| 作者简介 | 第7-9页 |
| 摘要 | 第9-14页 |
| ABSTRACT | 第14-18页 |
| Chapter 1 Literature Review | 第21-31页 |
| 1.1 Introduction | 第21-22页 |
| 1.2 Previous studies | 第22-28页 |
| 1.2.1 Geogenic arsenic | 第22页 |
| 1.2.2 Distribution and characteristics of high arsenic groundwater | 第22-23页 |
| 1.2.3 Arsenic enrichment and mobilization mechanism in natural aquifers | 第23页 |
| 1.2.4 Microbially mediated arsenic release | 第23-26页 |
| 1.2.5 Research status of high arsenic groundwater in Hetao Plain | 第26-27页 |
| 1.2.6 Application of microbial molecular ecology | 第27-28页 |
| 1.3 Research objectives | 第28-29页 |
| 1.4 Organization of the thesis | 第29-31页 |
| Chapter 2 Geochemical characteristics of high arsenic aquifers | 第31-40页 |
| §2.1 Introduction | 第31-32页 |
| §2.2 Material and methods | 第32-34页 |
| 2.2.1 Site description | 第32页 |
| 2.2.2 Sediment sample collection and geochemical analysis | 第32-33页 |
| 2.2.3 Groundwater sampling and geochemical analysis | 第33-34页 |
| §2.3 Results and discussion | 第34-39页 |
| 2.3.1 Sediment geochemistry | 第34-36页 |
| 2.3.2 Groundwater geochemistry | 第36-39页 |
| §2.4 Summary | 第39-40页 |
| Chapter 3 Microbial diversity and community structure in high arsenic aquifers | 第40-60页 |
| §3.1 Introduction | 第40-41页 |
| §3.2 Material and methods | 第41-43页 |
| 3.2.1 DNA extraction and PCR-DGGE analysis | 第41-42页 |
| 3.2.2 The 16S rRNA gene clone library construction,sequencing and phylogenetic analyses | 第42-43页 |
| 3.2.3 Nucleotide sequence accession numbers | 第43页 |
| §3.3 Results and discussion | 第43-59页 |
| 3.3.1 Bacterial diversity and community structure in high arsenic sediments | 第43-49页 |
| 3.3.2 Microbial diversity in high arsenic groundwater | 第49-57页 |
| 3.3.3 Different bacterial communities in sediments and groundwater | 第57-59页 |
| §3.4 Summary | 第59-60页 |
| Chapter 4 Variations of microbial communities in groundwaters with different arsenic concentrations | 第60-72页 |
| §4.1 Introduction | 第60-61页 |
| §4.2 Material and methods | 第61-63页 |
| 4.2.1 Site description and sample collection | 第61-62页 |
| 4.2.2 PCR amplification and Illumina sequencing | 第62页 |
| 4.2.3 Sequences analysis | 第62-63页 |
| 4.2.4 Statistical analysis of geochemical and microbial data | 第63页 |
| §4.3 Results and discussion | 第63-70页 |
| 4.3.1 Groundwater geochemistry | 第63-64页 |
| 4.3.2 Community composition analysis | 第64-66页 |
| 4.3.3 Diversity indices | 第66-68页 |
| 4.3.4 Statistical analysis | 第68-70页 |
| §4.4 Summary | 第70-72页 |
| Chapter 5 Functional microbial communities in strongly reducing high arsenic groundwater | 第72-93页 |
| §5.1 Introduction | 第72-73页 |
| §5.2 Material and methods | 第73-78页 |
| 5.2.1 DNA extraction and functional gene PCR amplification | 第73-75页 |
| 5.2.2 dsrB gene DGGE analysis | 第75页 |
| 5.2.3 Clone library construction and phylogenetic analysis | 第75-76页 |
| 5.2.4 Nucleotide sequence accession numbers | 第76页 |
| 5.2.5 Quantitative PCR | 第76-78页 |
| §5.3 Results and discussion | 第78-92页 |
| 5.3.1 Sulfate-reducing bacteria | 第78-82页 |
| 5.3.2 Methanogen Archaea | 第82-86页 |
| 5.3.3 Arsenic reducing and oxidizing bacteria | 第86-92页 |
| §5.4 Summary | 第92-93页 |
| Chapter 6 Conclusions and suggestions for future research work | 第93-95页 |
| §6.1 Conclusions | 第93-94页 |
| §6.2 Suggestions for future research work | 第94-95页 |
| Acknowledgement | 第95-96页 |
| References | 第96-107页 |
