| 摘要 | 第1-7页 |
| Abstract | 第7-10页 |
| Part Ⅰ Introduction | 第10-26页 |
| 1.Resonance Rayleigh scattering | 第10页 |
| 2.Gemini surfactant | 第10-12页 |
| 3.Introduction of heparin | 第12页 |
| 4.Introduction of chondroitin sulfate | 第12-13页 |
| 5.Introduction of mercury ions | 第13-14页 |
| 6.Main purpose of this thesis | 第14-15页 |
| References | 第15-26页 |
| Part Ⅱ Main research works | 第26-66页 |
| 1.The determination of critical micelle concentration of conventional surfactants and geminisurfactant by the resonance scattering spectral methods | 第26-36页 |
| ·Experimental parts | 第27页 |
| ·Materials | 第27页 |
| ·Instrumentation | 第27页 |
| ·General procedure | 第27页 |
| ·Results and discussion | 第27-33页 |
| ·SOS and FDS methods for the determination of critical micelle concentration of conventional surfactants | 第27-29页 |
| ·SOS and FDS methods for the determination of critical premicelle concentration of conventional surfactants | 第29-31页 |
| ·RRS, SOS and FDS methods for the determination of critical premicelle concentration of gemini surfactant | 第31-32页 |
| ·Discussion | 第32-33页 |
| ·Conclusion | 第33页 |
| Refercences | 第33-36页 |
| 2.Gemini surfactant applied to the heparin assay at the nanogram level by resonance Rayleighscattering method | 第36-48页 |
| ·Experimental parts | 第36-37页 |
| ·Apparatus | 第36页 |
| ·Reagents | 第36-37页 |
| ·General procedure | 第37页 |
| ·Results and discussion | 第37-45页 |
| ·Spectral characteristics | 第37-38页 |
| ·Optimum conditions for the reaction | 第38-41页 |
| ·Calibration curve and detection limit | 第41-42页 |
| ·Analytical applications | 第42-43页 |
| ·Reaction mechanism of the system | 第43-45页 |
| ·Conclusion | 第45-46页 |
| References | 第46-48页 |
| 3.Study on the interation of chondroitin sulfate (A) with cationic gemini surfactant by resonancescattering spectral methods | 第48-56页 |
| ·Experimental parts | 第48-49页 |
| ·Apparatus | 第48页 |
| ·Reagents | 第48-49页 |
| ·Experimental procedures | 第49页 |
| ·Results and discussion | 第49-54页 |
| ·Spectral characteristics | 第49-50页 |
| ·Optimum conditions for the reaction | 第50-53页 |
| ·Calibration curve and detection limit | 第53页 |
| ·Analytical applications | 第53-54页 |
| ·Reaction mechanism of the system | 第54页 |
| ·Conclusion | 第54-55页 |
| References | 第55-56页 |
| 4.Detection of mercury ions (Ⅱ) based on non-cross-linking aggregation of double-strandedDNA-capped gold nanoparticles by resonance Rayleigh scattering | 第56-66页 |
| ·Experimental parts | 第56-58页 |
| ·Reagents and chemicals | 第56页 |
| ·Instrumentation | 第56-57页 |
| ·Preparation and characterization of gold nanoparticles | 第57页 |
| ·Modification of the gold nanoparticles with the oligonucleotide probes to preparation of dsDNA-gold nanoparticles | 第57-58页 |
| ·Procedure of Hg~(2+) determination | 第58页 |
| ·Results and discussion | 第58-64页 |
| ·RRS spectra | 第58-59页 |
| ·The results of TEM image and the UV-Vis spectra | 第59-60页 |
| ·Optimum conditions for the reaction | 第60-61页 |
| ·Sensitivities of the method | 第61-62页 |
| ·Calibration curves and detection limit | 第62-63页 |
| ·Mechanism of the reaction | 第63-64页 |
| ·Conclusion | 第64页 |
| References | 第64-66页 |
| 致谢 | 第66-68页 |
| Appendix:paper published during the graduate | 第68页 |
