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不同非生物胁迫下节节麦种群遗传多样性及候选参考基因的筛选

中文摘要第6-8页
Abstract第8-9页
Abbreviations第13-18页
CHAPTER 1 Literature Review第18-27页
    1.1 Aegilops tauschii Coss第18页
    1.2 Phylogenetic relationships between Triticum and Aegilops tauschii第18-19页
    1.3 Aegilops tauschii and genetic diversity第19-21页
    1.4 Aegilops tauschii and abiotic stresses第21页
    1.5 Aegilops tauschii and salinity stress第21-23页
    1.6 Aegilops tauschii, drought stress and chlorophyll fluorescence第23-24页
    1.7 Aegilops tuaschii and candidate references genes第24-26页
    1.8 Objectives of study第26-27页
CHAPTER 2 Genetic diversity Aegilops tauschii Coss populations from China by usingSSR markers and morphological traits第27-36页
    2.1 Introduction第27页
    2.2 Material and methods第27-30页
        2.2.1 Statistical analysis第28-30页
    2.3 Results第30-31页
    2.4 Discussions第31-36页
CHAPTER 3 Microsatellite-based genetic diversity and synergistic modulation of salinitytolerance genes in Aegilops tauschii Coss第36-49页
    3.1 Introduction第36-37页
    3.2 Material and Methods第37-39页
        3.2.1 Molecular Marker第37-38页
        3.2.2.RNA extraction第38页
        3.2.3 q PCR analysis第38页
        3.2.4 Data analysis第38-39页
    3.3 Results第39-45页
        3.3.1 Physiological traits第39-41页
        3.3.2 Molecular markers第41-44页
        3.3.3 HKT1;4 and NHX1 expression leaves and roots第44页
        3.3.4 Expression patterns of HKT1;4 in roots第44页
        3.3.5 Expression patterns of NHX1 in Leaves第44-45页
    3.4 Discussion第45-49页
        3.4.1 Perspective about salinity tolerance and genetic diversity第45-46页
        3.4.2 Role and expression pattern of salinity tolerance genes第46-47页
        3.4.3 Synergistic model of salinity tolerance第47-49页
CHAPTER 4 Effect of Drought Stress on Chlorophyll, Fluorescence, and Biomassportioning of Aegilops tauschii Coss第49-57页
    4.1 Introduction第49-50页
    4.2 Material and methods第50-51页
        4.2.1 Plant materials, growth conditions, and stress treatments第50页
        4.2.2 Chlorophyll fluorescence第50-51页
        4.2.3 Growth parameters第51页
        4.2.4 Statistical analysis第51页
    4.3 Results第51-54页
        4.3.1 Effect of different field capacity level on growth parameters第51-52页
        4.3.2 Effect of different field capacity level on photosynthetic activity第52-54页
    4.4 Discussion第54-57页
CHAPTER 5 Selection of suitable reference genes for gene expression studies第57-71页
    5.1 Introduction第57-58页
    5.2 Materials and Methods第58-61页
        5.2.1 Plant materials and treatments第58页
        5.2.2 RNA extraction c DNA synthesis第58页
        5.2.3 Selection of reference genes and primer design第58页
        5.2.4 q PCR analysis第58-59页
        5.2.5 Data analysis第59页
        5.2.6 Expression levels of NHXI and DRP1 and validation of reference gene第59-61页
    5.3 Results第61-68页
        5.3.1 Primer selection and q RT-PCR efficiency第61页
        5.3.2 Expression profile of reference genes第61页
        5.3.3 Genorm analysis第61-65页
        5.3.4 Normfinder analysis第65-66页
        5.3.5 Bestkeeper analysis第66-67页
        5.3.6 Refinder analysis第67-68页
        5.3.7 Validation of the most and least stable genes第68页
    5.4 Discussion第68-71页
CHAPTER 6 General Discussion第71-75页
CHAPTER 7 Conclusion第75-77页
References第77-92页
Acknowledgments第92-93页
Author Biography第93-96页

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