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多室电泳分离系统的构建及其在蛋白质和小分子分离中的应用

摘要第6-7页
Abstract第7-8页
Contents第9-10页
List of Figure and Table第10-11页
1. Introduction第11-16页
2. Materials and method第16-21页
    2.1 Materials and chemicals第16-17页
    2.2 Preparation of standard and sample solution第17页
    2.3 Electrophoresis separation system第17-20页
    2.4 Reversed phase liquid chromatography第20-21页
3. Results and Discussion第21-32页
    3.1 The construction of multi-compartment electrophoresis separation system第21-24页
        3.1.1 The separating system第21-23页
            3.1.1.1 The distance between the membranes第21-22页
            3.1.1.2 The choice of electrophoresis compartment material第22-23页
        3.1.2 The rest of system第23-24页
    3.2 The optimization of experimental conditions第24-29页
        3.2.1 Experiences with different voltage第24-25页
        3.2.2 Experiments with different electrophoretic time第25-27页
        3.2.3 Experiments with different types of membrane第27-28页
        3.2.4 Experiments with different buffer concentration第28-29页
    3.3 Method validation第29-30页
    3.4 Application in separation of protein and small molecule第30-32页
4. Conclusion第32-33页
References第33-39页
致谢第39-40页
附录A第40页

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