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河套平原强还原高砷地下水系统微生物分子生态学研究

作者简介第7-9页
摘要第9-14页
ABSTRACT第14-18页
Chapter 1 Literature Review第21-31页
    1.1 Introduction第21-22页
    1.2 Previous studies第22-28页
        1.2.1 Geogenic arsenic第22页
        1.2.2 Distribution and characteristics of high arsenic groundwater第22-23页
        1.2.3 Arsenic enrichment and mobilization mechanism in natural aquifers第23页
        1.2.4 Microbially mediated arsenic release第23-26页
        1.2.5 Research status of high arsenic groundwater in Hetao Plain第26-27页
        1.2.6 Application of microbial molecular ecology第27-28页
    1.3 Research objectives第28-29页
    1.4 Organization of the thesis第29-31页
Chapter 2 Geochemical characteristics of high arsenic aquifers第31-40页
    §2.1 Introduction第31-32页
    §2.2 Material and methods第32-34页
        2.2.1 Site description第32页
        2.2.2 Sediment sample collection and geochemical analysis第32-33页
        2.2.3 Groundwater sampling and geochemical analysis第33-34页
    §2.3 Results and discussion第34-39页
        2.3.1 Sediment geochemistry第34-36页
        2.3.2 Groundwater geochemistry第36-39页
    §2.4 Summary第39-40页
Chapter 3 Microbial diversity and community structure in high arsenic aquifers第40-60页
    §3.1 Introduction第40-41页
    §3.2 Material and methods第41-43页
        3.2.1 DNA extraction and PCR-DGGE analysis第41-42页
        3.2.2 The 16S rRNA gene clone library construction,sequencing and phylogenetic analyses第42-43页
        3.2.3 Nucleotide sequence accession numbers第43页
    §3.3 Results and discussion第43-59页
        3.3.1 Bacterial diversity and community structure in high arsenic sediments第43-49页
        3.3.2 Microbial diversity in high arsenic groundwater第49-57页
        3.3.3 Different bacterial communities in sediments and groundwater第57-59页
    §3.4 Summary第59-60页
Chapter 4 Variations of microbial communities in groundwaters with different arsenic concentrations第60-72页
    §4.1 Introduction第60-61页
    §4.2 Material and methods第61-63页
        4.2.1 Site description and sample collection第61-62页
        4.2.2 PCR amplification and Illumina sequencing第62页
        4.2.3 Sequences analysis第62-63页
        4.2.4 Statistical analysis of geochemical and microbial data第63页
    §4.3 Results and discussion第63-70页
        4.3.1 Groundwater geochemistry第63-64页
        4.3.2 Community composition analysis第64-66页
        4.3.3 Diversity indices第66-68页
        4.3.4 Statistical analysis第68-70页
    §4.4 Summary第70-72页
Chapter 5 Functional microbial communities in strongly reducing high arsenic groundwater第72-93页
    §5.1 Introduction第72-73页
    §5.2 Material and methods第73-78页
        5.2.1 DNA extraction and functional gene PCR amplification第73-75页
        5.2.2 dsrB gene DGGE analysis第75页
        5.2.3 Clone library construction and phylogenetic analysis第75-76页
        5.2.4 Nucleotide sequence accession numbers第76页
        5.2.5 Quantitative PCR第76-78页
    §5.3 Results and discussion第78-92页
        5.3.1 Sulfate-reducing bacteria第78-82页
        5.3.2 Methanogen Archaea第82-86页
        5.3.3 Arsenic reducing and oxidizing bacteria第86-92页
    §5.4 Summary第92-93页
Chapter 6 Conclusions and suggestions for future research work第93-95页
    §6.1 Conclusions第93-94页
    §6.2 Suggestions for future research work第94-95页
Acknowledgement第95-96页
References第96-107页

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