| Abstract | 第1-9页 |
| 1. Introduction | 第9-22页 |
| ·Research Progress on the Plant Molecular Response Mechanisms Under the cold stress | 第10-15页 |
| ·Tow-dimensional difference gel electrophoresis (2D-DIGE) Technology | 第15-19页 |
| ·The Application of Differential-display 2D-DIGE Proteomics in Plant cold stress Research | 第19-22页 |
| 2. Proteomics Analysis of Early Cold Response in Arabidopsis | 第22-41页 |
| ·Materials and Methods | 第23-30页 |
| ·Plant Materials | 第23页 |
| ·Protein Sample Preparation | 第23-25页 |
| ·Two-dimensional difference ge electrophoresis (2-D DIGE) | 第25-30页 |
| ·Results and Analysis | 第30-38页 |
| ·2-D DIGE analysis of cold response in the soluble fraction of Arabidopis proteome | 第30-33页 |
| ·2-D DIGE analysis of cold response in the Sodium carbonate-soluble microsomal fraction of Arabidopis proteome | 第33-36页 |
| ·2-D DIGE analysis of cold response in the Nuclei fraction of Arabidopis proteome | 第36-38页 |
| ·Discussion | 第38-41页 |
| 3. Functional Characterization of DPE2 in Arabidopsis thaliana Cold Response | 第41-72页 |
| ·Materials and Methods | 第42-50页 |
| ·Plant materials and growth conditions | 第42页 |
| ·Time course western blot | 第42-44页 |
| ·Freezing treatment of whole plants | 第44页 |
| ·Electrolyte leakage measurement | 第44-45页 |
| ·Creating CaMV35S:YFP-DPE2 transgenic plants | 第45-47页 |
| ·Confocal laser scanning microscopy | 第47-48页 |
| ·Measurement OF DPE2 activity | 第48页 |
| ·Native PAGE gel | 第48-49页 |
| ·real-time PCR | 第49-50页 |
| ·Results and Analysis | 第50-68页 |
| ·DPE2 became less soluble after cold treatment | 第50-52页 |
| ·DPE2 knockout lines have increased freezing tolerance | 第52-54页 |
| ·Drought tolerance is higher in the dpe2-5 mutant than wild type Columbia-0 | 第54-56页 |
| ·Creating transgenic plants and analysis | 第56-60页 |
| ·The localization of DPE2 in plants by confocal microscopy | 第60-62页 |
| ·DPE2 protein showed rapid aggregation after cold treatment | 第62-63页 |
| ·DPE2 enzymatic activity was reduced after cold treatment | 第63-64页 |
| ·Over-expression of DPE2 complements dpe2-5 size and freezing tolerance | 第64-66页 |
| ·Transcription level of cold stress-induced Genes in the dpe2-5 mutant | 第66-68页 |
| ·Summary and Discussion | 第68-72页 |
| Bibliography | 第72-77页 |
| Acknowledgements | 第77页 |
