| 摘要 | 第4-6页 |
| Abstract | 第6-7页 |
| Contents | 第8-11页 |
| 1 INTRODUCTION | 第11-19页 |
| 1.1 Aim | 第11页 |
| 1.2 Rationale | 第11-18页 |
| 1.3 The key issue of optimization | 第18-19页 |
| 2 MATERIALS AND METHODS | 第19-30页 |
| 2.1 Bone preparation | 第19-20页 |
| 2.2 Demineralization | 第20页 |
| 2.3 Decellularization | 第20页 |
| 2.4 nSiHA and HA production | 第20-21页 |
| 2.5 DBM/dDBM morphology characterization - Evidence of having osteogenic and osteoconductive components - Scanning electron microscopy (SEM) | 第21页 |
| 2.6 DBM/dDBM functional groups characterization - Evidence of having osteogenic and osteoconductive components - Fourier transform infrared (FT-IR) spectroscopy) | 第21页 |
| 2.7 DBM/dDBM cellular characterization - Evidence of having osteogenic and osteoconductive components - Immunohistological & histological staining | 第21-22页 |
| 2.8 Incorporation of DBM/dDBM and HA/nSiHA | 第22页 |
| 2.9 Scaffold fabrication and substrate distribution | 第22-23页 |
| 2.10 HA/nSiHA characterization | 第23页 |
| 2.11 Local pH | 第23-24页 |
| 2.12 Mass loss (lyophilization) and SEM (degradation) | 第24页 |
| 2.13 Sterilization | 第24-25页 |
| 2.14 Cell culture | 第25页 |
| 2.15 Cell seeding and culture in vitro | 第25-26页 |
| 2.16 Cytotoxicity - Cck8 assay | 第26页 |
| 2.17 Cytotoxicity - Scanning electron microcopy (SEM) | 第26-27页 |
| 2.18 Osteogenic induction | 第27页 |
| 2.19 Differentiation - Characterization of osteogenic differentiation using morphological features - Optical microscopy & immunohistological staining (DAPI) | 第27页 |
| 2.20 Differentiation - Alkaline phosphatase activity (ALP) | 第27-30页 |
| 3 EVALUATION PLAN | 第30-33页 |
| 3.1 Lyophilization and decellularization | 第30页 |
| 3.2 pH | 第30-31页 |
| 3.3 Cytotoxicity | 第31-32页 |
| 3.4 Differentiation | 第32-33页 |
| 4 RESULTS | 第33-77页 |
| 4.1 DBM/dDBM production and lyophilization | 第33-35页 |
| 4.2 nSiHA and HA production | 第35页 |
| 4.3 DBM/dDBM morphology characterization - Evidence of having osteogenic and osteoconductive components - Scanning electron microscopy (SEM) | 第35-41页 |
| 4.4 DBM/dDBM functional groups characterization - Evidence of having osteogenic and osteoconductive components - Fourier transform infrared (FT-IR) spectroscopy | 第41-45页 |
| 4.5 DBM/dDBM cellular characterization - Evidence of having osteogenic and osteoconductive components | 第45-55页 |
| 4.6 HA and nSiHA characterization – SEM and EDX | 第55-58页 |
| 4.7 Local pH | 第58-63页 |
| 4.8 Degradation - SEM | 第63-64页 |
| 4.9 Cytotoxicity | 第64-67页 |
| 4.10 Differentiation | 第67-77页 |
| 5 DISCUSSION | 第77-82页 |
| 6 CONCLUSION | 第82-83页 |
| Acknowledgements | 第83-84页 |
| REFERENCES | 第84-88页 |
