressed plants第26-27页
            1.2.2.3 Effect of AMF on chlorophyll contents and photosynthesis in salinity stressed plants第27-28页
            1.2.2.4 Effect of AMF inoculation on the antioxidant defense system under salinity第28-30页
            1.2.2.5 Effect of AMF inoculation on nutrient acquisition and ionic balance uder salinity stress第30-32页
            1.2.2.6 Effect of AMF on plant gene activation in response to salinity stress第32-34页
    1.3 Role of salicylic acid (SA) in induction of plant salinity stress resistance第34-42页
        1.3.1 Effect of SA on salinity stress resistance in plants第34-42页
            1.3.1.1 Effect of SA on seed germination under salinity stress第34-35页
            1.3.1.2 Effect of SA on plant survival under salinity stress第35页
            1.3.1.3 Effect of SA on plant growth under salinity stress第35-37页
            1.3.1.4 Effect of SA on leaf area of salinity stressed plants第37页
            1.3.1.5 Effect of SA on membrane stability in salinity stressed plants第37-38页
            1.3.1.6 Effect of SA on plant physiology under salinity stress第38-39页
            1.3.1.7 Effect of SA on plant organic osmolytes under salinity stress第39页
            1.3.1.8 Effect of SA on plant chlorophyll contents and photosynthesis under salinity stress第39页
            1.3.1.9 Effect of SA on plant ion accumulation under salinity stress第39-40页
            1.3.1.10 Effect of SA on plant anti-oxidant enzymes under salinity stress第40-41页
            1.3.1.11 Effect of SA induced plant gene activation under salinity第41-42页
            1.3.1.12 Effect of SA on yield improvement under salinity stress第42页
        1.3.2 Mechanism of SA induced salinity tolerance in plants第42页
    1.4 Synergistic effect of AMF and chemical ameliorators against salinity第42-43页
    1.5 The scientific question and objectives of this study第43-45页
Chapter 2 Effect of acetyl salicylic acid and Glomus etunicatum on tomato seed germination undersalinity stress第45-55页
    Chapter Abstract第45页
    2.1 Introduction第45-46页
    2.2 Materials and Methods第46-48页
        2.2.1 Preparation of materials第47页
        2.2.2 Tomato seeds and treatments第47页
        2.2.3 Data collection and Statitical analysis第47-48页
    2.3 Results第48-52页
        2.3.1 Effects of ASA and AMF on tomato seed germination under salinity stress in plastic trays(Peat moss medium)第48-50页
            2.3.1.1 Seed germination第48页
            2.3.1.2 Fresh biomass produced第48页
            2.3.1.3 Stem length第48-49页
            2.3.1.4 Root length第49-50页
        2.3.2 Effects of ASA and AMF on tomato seed germination under salinity stress in petri plates onwet filter paper第50-52页
            2.3.2.1 Seed germination第50页
            2.3.2.2 Fresh weight第50-51页
            2.3.2.3 Stem Length第51页
            2.3.2.4 Root length and number of sub branches第51-52页
    2.4 Discussion and summary第52-55页
        2.4.1 Discussion第52-54页
        2.4.2 Summary第54-55页
Chapter 3 Effect of Acetyl salicylic acid and Glomus etunicatum root colonization on growth andestablishment of tomato seedlings under different levels of salinity stress第55-70页
    Chapter Abstract第55页
    3.1 Introduction第55-56页
    3.2 Materials and Methods第56-59页
        3.2.1 Growing of tomato seedlings and AMF root inoculation第56-57页
        3.2.2 Application of acetyl salicylic acid (ASA)第57页
        3.2.3 Treatments and salinity stress第57页
        3.2.4 Measurement of tomato plant parameters第57-59页
            3.2.4.1 Seedling survival第57页
            3.2.4.2 Growth data第57页
            3.2.4.3 Chlorophyll contents第57-58页
            3.2.4.4 Cell membrane thermo-stability第58页
            3.2.4.5 Root activity第58-59页
        3.2.5 Data analysis第59页
    3.3 Results第59-66页
        3.3.1 Seedling survival第59-60页
        3.3.2 Seedling height第60页
        3.3.3 Stem thickness第60页
        3.3.4 Leaf count第60页
        3.3.5 Leaf length第60页
        3.3.6 No. of leaflets第60-61页
        3.3.7 Seedling fresh weight第61-63页
        3.3.8 Membrane injury第63页
        3.3.9 Chlorophyll contents第63-65页
        3.3.10 Root activity第65-66页
    3.4 Discussion and Summary第66-70页
        3.4.1 Discussion第66-68页
        3.4.2 Summary第68-70页
Chapter 4 Glomus etunicatum Root Inoculation and Foliar Application of Acetyl Salicylic Acid Induced Na Cl Tolerance by up regulation of Gene Expression and Improved PhotosyntheticPerformance in Tomato Seedlings第70-87页
    Chapter Abstract第70页
    4.1 Introduction第70-71页
