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Modulation of Microglia Function by Dopamine and Serotonin

ABSTRACT第4页
Chapter 1 Introduction第6-16页
    1.1 Microglia第6-12页
        1.1.1 The origin第6-7页
        1.1.2 The microglial morphology and activation第7-9页
        1.1.3 The microglial function第9-10页
        1.1.4 Microglia and disease第10-12页
    1.2 Cell signaling pathway and cell chemotaxis第12-15页
        1.2.1 MAPK cell signaling第12-14页
        1.2.2 Paxillin and cofilin第14-15页
    1.3 Hypothesis and Aims第15-16页
Chapter 2 Experimental Materials and Methods第16-30页
    2.1 Experiment materials第16-19页
        2.1.1 Cell第16页
        2.1.2 Antibody and reagent第16-18页
        2.1.3 Instrument第18-19页
    2.2 Solution preparation第19-20页
    2.3. Method第20-30页
        2.3.1 Cell culture第20页
        2.3.2 Cells subculture:第20-21页
        2.3.3 Cryopreservation of cells第21页
        2.3.4 Cells recovery:第21-22页
        2.3.5 Western blot for cellular protein levels detection:第22-26页
        2.3.6 Transformation第26-28页
        2.3.7 BCA for total protein level detection第28-30页
Chapter 3 Result第30-42页
    3.1 ERK1/2 activation was not significantly altered upon DA or 5-HT stimulation inBv2 microglia cells第30-31页
    3.2 DA and 5-HT induce the activation of p38 MAPK in a time-dependent manner inresting microglia第31-34页
    3.3 Activation of p38 MAPK by DA cannot be blocked with DA receptor antagonists.第34-36页
    3.4 Activation of p38 MAPK by DA or 5-HT can be blocked with decynium第36-38页
    3.5 Activation of p38 MAPK results in the phosphorylation of paxillin Ser83第38-40页
    3.6 Phagocytosis and F-actin stress fibers are downregulated in DA-treatedmicroglia第40-42页
Chapter 4 Discussion第42-47页
Reference第47-52页
Acknowledgement第52-53页

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