| 摘要 | 第4-5页 |
| Abstract | 第5-6页 |
| CHAPTER ONE INTRODUCTION | 第13-21页 |
| 1.1 Background and motivation | 第13页 |
| 1.2 Literature Review | 第13-17页 |
| 1.2.1 Pretreatment of lignocellulosic biomass | 第14-16页 |
| 1.2.2 Immobilization of yeast cells | 第16页 |
| 1.2.3 Response surface methodology | 第16-17页 |
| 1.3 Existing Problem | 第17页 |
| 1.4 Aims | 第17页 |
| 1.5 Objectives | 第17-21页 |
| CHAPTER TWO Microwave-Alkali Based Pretreatment of Lignocellulosic Biomass | 第21-35页 |
| 2.1 Introduction | 第21页 |
| 2.2 Materials and Methods | 第21-24页 |
| 2.2.1 Raw material | 第21-22页 |
| 2.2.2 The feasibility of the microwave-assisted alkali pretreatment in lignocellulosic biomass | 第22页 |
| 2.2.3 Optimization of pretreatment conditions | 第22页 |
| 2.2.4 RS and SB chemical composition and sugar analysis | 第22-23页 |
| 2.2.5 Enzymatic hydrolysis | 第23-24页 |
| 2.3 Results and discussion | 第24-33页 |
| 2.3.1 The feasibility of the microwave-assisted alkali pretreatment in lignocellulosic biomass | 第24-26页 |
| 2.3.2 Optimization of pretreatment condition | 第26-31页 |
| 2.3.3 Enzyme hydrolysis of pretreated lignocellulosic biomasses | 第31页 |
| 2.3.4 Optimization of enzyme loading | 第31-33页 |
| 2.4 Conclusion | 第33-35页 |
| CHAPTER THREE Cellulase Enzyme Production by Aspergillus niger | 第35-49页 |
| 3.1 Introduction | 第35页 |
| 3.2 Materials and Method | 第35-40页 |
| 3.2.1 Substrate and pretreatment | 第35-36页 |
| 3.2.2 Mictoorganism and culture medium | 第36页 |
| 3.2.3 Fermentation and Cellulase-system production | 第36-37页 |
| 3.2.4 Nutritional factors study | 第37-38页 |
| 3.2.5 Enzyme Production | 第38页 |
| 3.2.6 Analytical methods | 第38-40页 |
| 3.3 Results and Discussion | 第40-47页 |
| 3.3.1 Effect of different fermentation methods on cellulase production | 第41-44页 |
| 3.3.2 Influence of Wheat Bran addition on Cellulase Production | 第44页 |
| 3.3.3 Study on effect of nutrient-limited system | 第44-45页 |
| 3.3.4 Characterization of crude cellulase enzyme | 第45-47页 |
| 3.4 Conclusion | 第47-49页 |
| CHAPTER FOUR Immobilized and Free Yeast Cell Fermentation | 第49-59页 |
| 4.1 Introduction | 第49页 |
| 4.2 Material and Methods | 第49-53页 |
| 4.2.1 Feedstock preparation and pretreatment procedures | 第49页 |
| 4.2.2 Microwave-alkali assisted pretreatment | 第49-50页 |
| 4.2.3 Microorganism and Inoculum preparation | 第50页 |
| 4.2.4 Yeast immobilization | 第50-51页 |
| 4.2.5 Microencapsulation efficiency | 第51页 |
| 4.2.6 Microcapsule size | 第51页 |
| 4.2.7 Estimation of Immobilized Cells | 第51-52页 |
| 4.2.8 Bioethanol Fermentation by Delignified Cellulosic Material Supported Biocatalyst | 第52页 |
| 4.2.9 Repeated Batch Fermentations for ethanol production | 第52-53页 |
| 4.3 Results and Discussion | 第53-58页 |
| 4.3.1 Morphology of Saccharomyces cerevisiae in solid medium and its growth curve | 第53-54页 |
| 4.3.2 Microencapsulation establishment efficiency | 第54-55页 |
| 4.3.3 Microcapsule size analysis | 第55页 |
| 4.3.4 Bioethanol production by immobilized yeast in a batch system | 第55-57页 |
| 4.3.5 The application of immobilized yeast in multiple batch fermentation cycles | 第57-58页 |
| 4.4 Conclusion | 第58-59页 |
| CHAPTER FIVE RESPONSE SURFACE METHODOLOGY | 第59-75页 |
| 5.1 Introduction | 第59页 |
| 5.2 Material and Methods | 第59-60页 |
| 5.2.1 Experimental design for cellulase production and enzymatic hydrolysis | 第59-60页 |
| 5.2.2 Experimental design for ethanol production optimization | 第60页 |
| 5.2.3 Validation of the experimental model | 第60页 |
| 5.3 Results and Discussion | 第60-72页 |
| 5.3.1 Optimization of Cellulase Production | 第60-64页 |
| 5.3.2 Optimization of Enzymatic saccharification | 第64-68页 |
| 5.3.3 Validation of Models | 第68-69页 |
| 5.3.4 Optimization of Bioethanol Production | 第69-72页 |
| 5.4 Conclusion | 第72-75页 |
| CHAPTER SIX CONCLUSIONS AND SUGGESTIONS | 第75-81页 |
| 6.1 Conclusion | 第75-77页 |
| 6.1.1 Microwave-Alkali Based Pretreatment of Lignocellulosic Biomass for Bioethanol Production | 第75-76页 |
| 6.1.2 Cellulase Enzyme Production by Aspergillus niger | 第76页 |
| 6.1.3 Immobilized and Free Yeast Cell Fermentation | 第76页 |
| 6.1.4 A statistical optimization-Response Surface Methodology | 第76-77页 |
| 6.2 Suggestions | 第77-81页 |
| Acknowledgement | 第81-83页 |
| References | 第83-89页 |
| Publications | 第89-90页 |
| Nomenclature | 第90-91页 |
| Curriculum Vitae | 第91页 |
