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Developing Gene-Tagged Molecular Markers for Evaluation of Genetic Association of Apple V-ATPase Genes with Fruit Acidity

摘要第6-7页
ABSTRACT第7-8页
1. INTRODUCTION第11-15页
2. LITERATURE REVIEW第15-23页
    2.1 Research Background第15-16页
    2.2 Malic and Citric Acid Biosynthesis第16-17页
    2.3 Organic acid transporters and degradation第17-20页
    2.4 Vacuolar H+- ATPases (VHA)第20-23页
        2.4.1 V- ATPase structure第20-23页
3. MATERIALS AND METHODS第23-29页
    3.1 Plant material第23页
    3.2 Measurement of malic acid content through HPLC第23-24页
    3.3 Apple Genomic DNA extraction第24页
    3.4 Identification of H+- ATPase genes in apple第24-25页
    3.5 RNA isolation and quantitative real-time(qRT- PCR)第25-27页
    3.6 Development of gene- tagged SSR markers第27-28页
    3.7 Statistical analysis第28-29页
4. RESULTS第29-35页
    4.1 Identification of VHA genes in apple genome第29-31页
    4.2 Expression profiling of VHA genes in apple fruit at differentdevelopmental stages第31-32页
    4.3 Gene-tagged marker development for VHA genes with high malicacid expression profiles in fruit and their associated polymorphismsin a collection of apple accessions第32-33页
    4.4 Polymorphic VHA genes and apple fruit malic acid accumulation第33-35页
5. DISCUSSION第35-38页
    5.1 Duplication of VHA genes in apple第35-36页
    5.2 Potential VHA gene associated with malic acid accumulation inapple fruit第36-38页
6. CONCLUSION第38-39页
REFERENCES第39-45页
Publications第45-46页
APPENDIX A第46-47页
APPENDIX B第47-48页
APPENDIX C第48-49页
ACKNOWLEDGEMENT第49页

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