| Abstract | 第5-7页 |
| Chapter 1 Introduction and Literature Review | 第12-33页 |
| 1.1 Introduction to Polygonaceae family | 第12页 |
| 1.2 Introduction to Polygonum bistorta L. | 第12-14页 |
| 1.3 Constituents of Polygonum bistorta L. reported in literature | 第14-20页 |
| 1.4 Introduction to chromatography | 第20-22页 |
| 1.4.1 High performance liquid chromatography | 第20页 |
| 1.4.2 Preparative HPLC | 第20-21页 |
| 1.4.3 Column chromatography | 第21页 |
| 1.4.4 Thin layer chromatography | 第21页 |
| 1.4.5 Gas chromatography | 第21-22页 |
| 1.5 Identification and characterization techniques | 第22-24页 |
| 1.5.1 Fingerprinting | 第22页 |
| 1.5.2 Mass spectroscopy | 第22-24页 |
| 1.5.2.1 High performance liquid chromatography-mass spectrometry (HPLC-MS) | 第23页 |
| 1.5.2.2 Gas chromatography mass spectrometry (GC-MS) | 第23-24页 |
| 1.6 Previous multiple characterization techniques employed on PB | 第24-25页 |
| 1.7 Bioactive properties and bioactive constituents of herbal plants | 第25-31页 |
| 1.7.1 Cancer and its causes | 第25-27页 |
| 1.7.1.1 Plant constituents related to anticancer activity | 第26页 |
| 1.7.1.2 Phenolic compounds and anticancer activity | 第26-27页 |
| 1.7.2 Essential oils and their bioactive properties | 第27-31页 |
| 1.7.2.1 Essential oils and food preservation | 第28页 |
| 1.7.2.2 Natural preservatives: a better choice | 第28-29页 |
| 1.7.2.3 Antibacterial activity assay | 第29页 |
| 1.7.2.4 Extraction techniques for essential oils | 第29-30页 |
| 1.7.2.5 Identification of essential oils | 第30-31页 |
| 1.7.3 Other reported bioactive properties of Polygonum bistorta L | 第31页 |
| 1.8 Aims and purpose | 第31-33页 |
| Chapter 2 Effect of Mobile Phase Composition and pH on HPLC Separation ofRhizome of Polygonum bistorta L. | 第33-47页 |
| 2.1 Introduction | 第33页 |
| 2.2 Materials and method | 第33-35页 |
| 2.2.1 Collection of plant material | 第33-34页 |
| 2.2.2 Instrument and reagents | 第34页 |
| 2.2.3 Preparation of standard solutions | 第34页 |
| 2.2.4 Extraction of PB sample | 第34页 |
| 2.2.5 Chromatographic conditions | 第34-35页 |
| 2.3 Results and discussion | 第35-46页 |
| 2.3.1 Optimization of extraction conditions | 第35页 |
| 2.3.2 Optimization of chromatographic conditions | 第35-41页 |
| 2.3.2.1 Effect of mobile phase composition and gradient selection | 第35-38页 |
| 2.3.2.2 Effect of mobile phase pH | 第38-39页 |
| 2.3.2.3 Detection wavelength | 第39-40页 |
| 2.3.2.4 Other conditions | 第40-41页 |
| 2.3.3 Fingerprint identification | 第41页 |
| 2.3.4 Repeatability test for optimized gradient | 第41-43页 |
| 2.3.5 Other conditions studied at optimized gradient | 第43-46页 |
| 2.3.5.1 Change of column brand | 第43页 |
| 2.3.5.2 Change of mobile phase methanol to acetonitrile | 第43-44页 |
| 2.3.5.3 Increasing the buffer concentration from 30mM to 40mM | 第44-45页 |
| 2.3.5.4 Changing buffer from KH_2PO_4 to NH_4HCO_3 | 第45-46页 |
| 2.4 Conclusion | 第46-47页 |
| Chapter 3 Preparative Separation and Identification of Chemical Constituentsfrom Methanol-Water Extract of Polygonum bistorta L. and Evaluation of itsAnticancer Activity | 第47-69页 |
| 3.1 Introduction | 第47-48页 |
| 3.2 Materials and methods | 第48-50页 |
| 3.2.1 Chemicals and materials | 第48页 |
| 3.2.2 Instruments | 第48页 |
| 3.2.3 Extraction | 第48页 |
| 3.2.4 Preparative chromatography conditions | 第48-49页 |
| 3.2.5 GC-MS conditions for obtained fractions | 第49页 |
| 3.2.6 HPLC-DAD-ESI-MS conditions | 第49-50页 |
| 3.2.7 Cell culture | 第50页 |
| 3.2.8 Cytotoxicity assay | 第50页 |
| 3.3 Results and discussion | 第50-68页 |
| 3.3.1 Optimization of preparative chromatographic conditions | 第50-51页 |
| 3.3.2 Identification of constituents in obtained fractions | 第51-61页 |
| 3.3.3 Other compounds identified from the fractions and raw extract | 第61-65页 |
| 3.3.4 Cytotoxicity | 第65-68页 |
| 3.4 Conclusion | 第68-69页 |
| Chapter 4 Difference in Composition and Antibacterial Activity of EssentialOils of Polygonum bistorta L. Obtained from Different Asian Origins | 第69-94页 |
| 4.1 Introduction | 第69-70页 |
| 4.2 Materials and method | 第70-72页 |
| 4.2.1 Plant material | 第70页 |
| 4.2.2 Instrument and reagents | 第70页 |
| 4.2.3 Extraction of essential oils | 第70-71页 |
| 4.2.4 Gas chromatography-mass spectrometry | 第71页 |
| 4.2.5 Identification of volatile constituents | 第71页 |
| 4.2.6 Antibacterial assay | 第71-72页 |
| 4.3 Results and discussion | 第72-93页 |
| 4.3.1 Composition of the essential oils | 第72页 |
| 4.3.2 Comparison and difference in composition of CH and PK essential oils | 第72-79页 |
| 4.3.3 Antibacterial constituents detected in essential oil | 第79-89页 |
| 4.3.3.1 Compounds detected with strong antibacterial activity | 第79-84页 |
| 4.3.3.2 Other antibacterial compounds | 第84-89页 |
| 4.3.4 Comparison of methanol-water extract of CH2 and PK2 samples | 第89-92页 |
| 4.3.5 Antibacterial activity of the essential oils | 第92页 |
| 4.3.6 Comparison of antibacterial activity of CH and PK essential oils | 第92-93页 |
| 4.4 Conclusion | 第93-94页 |
| Chapter 5 Conclusion | 第94-96页 |
| References | 第96-109页 |
| Acknowledgement | 第109-111页 |
| Publications and Presentations | 第111-112页 |
