| 中文摘要 | 第5-6页 |
| Abstract | 第6-7页 |
| Acronyms | 第8-11页 |
| CHAPTER 1:INTRODUCTION | 第11-22页 |
| 1.1 Background | 第11-19页 |
| 1.1.1 A general overview of the functionalities of Glucocorticoid and Endocannabinoid Systems as well as the implication of their relationship physiologically | 第13-15页 |
| 1.1.2 Obesity,endocannabinoids and glucocorticoids:A synopsis in brief | 第15-17页 |
| 1.1.3 The HPLC-MS/MS analysis Technique | 第17-19页 |
| 1.2 Statement of the problem | 第19页 |
| 1.3 Significance of the study | 第19-20页 |
| 1.4 Research objective | 第20-22页 |
| 1.4.1 General objective | 第20页 |
| 1.4.2 Specific objectives | 第20-22页 |
| CHAPTER 2:REVIEW OF RELATED LITERATURE | 第22-37页 |
| 2.1 An outline of the detection methods for endocannabinoids and glucocorticoids in variousmatrices | 第23-25页 |
| 2.2 Matrix effect in LC-MS/MS based method of analyses and the importance of solid phaseextraction application | 第25-28页 |
| 2.3 The peculiar chemistry and stability of 2-AG and AEA | 第28-31页 |
| 2.4 Stress,obesity,endocannabinoids and glucocorticoids:A view at their relationship and itsclinical implications | 第31-35页 |
| 2.5 Merits and demerits associated with quantification of analytes in hair | 第35-37页 |
| CHAPTER 3:RESEARCH METHODOLGY | 第37-46页 |
| 3.1 Reagents and solution preparation | 第37-38页 |
| 3.2 Research Participants | 第38页 |
| 3.3 Hair sample collection | 第38页 |
| 3.4 Sample treatment | 第38-39页 |
| 3.5 HPLC-MS/MS conditions | 第39-43页 |
| 3.6 Method validation | 第43-45页 |
| 3.7 Statistical Analysis | 第45-46页 |
| CHAPTER 4:RESEARCH RESULTS | 第46-52页 |
| 4.1 Chromatography | 第46-47页 |
| 4.2 Validation parameters | 第47-49页 |
| 4.3 Intercorrelations between AEA,2-AG,cortisol and cortisone | 第49-51页 |
| 4.4 The influence of gender and BMI | 第51-52页 |
| CHAPTER 5:DISCUSSION OF RESEARCH FINDINGS | 第52-59页 |
| CHAPTER 6:LIMITATIONS OF THE STUDY, RECOMMENDATIONS FOR FUTURE RESEARCH AND CONCLUSION | 第59-61页 |
| ACKNOWLEDGEMENTS | 第61-62页 |
| REFERENCES | 第62-71页 |
