| COMPLETION CERTIFICATE FOR RESEARCH WORK | 第4页 |
| Supervisor | 第4-5页 |
| ABSTRACT | 第5-6页 |
| 摘要 | 第7-9页 |
| Table of Contents | 第9-12页 |
| CHAPTER 1:LITERATURE REVIEW | 第12-22页 |
| 1.0 Introduction | 第12页 |
| 1.1 Prostate Cancer | 第12-13页 |
| 1.2 Apoptosis | 第13-15页 |
| 1.3 Cell Cycle Progression | 第15-17页 |
| 1.4 Reactive Oxygen Species(ROS) | 第17-18页 |
| 1.5 Mitochondrial Membrane Potential | 第18-20页 |
| 1.6 Western blotting | 第20页 |
| 1.7 Hypothesis of the Research Work | 第20-21页 |
| 1.8 Objectives of present study | 第21-22页 |
| CHAPTER 2:NATURAL COMPOUNDS CHEMOPREVENTIVE AGENTS | 第22-30页 |
| 2.0 Medicinal Plants | 第22-23页 |
| 2.1 Sesquiterpene Lactones Exerts Anti-proliferative Activity on Cancer Cells | 第23-24页 |
| 2.2 Flavonoids Exerts Anti-proliferative Activity on Cancer Cells | 第24页 |
| 2.3 Natural Compounds as Anti-Cancer Drugs | 第24-27页 |
| 2.3.1 Isoalantolactone | 第24-25页 |
| 2.3.2 Altholactone | 第25-26页 |
| 2.3.3 Pinocembrin | 第26-27页 |
| 2.4 Molecular Targets for Natural Compounds Chemopreventive Agents | 第27-30页 |
| CHAPTER 3:MATERIAL AND METHODS | 第30-35页 |
| 3.0 Natural Compounds Library and Screening | 第30页 |
| 3.1 Chemicals and Reagents | 第30-31页 |
| 3.2 Cell Culture | 第31页 |
| 3.3 Preparation of cell lysates for proteins | 第31页 |
| 3.4 Mechanisms of Study | 第31-35页 |
| 3.4.1 Cell Proliferation Assay(MTT) | 第31-32页 |
| 3.4.2 Morphological Observation | 第32页 |
| 3.4.3 Flow Cytometric Analysis of Cell Cycle | 第32页 |
| 3.4.4 Flow Cytometric Determination of Apoptosis | 第32-33页 |
| 3.4.5 Flow Cytometric Determination of Reactive Oxygen Species | 第33页 |
| 3.4.6 Flow Cytometric Determination of Mitochondrial Membrane Potential | 第33页 |
| 3.4.7 Western Blotting | 第33-34页 |
| 3.4.8 Statistical Analysis of Data | 第34-35页 |
| CHAPTER 4:RESULTS AND DISCUSSION | 第35-64页 |
| 4.1 Anti-proliferative Eff-cts of Isoalantolactone on Prostate Cancer Cells | 第35-36页 |
| 4.2 Isoalanto]actone Induced Morphological Changes and Cell Death in Prostate Cancer PC-3 Cells | 第36-37页 |
| 4.3 Isoalantolactone Induced G2/M Cell Cycle Arrest on PC3 Cells | 第37-39页 |
| 4.4 Isoalantolactone Induced Apoptotic Cell Death on PC-3 cells | 第39-41页 |
| 4.5 Isoalantolactone Increased Generation of ROS on PC-3 cells | 第41-43页 |
| 4.6 Isoalantolactone Decreased Mitochondrial Membrane Potential on PC-3 cells | 第43-45页 |
| 4.7 Isoalantolactone Regulated Apoptosis-Related Proteins on PC-3 cells | 第45-46页 |
| 4.8 Anti-proliferative Effects of Altholactone on Prostate Cancer DU145 Cells | 第46-48页 |
| 4.9 Altholactone Induced Morphological Changes and Cell Death on DU145 cells | 第48-50页 |
| 4.10 Altholactone Induced G2/M Cell Cycle Arrest on DU145 Cells | 第50-51页 |
| 4.11 Altholactone Induced Apoptotic Cell Death on DU145 cells | 第51-54页 |
| 4.12 Altholactone Regulated Apoptosis-Related Proteins on DU145 cells | 第54-56页 |
| 4.13 Pinocembrin Inhibited Growth of Prostate Cancer LNCaP Cells | 第56-57页 |
| 4.14 Pinocembrin Induced Morphological Changes and Cell Death on LNCaP cells | 第57-58页 |
| 4.15 Pinocembrin Induced S and G2/M Cell Cycle Arrest on LNCaP Cells | 第58-59页 |
| 4.16 Pinocembrin Induced Apoptotic Cell Death on Human Prostate Cancer LNCaP Cells | 第59-61页 |
| 4.17 Pinocembrin Decreased Mitochondrial Membrane Potential on Human Prostate Cancer LNCaP Cells | 第61-64页 |
| CHAPTER 5:CONCLUSION AND NOVEL FINDINGS | 第64-69页 |
| 5.0 Conclusion | 第64页 |
| 5.1 Novel Findings and Significance | 第64-66页 |
| 5.1.1 Isoalantolactone(IAL) | 第64-65页 |
| 5.1.2 Altholactone(AL) | 第65页 |
| 5.1.3 Pinocembrin(PCB) | 第65-66页 |
| 5.2 List of Publications | 第66-67页 |
| 5.3 List of Abbreviations | 第67-69页 |
| REFERENCES | 第69-81页 |
| Acknowledgements | 第81页 |
