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Evaluation of Adjuvants Effects of Water Soluble Polysaccharide from the Stem of Physalis Alkekengi L.var.francheti (Mast) Makino in a Recombinant Protein Vaccine

ABSTRACT第4页
摘要第5-10页
CHAPTER 1 INTRODUCTION第10-30页
    1.1 Introduction of Physalis alkekengi L.var francheti (Mast) Makino第10-15页
        1.1.1 Background History of P. alkekengi第12-13页
        1.1.2 Plant Morphology and Classification of P. alkekengi第13-14页
        1.1.3 Health benefits of P. alkekengi第14-15页
    1.2 BACKGROUND ON ADJUVANT第15-23页
        1.2.1 Types of Adjuvants第16-18页
            1.2.1.1 Aluminum Salts第16-17页
            1.2.1.2 Emulsion-based Adjuvants第17页
            1.2.1.3 Saponin-based Adjuvants第17-18页
        1.2.2 Mechanism of Action of Adjuvants第18-20页
        1.2.3 The Essence of Adjuvants in a Typical Vaccine第20-22页
        1.2.4 Recent Advances in Adjuvants第22-23页
        1.2.5 The Characteristics of a Perfect Vaccine Adjuvant第23页
    1.3 BACKGROUND ON POLYSACCHARIDES第23-26页
        1.3.1 Immunological Polysaccharide第24-26页
            1.3.1.1 Glucans第24页
            1.3.1.2 α-glucans (Dextrans)第24-25页
            1.3.1.3 Fructans第25页
            1.3.1.4 Mannans第25-26页
    1.4 INTRODUCTION OF SYSTEMIC CANDIDIASIS第26-27页
    1.5 RESEARCH OBJECTIVES第27-28页
    1.6 JUSTIFICATION OF STUDY第28-30页
CHAPTER 2 MATERIALS AND METHOD第30-36页
    2.1 MATERIALS第30页
    2.2 PREPARATION OF RECOMBINANT PROTEIN (rP-HSP90C)第30-32页
        2.2.1 Purification of Recombinant Protein第31-32页
    2.3 ANIMAL EXPERIMENT第32页
        2.3.1 Immunization of Mice and Collection of Serum第32页
    2.4 ENZYME LINKED IMMUNOSORBENT ASSAYS (ELISA) TO DETERMINE IgG, IgG1 AND IgG2 ANTIBODIES第32-34页
    2.5 ELISPOT to detect IL concentration in serum of mice第34-35页
        2.5.1 ELISPOT to detect IL-10 concentration in serum of mice第34页
        2.5.2 ELISPOT to detect IL-12 concentration in serum of mice第34-35页
    2.6 STATISTICAL ANALYSIS第35-36页
CHAPTER 3 RESULTS第36-41页
    3.1 Antibody responses against rP-HSP90C in sera of mice immunized with rP-HSP90C and WSP第36-39页
        3.1.1 Detection of Titer of IgG In Serum of Mice Immunized with WSP and rP-HSP90C第36-37页
        3.1.2 Detection of Titer of IgG1 in Serum of Mice Immunized with WSP and rP-HSP90C第37-38页
        3.1.3 Detection of Titer of IgG2b in Serum of Mice Immunized with WSP and rP-HSP90C第38-39页
    3.2 Detection of IL Concentrations in Mice Serum第39-41页
        3.2.1 ELISPOT to detect IL-10 concentrations in mice serum第39-40页
        3.2.2 ELISPOT to detect IL-12 concentrations in mice serum第40-41页
CHAPTER 4 DISCUSSION第41-43页
CHAPTER 5 CONCLUSION第43-44页
REFERENCE第44-51页
DEDICATION第51-52页
ACKNOWLEDGEMENT第52页

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