| 摘要 | 第11-15页 |
| Abstract | 第15-19页 |
| Chapter 1 Introduction | 第20-41页 |
| 1.1 Heavy metal pollution and remediation | 第20-21页 |
| 1.1.1 Sources and harmful effects of heavy metals pollution | 第20-21页 |
| 1.1.2 Remediation of heavy metals pollution | 第21页 |
| 1.2 Phytoremediation | 第21-22页 |
| 1.2.1 Define of phytoremediation | 第21-22页 |
| 1.2.2 Plants selection for phytoremediation | 第22页 |
| 1.3 Application and research of Salix in phytoremediation | 第22-38页 |
| 1.3.1 Application of Salix in phytoremediation | 第22-29页 |
| 1.3.2 Factors in accumulation and transportation of heavy metals by Salix | 第29-31页 |
| 1.3.3 Heavy metals accumulation in plant tissues | 第31-38页 |
| 1.4 Research objectives | 第38-41页 |
| Chapter 2 Effect of heavy metals combined stress on growth and metalsaccumulation of three Salix species with different cutting position | 第41-59页 |
| 2.1 Introduction | 第41-43页 |
| 2.2 Materials and methods | 第43-45页 |
| 2.2.1 Plant materials and treatments | 第43-44页 |
| 2.2.2 Growth parameters and Tolerance index (Ti) measurement | 第44-45页 |
| 2.2.3 Heavy metal and Translocation factor (TF) analyses | 第45页 |
| 2.2.4 Statistical analysis | 第45页 |
| 2.3 Results and discussion | 第45-57页 |
| 2.3.1 Growth parameters, biomass production, and Tolerance index ofSalix species | 第45-50页 |
| 2.3.2 HM accumulation and above-ground TF-aerial of the Salix species | 第50-52页 |
| 2.3.3 Growth parameters of different cutting position | 第52-56页 |
| 2.3.4 HM content in different cutting positions | 第56-57页 |
| 2.4 Conclusion | 第57-59页 |
| Chapter 3 Phytoextraction of initial cutting of S. matsudana for Cd and Cu | 第59-77页 |
| 3.1 Introduction | 第59-61页 |
| 3.2 Materials and methods | 第61-64页 |
| 3.2.1 Plant materials and treatments | 第61-63页 |
| 3.2.2 Growth parameters and Relative growth rate (RGR) determination | 第63页 |
| 3.2.3 Biomass and Tolerance index (Ti) measurement | 第63页 |
| 3.2.4 Heavy metal and Translocation factor (TF) analyses | 第63-64页 |
| 3.2.5 Phytoextraction capacity (PC) analysis | 第64页 |
| 3.2.6 Statistical analysis | 第64页 |
| 3.3 Results and Discussion | 第64-76页 |
| 3.3.1 Effect of cutting diameter on willow growth and its HMabsorptivity | 第64-68页 |
| 3.3.2 Root system function in vegetation filters | 第68-72页 |
| 3.3.3 Phytoextraction intensity of initial cuttings without rhizofiltration | 第72-74页 |
| 3.3.4 Interactions between Cd and Cu in phytoremediation | 第74-76页 |
| 3.4 Conclusion | 第76-77页 |
| Chapter 4 Effects of single and compound Cd and Zn treatment on the growth andphysiology of S. matsudana | 第77-90页 |
| 4.1 Introduction | 第77-78页 |
| 4.2 Materials and methods | 第78-82页 |
| 4.2.1 Plant materials and treatments | 第78-79页 |
| 4.2.2 Biomass production and Relative growth rate (RGR) determination | 第79页 |
| 4.2.3 Heavy metal analyses | 第79-80页 |
| 4.2.4 Enzyme activity measurement | 第80-81页 |
| 4.2.5 Chlorophyll measurement | 第81页 |
| 4.2.6 Root activity | 第81-82页 |
| 4.2.7 Statistical analysis | 第82页 |
| 4.3 Result and Discussion | 第82-89页 |
| 4.3.1 RGR and biomass of S. matsudana with single and compoundstress of Cd and Zn | 第82-84页 |
| 4.3.2 Chlorophyll content and Root activity of S. matsudana with singleand compound stress of Cd and Zn | 第84-85页 |
| 4.3.3 Antioxidant enzymes activity of S. matsudana with single andcompound stress of Cd and Zn | 第85-87页 |
| 4.3.4 Heavy metals concentration in different tissues of S. matsudanawith single and compound stress of Cd and Zn | 第87-89页 |
| 4.4 Conclusion | 第89-90页 |
