| 中文摘要 | 第1-8页 |
| Abstract | 第8-10页 |
| 1 Literature Review | 第10-22页 |
| ·Introduction | 第10-12页 |
| ·Heavy-Metals as contaminants | 第12-13页 |
| ·Phytoremediation: A suitable approach to clean up pollution | 第13-19页 |
| ·Mechanism and properties of phytoremediation | 第13-15页 |
| ·Transgenic plants for phytoremediation of heavy-metals | 第15-19页 |
| ·Obstacles in the application of transgenic plants in phytoremediation | 第19-20页 |
| ·Contents and meaning of this study | 第20-22页 |
| ·Research contents | 第20-21页 |
| ·Significance of the research | 第21-22页 |
| 2 Materials and Methods | 第22-36页 |
| ·Experimental materials | 第22-23页 |
| ·Tobacco plant | 第22页 |
| ·Bacteria and plasmids | 第22页 |
| ·The main reagents | 第22页 |
| ·The main equipments | 第22页 |
| ·Culture mediums | 第22-23页 |
| ·Experimental methods | 第23-36页 |
| ·Separation of target gene | 第23页 |
| ·Construction of eukaryotic expression vector | 第23-24页 |
| ·Agrobacterium tumefacien mediated target genes transduction into tobacco | 第24-25页 |
| ·Cultivation of transgenic tobacco and detection of target genes | 第25-27页 |
| ·Semi-quantitative analysis of transgenic tobacco plant | 第27-30页 |
| ·Determination of physiological indexes of transgenic tobacco plant | 第30-34页 |
| ·Determination of metal content | 第34-36页 |
| 3 Results and analysis | 第36-52页 |
| ·Construction of plant expression vectors | 第36-37页 |
| ·Transduction of plant expression vectors into Agrobacterium tumefaciens | 第37-38页 |
| ·Agrobacterium tumefacien mediated target gene transduction into tobacco | 第38-40页 |
| ·Cultivation of transgenic tobacco and detection of target genes | 第40-41页 |
| ·Cultivation of transgenic tobacco | 第40-41页 |
| ·Detection of target genes | 第41页 |
| ·Semi-quantitative RT-PCR analysis of transgenic tobacco | 第41-44页 |
| ·Extraction of tobacco RNA | 第41-42页 |
| ·Determination of the number of PCR cycle | 第42-44页 |
| ·Semi-quantitative RT-PCR analysis | 第44页 |
| ·Determination of metal Cd content | 第44-45页 |
| ·Determination of resistant physiological indexes of transgenic tobacco | 第45-52页 |
| ·Enzymatic activity of SOD | 第45-47页 |
| ·Enzymatic activity of POD | 第47-48页 |
| ·Content of MDA | 第48页 |
| ·Content of proline | 第48-52页 |
| 4. Discussion | 第52-58页 |
| ·Phytoremediation of Cd contamination | 第52-54页 |
| ·Conclusion of this experiment | 第54-55页 |
| ·Prospective and improvement | 第55-58页 |
| References | 第58-63页 |
| Appendixes | 第63-71页 |
| AppendixⅠThe gene information of OASA4 and γ-GCS | 第63-65页 |
| AppendixⅡ：Mediums used in this study | 第65-66页 |
| AppendixⅢ: Several experimental methods | 第66-69页 |
| AppendixⅣ: Abbreviations | 第69-71页 |
| Acknowledgement | 第71页 |