    4.2 Materials and Methods第71-74页
        4.2.1 Sterilization of materials and growing seedlings第71-72页
        4.2.2 Root Inoculation with AMF第72页
        4.2.3 Acetyl salicylic acid treatment and salinity stress第72页
        4.2.4 Plant measurement and data collection第72-74页
            4.2.4.1 Vegetative growth of the plants第72页
            4.2.4.2 Root development第72-73页
            4.2.4.3 Photosynthesis and chlorophyll contents第73页
            4.2.4.4 Gene expression第73-74页
        4.2.5 Statitical analysis第74页
    4.3 Results第74-82页
        4.3.1 Shoot growth第74页
        4.3.2 Effects of AMF and ASA on root architecture of tomato under salt stress第74-77页
        4.3.3 Effects of AMF and ASA on flower of tomato under salt stress第77页
        4.3.4 Biomass produced第77-78页
        4.3.5 Effects of AMF and ASA on chlorophyll contents of tomato under salt stress第78-79页
        4.3.6 Effects of AMF and ASA on photosynthetic performance of tomato under salt stress第79-80页
        4.3.7 Effects of AMF and ASA on gene expression of tomato under salt stress第80-82页
            4.3.7.1 Expression of NAC1 gene第80页
            4.3.7.2 Expression of Le NHX1 gene第80页
            4.3.7.3 Expression of JERF3 gene第80-81页
            4.3.7.4 Expression of SOS1 gene第81-82页
    4.4 Discussion and summary第82-87页
        4.4.1 Discussion第82-85页
        4.4.2 Summary第85-87页
Chapter 5 Synergistic and Individual Effect of Glomus etunicatum Root Colonization and AcetylSalicylic Acid on Tomato Plant Performance in Plastic Tunnel under Moderate salinity Stress第87-115页
    Chapter Abstract第87页
    5.1 Introduction第87-89页
    5.2 Materials and Methods第89-94页
        5.2.1 Tomato seedling preparation第89页
        5.2.2 Tomato root inoculation of AMF第89-90页
        5.2.3 Salinity stress and ASA treatment第90-91页
        5.2.4 Measurement of tomato plant parameters第91-94页
            5.2.4.1 Root fresh weight第91页
            5.2.4.2 Root architecture第91页
            5.2.4.3 Root activity第91-92页
            5.2.4.4 Chlorophyll contents第92页
            5.2.4.5 Plant enzyme activity第92页
            5.2.4.6 Fruit quality第92-94页
        5.2.5 Statistical analysis第94页
    5.3 Results第94-111页
        5.3.1 Effects of AMF and ASA on vegetative growth of tomato seedlings under salt stress第94-96页
            5.3.1.1 Leaf area and salt injury第94-95页
            5.3.1.2 Root biomass and architecture第95页
            5.3.1.3 Root length and volume第95页
            5.3.1.4 Root diameter and surface area第95-96页
            5.3.1.5 Number of nodes and tips of roots第96页
            5.3.1.6 Number of root connections and bifurcations第96页
        5.3.2 Effects of AMF and ASA on physiological parameters of tomato under salt stress第96-111页
            5.3.2.1 Antioxidant enzymes第98-100页
                5.3.2.1.1 Peroxidase (POD) activity第98页
                5.3.2.1.2 Superoxide dismutase (SOD) activity第98页
                5.3.2.1.3 Catalase (CAT) activy第98-99页
                5.3.2.1.4 Phenylalanine ammonia-lyase (PAL) activity第99-100页
            5.3.2.2 Proline contents第100页
            5.3.2.3 Chlorophyll contents第100-101页
            5.3.2.4 Effects of AMF and ASA on ion absorption of tomato under salt stress第101-106页
                5.3.2.4.1 Nitrogen contents第101-102页
                5.3.2.4.2 Phosphorous contents第102-103页
                5.3.2.4.3 Potassium contents第103页
                5.3.2.4.4 Sodium contents第103-104页
                5.3.2.4.5 Chloride contents第104-105页
                5.3.2.4.6 Na+/K+ Ratio第105-106页
            5.3.2.5 Root activity第106-107页
            5.3.2.6 Effects of AMF and ASA on yield of tomato under salt stress第107-108页
                5.3.2.6.1 Yield in July第107页
                5.3.2.6.2 Yield in August第107-108页
            5.3.2.7 Effects of AMF and ASA on fruit quality of tomato under salt stress第108-111页
                5.3.2.7.1 Lycopene contents第108-109页
                5.3.2.7.2 Juice acidity, TSS and p H第109-110页
                5.3.2.7.3 Vitamin C第110页
                5.3.2.7.4 Total Sugars第110页
                5.3.2.7.5 Protein contents第110-111页
    5.4 Discussion and summary第111-115页
        5.4.1 Discussion第111-113页
        5.4.2 Summary第113-115页
Chapter 6 Conclusion and Innovation第115-117页
    6.1 Conclusion第115-116页
    6.2 Innovation第116-117页
REFERENCES第117-137页
ACKNOWLEDGEMENT第137-139页
ABOUT THE AUTHOR第139页

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