| Chapter 5 Effects of single heavy metal stress on the growth and physiology of S.matsudana under unity concentration of lead, cadmium, zinc and copper | 第90-105页 |
| 5.1 Introduction | 第90-92页 |
| 5.2 Materials and methods | 第92-96页 |
| 5.2.1 Plant materials and treatments | 第92-93页 |
| 5.2.2 Biomass production and Relative growth rate (RGR) determination | 第93页 |
| 5.2.3 Heavy metal analyses | 第93页 |
| 5.2.4 Enzyme activity measurement | 第93页 |
| 5.2.5 Chlorophyll measurement and root activity | 第93-94页 |
| 5.2.6 Estimation of lipid peroxidation | 第94页 |
| 5.2.7 Soluble protein measurement | 第94-95页 |
| 5.2.8 Free proline measurement | 第95页 |
| 5.2.9 Statistical analysis | 第95-96页 |
| 5.3 Result and Discussion | 第96-104页 |
| 5.3.1 RGR and biomass of S. matsudana with different heavy metaltreatments | 第96-97页 |
| 5.3.2 Total chlorophyll content of S. matsudana with different heavymetal treatments | 第97-99页 |
| 5.3.3 Free proline content and root activity of S. matsudana withdifferent heavy metal treatments | 第99-100页 |
| 5.3.4 Antioxidant enzymes activity of S. matsudana with different heavymetal treatments | 第100-101页 |
| 5.3.5 MDA and soluble protein content of S. matsudana with differentheavy metal treatments | 第101-103页 |
| 5.3.6 Heavy metals concentration in different tissues of S. matsudanawith different heavy metal treatments | 第103-104页 |
| 5.4 Conclusion | 第104-105页 |
| Chapter 6 Effect of P fertilization on the growth and heavy metal accumulation of S.matsudana | 第105-125页 |
| 6.1 Introduction | 第105-107页 |
| 6.2 Materials and methods | 第107-110页 |
| 6.2.1 Plant materials and treatments | 第107-108页 |
| 6.2.2 Growth parameters and Relative growth rate (RGR) determination | 第108-109页 |
| 6.2.3 Biomass and Tolerance index (Ti) measurement | 第109页 |
| 6.2.4 Heavy metal and Translocation factor (TF) analyses | 第109-110页 |
| 6.2.5 Statistical analysis | 第110页 |
| 6.3 Results and discussion | 第110-124页 |
| 6.3.1 Growth and biomass of S. matsudana | 第110-115页 |
| 6.3.2 Weekly data of Salix matsudana with P and Cd addition treatment | 第115-118页 |
| 6.3.3 Tolerance index (Ti) and Translocation factor (TF) of Salixmatsudana | 第118-121页 |
| 6.3.4 Concentration and content of Cd in Salix matsudana with P and Cdaddition treatment | 第121-124页 |
| 6.4 Conclusion | 第124-125页 |
| Chapter 7 Effect of hydroponic pre-treatment on growth and physiology of Smatsudana after transplant to contaminate soil | 第125-146页 |
| 7.1 Introduction | 第125-127页 |
| 7.2 Materials and methods | 第127-129页 |
| 7.2.1 Plant materials and treatments | 第127-128页 |
| 7.2.2 Biomass production and Relative growth rate (RGR) determination | 第128页 |
| 7.2.3 Physiological index measurement | 第128页 |
| 7.2.4 Statistical analysis | 第128-129页 |
| 7.3 Result and Discussion | 第129-144页 |
| 7.3.1 RGR of S. matsudana in different water culture | 第129-132页 |
| 7.3.2 Moisture content of S. matsudana in different treatments andculture ways | 第132-134页 |
| 7.3.3 Biomass production of S. matsudana before and after transplant tocontaminate soil | 第134-140页 |
| 7.3.4 Total chlorophyll content in leaves of S. matsudana in differenttreatments and culture ways | 第140-141页 |
| 7.3.5 Antioxidant enzymes activity of S. matsudana in differenttreatments and culture ways | 第141-143页 |
| 7.3.6 Root activity of S. matsudana in different treatments and cultureways | 第143-144页 |
| 7.4 Conclusion | 第144-146页 |
| Chapter 8 Summary | 第146-151页 |
| 8.1 Conclusions | 第146-150页 |
| 8.2 Prospects | 第150-151页 |
| References | 第151-188页 |
| List of publications (September 2013-December 2016) | 第188-189页 |
| Acknowledgments | 第189-190页 |
