| ABSTRACT | 第1-13页 |
| 1 INTRODUCTION | 第13-28页 |
| ·Biological characteristics of sea buckthorn | 第13-14页 |
| ·Multi-purpose use of sea buckthorn | 第14-16页 |
| ·Chemical composition and chemotaxonomic study of sea buckthorn | 第16-19页 |
| ·Threats to sea buckthorn | 第19-20页 |
| ·Genetic diversity and conservation | 第20-22页 |
| ·PCR-based DNA fingerprinting | 第22-26页 |
| ·Objectives of this study | 第26-28页 |
| 2 MATERIALS AND METHODS | 第28-41页 |
| ·Species studied | 第28页 |
| ·Sample collection | 第28-29页 |
| ·Total DNA extraction and quantification | 第29-31页 |
| ·ISSR PCR amplification | 第31-32页 |
| ·Agarose gel electrophoresis | 第32页 |
| ·Analysis of chemical composition | 第32-33页 |
| ·Data analysis | 第33-41页 |
| 3 RESULTS | 第41-77页 |
| ·Genetic diversity of Hippophae rhamnoides ssp. sinensis | 第41-44页 |
| ·ISSR-PCR results | 第41-42页 |
| ·Genetic diversity of 11 populations | 第42页 |
| ·Nei's genetic distance | 第42页 |
| ·Cluster analysis | 第42页 |
| ·Analysis of genetic structure of 11 populations | 第42-44页 |
| ·Correlation between geographic and genetic distances matrix | 第44页 |
| ·Chemical components of 7 populations of Hippophae rhamnoides ssp. sinensis | 第44-56页 |
| ·GC-MS analysis | 第44-50页 |
| ·Variations in chemical components | 第50-52页 |
| ·Correlation between the chemical components and geographic variation | 第52页 |
| ·Correlation between the distances estimated using genetic and chemical markers | 第52-56页 |
| ·Genetic diversity of Hippophae rhamnoides ssp. yunnanensis | 第56-59页 |
| ·ISSR-PCR results | 第56-57页 |
| ·Genetic diversity of 7 populations | 第57页 |
| ·Nei's genetic distance | 第57-58页 |
| ·Cluster analysis | 第58页 |
| ·Analysis of genetic structure of 7 populations | 第58-59页 |
| ·Correlation between geographic and genetic distances matrix | 第59页 |
| ·Chemical components of 7 populations of Hippophae rhamnoides ssp. yunnanensis | 第59-67页 |
| ·GC-MS analysis | 第59-65页 |
| ·Variation of chemical components | 第65-67页 |
| ·Correlation between the chemical distances and geographic distances | 第67页 |
| ·Correlation between the genetic distances and chemical distances | 第67页 |
| ·Genetic diversity of Hippophae rhamnoides ssp. gyantsensis | 第67-69页 |
| ·Compariation of genetic diversity of Hippophae rhamnoides ssp. sinensis, ssp. yunnanensis and ssp. gyantsensis | 第69-71页 |
| ·Mean genetic diversity and Nei's genetic distances | 第69页 |
| ·Cluster analysis | 第69-71页 |
| ·Compariation of Chemical components of Hippophae rhamnoides ssp. sinensis and ssp. yunnanensis | 第71-77页 |
| ·Variation of chemical components | 第71-72页 |
| ·Cluster analysis of chemical distances | 第72-77页 |
| 4 DISCUSSION AND CONCULUSIONS | 第77-91页 |
| ·Genetic diversity and genetic structure of three subspecies of Hippophae rhamnoides | 第77-86页 |
| ·Chemical diversity and it's taxonomical implications | 第86-91页 |
| REFERENCES | 第91-104页 |
| APPENDIX 1 | 第104-127页 |
| ACKNOWLEDGEMENTS | 第127-128页 |
