| 摘要 | 第3-8页 |
| abstract | 第8-9页 |
| CHAPTER 1. INTRODUCTION | 第12-34页 |
| 1.1 Fluorescent probes | 第12-15页 |
| 1.2 Classification of fluorescence | 第15-26页 |
| 1.2.1 Photoinduced Electron Transfer (PET) | 第16-18页 |
| 1.2.2 Intramolecular Charge Transfer (ICT) | 第18-21页 |
| 1.2.3 F?rster Resonance Energy Transfer (FRET) | 第21-24页 |
| 1.2.4 Reduction oxidation reaction(Redox) | 第24-26页 |
| 1.3 Overview of some common fluorophores used in fluorescent probes | 第26-34页 |
| CHAPTER 2 FLUORESCENCE-BASED ASSAYS FOR DISEASE-RELATEDLIVER TRANSPORT PROTEIN | 第34-54页 |
| 2.1 Preparation of phosphine-fluorophore substrate | 第36-38页 |
| 2.2 Synthesis of naphthalimide derivatives | 第38-49页 |
| 2.2.1 Reactions involving 4-(diphenylphosphino)benzoic acid | 第41-42页 |
| 2.2.2 Reactions involving 2-(diphenylphosphino)benzoic acid | 第42-48页 |
| 2.2.3 Reactions involving tris-carboxyethylphosphine (TCEP) | 第48-49页 |
| 2.3 Synthesis of lactic acid analogue | 第49-51页 |
| 2.4 Conclusion | 第51-54页 |
| CHAPTER 3. DEVELOP STABLE HUMAN LIVER CELL AND LUMINESCENEASSAY ON CYP3A4 | 第54-62页 |
| 3.1 Developed stable human liver cell cultures with over-expressed lactatetransporters | 第54-55页 |
| 3.2 Luminescene assay on CYP3A4 of human liver microsome | 第55-59页 |
| 3.3 Fluorescent molecules for biochemistry assay | 第59-60页 |
| 3.4 Conclusion | 第60-62页 |
| CHAPTER 4. EXPERIMENTAL SECTION | 第62-82页 |
| 4.1 General | 第62-63页 |
| 4.2 Synthesis procedures | 第63-82页 |
| 4.2.1 Synthesis of naphthalimide derivatives | 第63-65页 |
| 4.2.2 Synthesis of triphenyl phosphine | 第65-67页 |
| 4.2.3 Coupling with analogs of triphenyl phosphine | 第67页 |
| 4.2.4 Reactions involving 4-(diphenylphosphino)benzoic acid | 第67页 |
| 4.2.5 Reactions involving 2-(diphenylphosphino)benzoic acid | 第67-71页 |
| 4.2.6 Reactions involving 5-nitro-1,2-dihydroacenaphthylene | 第71-72页 |
| 4.2.7 Reactions involving hydrazine hydrate | 第72-74页 |
| 4.2.8 Reactions involving TCEP | 第74-75页 |
| 4.2.9 Synthesis of lactic acid analogue | 第75-76页 |
| 4.2.10 Synthesis of Jacobsen catalyst | 第76-78页 |
| 4.2.11 Synthesis of molecules in biochemistry assay | 第78-82页 |
| REFERENCES | 第82-90页 |
| APPENDICES | 第90-104页 |
| Appendix A: NMR data | 第90-100页 |
| Appendix B: Fluorometric analysis | 第100-101页 |
| Appendix C: HPLC data analysis | 第101-102页 |
| Appendix D: GC data analysis | 第102-103页 |
| Appendix E: Data of luminescene assay in CYP3A4 of human liver | 第103-104页 |
| ACKNOWLEDGEMENTS | 第104页 |
