| 摘要 | 第1-9页 |
| Abstract | 第9-11页 |
| 1 Introduction | 第11-23页 |
| ·The physicochemical properties and mechanism of Atrazine | 第11-12页 |
| ·The physicochemical properties of Atrazine | 第11-12页 |
| ·The mechanism of Atrazine | 第12页 |
| ·Present situation and application of atrazine pollution of atrazine in black soil area | 第12-14页 |
| ·Application of Atrazine | 第12-13页 |
| ·Pollution status in black soil region of Northeast China | 第13-14页 |
| ·The harm of atrazine pollution | 第14-18页 |
| ·Atrazine harm to ecological environment | 第15-17页 |
| ·Atrazine hazards to human health | 第17-18页 |
| ·Biodegradation of Atrazine | 第18-22页 |
| ·Phytoremediation of atrazine pollution | 第18-19页 |
| ·Bioremediation of atrazine contamination | 第19-21页 |
| ·Microbial Communities involved in atrazine degradation | 第21-22页 |
| ·Hypothesis | 第22页 |
| ·Aims and Objectives | 第22-23页 |
| 2 Materials and Methods | 第23-30页 |
| ·Soil Sampling | 第23页 |
| ·Chemicals and different carbon, nitrogen sources: | 第23页 |
| ·Material used for Bioremediation | 第23-24页 |
| ·Opted p H and Temperature Parameters | 第24页 |
| ·Experimental Design to Measure Impact of Soil p H and Temperature | 第24页 |
| ·Soil Treatment Preparation: | 第24-25页 |
| ·Preparation of Arthrobactor sp. Strain DNS10: | 第25页 |
| ·Experimental Design for Different Bioremediation Strategies: | 第25页 |
| ·Extraction and Determination of Atrazine from all Set Treatments: | 第25-26页 |
| ·Atrazine measurement and calculation: | 第26页 |
| ·Soil DNA extraction | 第26-27页 |
| ·Bacterial 16Sr DNA V3 PCR amplification of the variable region gene | 第27-28页 |
| ·Denaturing gradient gel electrophoresis (DGGE) method of operation | 第28-29页 |
| ·Statistical Analysis | 第29-30页 |
| 3 Results | 第30-42页 |
| ·Residual atrazine under different parameters | 第30-31页 |
| ·Atrazine removal percentage at different p H and temperatures | 第31-32页 |
| ·Temperature and p H significances on different bioremediation approaches | 第32-34页 |
| ·Extractable atrazine concentration in different selected sources | 第34-35页 |
| ·Atrazine degradation potential of different bioremediation strategies: | 第35-36页 |
| ·Percentage of atrazine bioremediation for each microcosm: | 第36-38页 |
| ·Correlation coefficient matrix for different bioremediation variables: | 第38-40页 |
| ·PCR-DGGE Anlaysis | 第40-42页 |
| 4 Discussion | 第42-50页 |
| ·Pesticide and herbicide contamination | 第42页 |
| ·Atrazine contamination | 第42页 |
| ·Atrazine bioremediation | 第42-43页 |
| ·Effective Bioremediation Techniques | 第43-44页 |
| ·Use of microbial strains in atrazine bioremediation | 第44-45页 |
| ·Different carbon and nitrogen sources utilized in atrazine bioremediation | 第45-46页 |
| ·Combined application of bioremediation treatments | 第46-47页 |
| ·Consequences of recent study | 第47-48页 |
| ·Impact of soil p H and temperature on atrazine bioremediation | 第48-50页 |
| Conclusion | 第50-51页 |
| Acknowledgements | 第51-52页 |
| References | 第52-59页 |
| Research Publication in Period of Ph. D Education | 第59页 |
