| ABSTRACT (Chinese) | 第1-7页 |
| ABSTRACT (English) | 第7-11页 |
| CONTENTS | 第11-15页 |
| CHAPTER 1 INTRODUCTION | 第15-21页 |
| CHAPTER 2 THE EFFECT OF RHYNCHOPHYLLINE ON THE ANIMAL MODEL OF METHAMPHETAMINE DEPENDENCE:CONDITIONED PLACE PREFERENCE TEST | 第21-25页 |
| ·Experimental animals | 第21页 |
| ·Drug administration and place conditioning | 第21-22页 |
| ·Conditioning scores and data analysis | 第22-23页 |
| ·Results | 第23-25页 |
| CHAPTER 3 THE EFFECT OF RHYNCHOPHYLLINE ON PHOSPHO-SPECIFIC NR1 SUBUNITS AT SERINE 897 EXPRESSION IN THE RAT BRAIN | 第25-32页 |
| ·Immunofluorescence | 第25-26页 |
| ·Control for immunofluorescence | 第26页 |
| ·Digital image analyses and statistics | 第26-27页 |
| ·Results | 第27-32页 |
| ·Control for immunofluorescence | 第27页 |
| ·Changes of phospho-NR1 serine 897 expression in the striatum (caudate putamen and nucleus accumbens) | 第27-30页 |
| ·Changes of phospho-NR1 serine 897 expression in the ventral tegmental area | 第30-32页 |
| CHAPTER 4 THE PROTECTION OF RHYNCHOPHYLLINE AGAINST METHAMPHETAMINE NEUROTOXICITY | 第32-36页 |
| ·Preparation of drugs | 第32页 |
| ·Primary cell culture | 第32-33页 |
| ·MTT assay | 第33-34页 |
| ·Results | 第34-36页 |
| ·Cell viability measurement for MA neurotoxicity | 第34页 |
| ·Protective effect of rhynchophylline on the MA treated neurons | 第34-36页 |
| CHAPTER 5 THE INHIBITION OF CALCIUM INFLUX BY RHYNCHOPHYLLINE IN METHAMPHETAMINE PRETREATED CORTICAL NEURONS | 第36-42页 |
| ·Intracellular [Ca~(2+)]_i measurement with different concentration MA exposure for 48 h | 第36页 |
| ·Time series of calcium imaging | 第36-38页 |
| ·Time course for the changes of [Ca~(2+)]_i in 48h MA-pretreated neurons | 第36-37页 |
| ·Time course for the changes of [Ca~(2+)]_i in normal neurons without MA-pretreatment | 第37-38页 |
| ·Results | 第38-42页 |
| ·Increase of [Ca~(2+)]_i in the cortical neurons with MA exposure for 48 h | 第38-39页 |
| ·Time course for the changes of [Ca~(2+)]_i in 48h MA-pretreated neurons | 第39-40页 |
| ·Time course for the changes of [Ca~(2+)]_i in normal neurons without MA-pretreatment | 第40-42页 |
| CHAPTER 6 DISCUSSION AND CONCLUSION | 第42-48页 |
| APPENDIX | 第48-95页 |
| LIST OF FIGURES | 第48-68页 |
| LIST OF TABLES | 第68-95页 |
| ABBREVIATIONS | 第95-97页 |
| REFERENCE | 第97-103页 |
| PULICATION ARISING FROM THIS THESIS | 第103-104页 |
| ACKNOWLEDGEMENTS | 第104-106页 |
| Statement of original authorship | 第106-107页 |
| 学位论文原创性声明 | 第107页 |
| 学位论文版权使用授权书 | 第107页 |
