| 摘要 | 第7-9页 |
| ABSTRACT | 第9-11页 |
| Chapter 1 Review of Literature | 第15-22页 |
| 1.1 INTRODUCTION TO GLOBAL WHEAT PRODUCTION AND THE CHALLENGES | 第15-16页 |
| 1.1.1 Climate effects on wheat yields | 第15页 |
| 1.1.2 Effect of drought on plant growth | 第15-16页 |
| 1.2 IMPROVING WHEAT YIELDS | 第16-19页 |
| 1.2.1 Photosynthesis | 第16-17页 |
| 1.2.2 Impact of drought stress on photosynthesis | 第17页 |
| 1.2.3 Rubisco and photosynthesis | 第17页 |
| 1.2.4 Rubisco activase (Rca) | 第17-18页 |
| 1.2.5 Rubisco activase isoforms | 第18-19页 |
| 1.3 ASSOCIATION MAPPING AND APPLICATIONS | 第19-20页 |
| 1.3.1 Association mapping withmolecular markers | 第19-20页 |
| 1.3.2 Association mapping with candidate genes | 第20页 |
| 1.4 MAIN RESEARCH WORKS AND OUTLINE OF EXPERIMENTAL DESIGN | 第20-21页 |
| 1.4.1 Main research works | 第20-21页 |
| 1.4.2 Outline of experimental design | 第21页 |
| 1.5 Objectives of the study | 第21-22页 |
| Chapter 2 Association mapping for Pn, and yield traits under two moisture conditions and their drought tolerance indices in winter bread wheat (Triticum aestivem L.) with SSR markers | 第22-53页 |
| 2.1 INTRODUCTION | 第22-23页 |
| 2.2 MATERIALS AND METHODS | 第23-27页 |
| 2.2.1 Plant material and growth conditions | 第23-24页 |
| 2.2.2 Genotyping with SSR markers | 第24-25页 |
| 2.2.3 Net photosynthesis rate (Pn) (μmol m-2 s-1) | 第25页 |
| 2.2.4 Measurement of agronomic traits | 第25页 |
| 2.2.5 Data analysis | 第25-27页 |
| 2.3 RESULTS | 第27-46页 |
| 2.3.1 Variations of phenotypic traits among wheat genotypes | 第27-29页 |
| 2.3.2 Variations for drought tolerance indices among wheat genotypes | 第29-31页 |
| 2.3.3 Genetic diversityamong wheat genotypes and population structure | 第31-34页 |
| 2.3.4 Marker-trait Associations | 第34-40页 |
| 2.3.5 Marker-trait associations for drought tolerance indices | 第40-46页 |
| 2.4 DISCUSSION | 第46-52页 |
| 2.4.1 GENETIC DIVERSITY, LINKAGE DISEQUILIBRIUM AND POPULATION STRUCTURE | 第46-48页 |
| 2.4.2 MARKER-TRAIT ASSOCIATIONS FOR PHOTOSYNTHESIS RELATED TRAITS | 第48-51页 |
| 2.4.3 Marker-trait associations for drought tolerance indices | 第51页 |
| 2.4.4 Marker-trait associations across environments and across seasons | 第51-52页 |
| 2.5 CONCLUSIONS | 第52-53页 |
| Chapter 3 The expression of TaRca2-α gene associated with Pn, biomass and grain yieldin bread wheat (Triticumaestivum L.) under field condition | 第53-82页 |
| 3.1 INTRODUCTION | 第53-54页 |
| 3.2 MATERIALS AND METHODS | 第54-58页 |
| 3.2.1 Plant material and growth conditions | 第54页 |
| 3.2.2 Candidate gene analysis | 第54-56页 |
| 3.2.3 Rubisco activase activity in leaf extracts | 第56-57页 |
| 3.2.4 Phenotypic evaluation | 第57-58页 |
| 3.2.5 Data analysis | 第58页 |
| 3.3 RESULTS | 第58-76页 |
| 3.3.1 Sequence search and analysis | 第58-60页 |
| 3.3.2 Confirmation of genome-specificity of the primer pairs | 第60-61页 |
| 3.3.3 Expression of TaRca2-α copies in bread wheat | 第61-62页 |
| 3.3.4 Rubisco activase activity in flag leaf of 59 bread wheat genotypes | 第62-64页 |
| 3.3.5 Clustering of wheat genotypes based on TaRca2-α expression | 第64-69页 |
| 3.3.6 Correlation between TaRca2-α copies expression and Pn | 第69-71页 |
| 3.3.7 Correlation between TaRca2-α copies expression with BMPP and GYPP | 第71-74页 |
| 3.3.8 Correlation between Rubisco activase activity with BMPP and GYPP | 第74-76页 |
| 3.4 DISCUSSION | 第76-81页 |
| 3.4.1 Expression patterns of TaRca2-α in wheat | 第76-78页 |
| 3.4.2 Correlation of TaRca2-α copies’ expression with Pn | 第78页 |
| 3.4.3 Correlation of TaRca2-α copies’ expression with BMPP | 第78-79页 |
| 3.4.4 Correlation of TaRca2-α copies’ expression with GYPP | 第79-80页 |
| 3.4.5 Correlation of TaRca2-α expression with Rubisco activase activity | 第80-81页 |
| 3.5 CONCLUSIONS | 第81-82页 |
| Chapter4 General Conclusions | 第82-85页 |
| 4.1 VARIABILITY FOR PHENOTYPIC TRAITS IN THE TEST PANEL | 第82页 |
| 4.2 MARKER-TRAIT ASSOCIATION ANALYSES | 第82-83页 |
| 4.3 CANDIDATE GENE ANALYSIS AND ENZYME ACTIVITY | 第83-84页 |
| Further works | 第84-85页 |
| References | 第85-97页 |
| Appendix 1 | 第97-99页 |
| Appendix 2 | 第99-101页 |
| ACKNOWLEDGEMENTS | 第101-102页 |
| CURRICULUM VITAE | 第102页 |
