| List of abbreviations and acronyms | 第10-13页 |
| 中文摘要 | 第13-14页 |
| abstract | 第14页 |
| CHAPTER One Introduction | 第15-28页 |
| 1.1 Nuclear factor Y (NF-Y) transcription factors | 第16-17页 |
| 1.2 Classification of rice NF-Y genes | 第17-20页 |
| 1.3 Literature review | 第20-23页 |
| 1.4 bZIP proteins | 第23-28页 |
| CHAPTER Two Functional characterization of NF-YB1,NF-YC12 and bHLH144 in rice(Oryza sativa L.) | 第28-59页 |
| 2.1 Materials and methods | 第28-34页 |
| 2.1.1 Plant growth conditions and phenotypical characterizations | 第28页 |
| 2.1.2 RNA isolation, qRT-PCR and mRNA in-situ hybridization | 第28-29页 |
| 2.1.3 Vector construction and plant transformation | 第29页 |
| 2.1.4 Bacterial-two-hybrid assay | 第29-30页 |
| 2.1.5 Purification of tag-fused proteins and in vitro pull-down assay | 第30页 |
| 2.1.6 Yeast-three-hybrid assay | 第30-31页 |
| 2.1.7 BIFC assay | 第31页 |
| 2.1.8 Yeast-one-hybrid assay | 第31-32页 |
| 2.1.9 Luciferase transient transcriptional activity assay | 第32页 |
| 2.1.10 Electrophoresis Mobility Shift Assay | 第32-33页 |
| 2.1.11 Chromatin immuno-precipitation (ChIP) and ChIP-PCR | 第33页 |
| 2.1.12 Cell-free degradation Assay | 第33-34页 |
| 2.2 Results | 第34-53页 |
| 2.2.1 NF-YB 1 is a seed-specific gene and its protein locates in nuclear and cytoplasm | 第34页 |
| 2.2.2 Knock-down of NF-YB1 reduced grain size and altered grain quality | 第34-39页 |
| 2.2.3 NF-YB1,NF-YC12 and bHLH144 forms a hereotrimer complex in a sequential order | 第39-42页 |
| 2.2.4 cmf-ycl2 and crbhlhl44 exhibited similar phenotype to crnf-yb1 in seed development | 第42-44页 |
| 2.2.5 NF-YB1 and NF-YC12co-regulate transcription of genes involves in starch biosynthess | 第44-47页 |
| 2.2.6 NF-YB1 binds to the G-box of Wx promoter to activate its transcription | 第47-50页 |
| 2.2.7 NF-YC12 and bHLH144 enhances NF-YB1 stability | 第50-53页 |
| 2.3 Discussion | 第53-58页 |
| 2.3.1 Comprehensive effects of NF-YB 1 on seed development | 第53-54页 |
| 2.3.2 NF-YB directly activates Wxvia binding to the G-box in promoter | 第54-56页 |
| 2.3.3 NF-YB1-YC12-bHLH144 works as a complex | 第56-58页 |
| 2.4 Conclusion | 第58-59页 |
| CHAPTER Three The regulatory mechanism of OsRISBZ1, a basic leucine zippertranscription factor involved in rice seed development | 第59-77页 |
| 3.1 Materials and methods | 第59-62页 |
| 3.1.1 Plant growth conditions and phenotypical characterizations | 第59页 |
| 3.1.2 RNA isolation andqRT-PCR | 第59-60页 |
| 3.1.3 Vector construction and plant transformation | 第60页 |
| 3.1.4 Purification of tag-fused proteins and in vitro pull-down assay | 第60-61页 |
| 3.1.5 In vitro Kinase Assay | 第61页 |
| 3.1.6 Luciferase transient transcriptional activity assay | 第61页 |
| 3.1.7 Electrophoresis Mobility Shift Assay | 第61-62页 |
| 3.2 Results | 第62-72页 |
| 3.2.1 Expression patterns of RISBZ1 in different tissue and phenotypical characterization of risbzl and WT | 第62-68页 |
| 3.2.2 RISBZ1 interact physically with SAPK10 | 第68-70页 |
| 3.2.3 RISBZlbinds to the G-box of Wx promoter to activate its transcription | 第70-72页 |
| 3.3 Discussion | 第72-75页 |
| 3.4 Perspective and future research | 第75-77页 |
| References | 第77-85页 |
| Supporting information | 第85-95页 |
| Acknowledgement | 第95-96页 |
| List of publication during postdoctoral | 第96-97页 |
| Permanent home address | 第97页 |
