| Abstract | 第9-11页 |
| Chapter 1: Bacillus species as versatile weapons for plant pathogens: a review | 第12-36页 |
| 1.1 Introduction | 第12-19页 |
| 1.2 Lipopeptides and antibiotics based weapons | 第19-20页 |
| 1.3 Plant growth promotion as indirect weapon | 第20-22页 |
| 1.4 Systemically induced disease resistance | 第22-26页 |
| 1.4.1 Trigging of Phenolic compounds | 第22-25页 |
| 1.4.2 Structural and genetic activation of host plants | 第25-26页 |
| 1.5 Activation of plant resistant activator | 第26-30页 |
| 1.5.1 Enzymatic weapons | 第27-30页 |
| 1.6 Colonization | 第30-32页 |
| 1.7 Remarks | 第32-36页 |
| Chapter 2: Isolation, Identification and screening of potential antagonistic bacteria against Botrytis cinerea | 第36-52页 |
| 2.1 Introduction and objectives | 第36页 |
| 2.2 Materials and methods | 第36-44页 |
| 2.2.1 Microbial isolation | 第36页 |
| 2.2.2 Selection of the biocontrol agent | 第36-37页 |
| 2.2.3 Identification of the selected antifungal bacterium | 第37-43页 |
| 2.2.4 Assays for testing biocontrol activities | 第43-44页 |
| 2.2.5 Ultrastructural studies | 第44页 |
| 2.2.6 Statistical analysis | 第44页 |
| 2.3 Results | 第44-51页 |
| 2.3.1 Isolation and selection of potential biocontrol agent | 第44页 |
| 2.3.2 Identification of the isolate | 第44-47页 |
| 2.3.3 Anti-Botrytis activity of strain JS-786 | 第47-50页 |
| 2.3.4 Effect of strain JS-786 on ultrastructure of B. cinerea | 第50-51页 |
| 2.4 Discussion | 第51-52页 |
| Chapter 3: Optimization of cell dry mass and antifungal using response surface methodology | 第52-65页 |
| 3.1 Introduction and objectives | 第52-53页 |
| 3.2 Materials and methods | 第53-55页 |
| 3.2.1 Culture medium and fermentation conditions | 第53页 |
| 3.2.2 Optimization of fermentation conditions | 第53页 |
| 3.2.3 Estimation of cell dry mass | 第53页 |
| 3.2.4 Estimation of antifungal activity | 第53-54页 |
| 3.2.5 Data analysis | 第54-55页 |
| 3.3 Results | 第55-63页 |
| 3.3.1 Optimization of culture conditions for cell dry mass and antifungal activity | 第55-63页 |
| 3.4 Discussion | 第63-64页 |
| 3.5 Conclusion | 第64-65页 |
| Chapter 4: Antimicrobial metabolites from Bacillus areus strain JS-786 and theireffects on morphology, physiology and ultrastructure of Botrytiscinerea | 第65-82页 |
| 4.1 Introduction and objectives | 第65页 |
| 4.2 Materials and Methods | 第65-70页 |
| 4.2.1 Biocontrol agent and phytopathogen | 第65-66页 |
| 4.2.2 Preparation bacterial seed culture | 第66页 |
| 4.2.3 Fermentation of biocontrol agent | 第66页 |
| 4.2.4 Extraction of metabolites | 第66页 |
| 4.2.5 Silica gel column chromatography | 第66-67页 |
| 4.2.6 Effect on spore germination | 第67页 |
| 4.2.7 Effect on mycelial growth | 第67页 |
| 4.2.8 GC-MS profiling | 第67-68页 |
| 4.2.9 Effect on ultrastructure of B.cinerea | 第68页 |
| 4.2.10 Determination of electrical conductivity and ergosterol contents | 第68-69页 |
| 4.2.11 Determination of fatty acids contents in B. cinerea | 第69页 |
| 4.2.12 Determination of SOD, POD and PAL activities | 第69-70页 |
| 4.2.13 Statistical analysis | 第70页 |
| 4.3 Results | 第70-79页 |
| 4.3.1 Effect on Spore germination and mycelial growth | 第70-71页 |
| 4.3.2 Effect on cell wall, cell membrane integrity and other defense reactions | 第71-73页 |
| 4.3.3 Scanning and transmission electron microscopy | 第73-77页 |
| 4.3.4 GC/MS analysis of antimicrobial metabolites | 第77-79页 |
| 4.4 Discussion | 第79-82页 |
| Chapter 5: Stimulation plant defence system against tomato gray mold and plantgrowth by Bacillus aerius strain JS-786 | 第82-105页 |
| 5.1 Introduction and objectives | 第82-83页 |
| 5.2 Materials and Methods | 第83-86页 |
| 5.2.1 Biocontrol agent and pathogen | 第83-84页 |
| 5.2.2 Formulation strain JS-786 | 第84页 |
| 5.2.3 Planting material and growing conditions | 第84页 |
| 5.2.4 Application of Strain JS-786 | 第84页 |
| 5.2.5 Recording of plant growth parameters | 第84-85页 |
| 5.2.6 Pathogen inoculation | 第85页 |
| 5.2.7 Assays for defense reactions | 第85-86页 |
| 5.2.8 Statistical Analysis | 第86页 |
| 5.3 Results | 第86-98页 |
| 5.3.1 Effect of biocontrol agent on disease severity | 第86-87页 |
| 5.3.2 Effect of chitinase activity in tomato leaves | 第87-88页 |
| 5.3.3 Effect of biocontrol agent on root and shoot growth of tomato | 第88-90页 |
| 5.3.4 Effect of biocontrol agent on gas exchange of tomato leaves | 第90-91页 |
| 5.3.5 Accumulation of reactive oxygen species in tomato leaves | 第91-94页 |
| 5.3.6 Activities of enzymatic and non-enzymatic antioxidants in tomato leaves | 第94-98页 |
| 5.4 Discussion | 第98-105页 |
| Chapter 6: Conclusion and Novelty of research | 第105-109页 |
| 6.1 Conclusion | 第105-108页 |
| 6.2 Novelty of research | 第108-109页 |
| References | 第109-134页 |
| List of publications | 第134-135页 |
| Acknowledgements | 第135-136页 |
| Statistics of tables and figures in the thesis | 第136-137页 |
