| ACKNOWLEDGEMENT | 第1-7页 |
| Chinese Abstract | 第7-8页 |
| Abstract | 第8-16页 |
| CHAPTER ONE | 第16-31页 |
| ·INTRODUCTION | 第16页 |
| ·Oil Spill in Marine Environment | 第16-17页 |
| ·Properties of Oil in Marine Environment | 第17-18页 |
| ·Effects ofoil spill on Marine Environment | 第18-24页 |
| ·Effects ofoil spill on the Water | 第18-20页 |
| ·Effects ofoil spill on the Coastline | 第20-21页 |
| ·Effects ofoil spill on Marine Life and Wildlife | 第21-24页 |
| ·Bioremediation of spilled oil in marine environment | 第24-26页 |
| ·In-situ Bioremediation of oil spill in marine environment | 第25页 |
| ·Ex-situ Bioremediation ofoil spill in marine environment | 第25-26页 |
| ·Bacterial Community Dynamics during Bioremediation of Spilled oil-Contaminated Environments | 第26-28页 |
| ·Studies of Oil Spill Bioremediation in Mangrove | 第28-29页 |
| ·Objectives of the Present Study | 第29-31页 |
| CHAPTER TWO MATERIALS AND METHODS | 第31-43页 |
| ·MATERIALS | 第31-35页 |
| ·The Petroleum Oil test-Sample | 第31页 |
| ·Reagent,Material and Equipment | 第31-35页 |
| ·Kits | 第31页 |
| ·Strains,Plasmids,Vectors and Primers | 第31-32页 |
| ·Primers | 第32页 |
| ·Enzymes | 第32页 |
| ·Software | 第32页 |
| ·Filter Membrane | 第32页 |
| ·Reagents | 第32-33页 |
| ·Apparatus | 第33页 |
| ·Enrichment Media | 第33页 |
| ·MM2 medium per liter | 第33页 |
| ·Zobell 2216 E medium | 第33页 |
| ·R2A medium per liter | 第33-34页 |
| ·LB medium per liter | 第34页 |
| ·LB-Amp medium | 第34页 |
| ·Bacterial DNA extraction buffer | 第34页 |
| ·CTAB/NaCI solution | 第34页 |
| ·0.1MCaC1_2 | 第34页 |
| ·TAE(50x) | 第34页 |
| ·TE bufefr | 第34页 |
| ·Agarose gel eleetrophoresis samPle bufefr(6x) | 第34页 |
| ·TENP | 第34-35页 |
| ·Soil DNA extraction buffer | 第35页 |
| ·INT | 第35页 |
| ·X一gal storage solution | 第35页 |
| ·Isopropyl β-D-thiogalactoside(IPTG)Liquid | 第35页 |
| ·BASIC METHODS | 第35-41页 |
| ·Study area and sampling | 第35-37页 |
| ·Enrichment of mangrove surface sediment and test sample | 第37-39页 |
| ·Isolation and counting of cultivable bacteria clone from the enrichment mixture | 第39-40页 |
| ·Determination of Electron Transport System Activity(ETSA) of cultivatable bacteria in the enrichment mixture | 第40-41页 |
| ·MOLECULAR METHOD | 第41-43页 |
| ·Extraction of Total DNA | 第41页 |
| ·Putative Identification of Bacterial Species by Sequencing of DGGE Bands | 第41页 |
| ·Identification of the Isolates and the DGGE Bands | 第41页 |
| ·16S rDNA Amplification and PCR-DGGE Analysis | 第41-43页 |
| CHAPTER THREE | 第43-54页 |
| ·RESULT | 第43页 |
| ·Cultivable bacteria abundance in mangrove surface segment under spilled oil pollution | 第43-47页 |
| ·Cultivable bacteria numbers in the Non-transfer sample | 第43-44页 |
| ·Cultivable bacteria numbers in the transfer sample | 第44-47页 |
| ·Cultivable bacteria numbers in different enrichment stations | 第44-45页 |
| ·Cultivable bacteria numbers in the different enrichment media | 第45-46页 |
| ·Cultivable bacteria numbers in different oil enrichment samples | 第46-47页 |
| ·Bacterial metabolic activity in mangrove sediment under spilled oil pollution | 第47-48页 |
| ·ETSA in the non-transfer sample | 第47-48页 |
| ·ETSA in the transfer sample | 第48页 |
| ·ETSA in different enrichment station | 第48页 |
| ·ETSA in different enrichment media | 第48页 |
| ·ETSA in different oil enrichment sample | 第48页 |
| ·Bacteria Community Structure in mangrove segment under oil spill | 第48-53页 |
| ·Total DNA of sediment sample in station 15 | 第49-50页 |
| ·Total DNA of enrichment consortium in station 15 | 第50-53页 |
| ·Isolation and Identification of the oil degrading bacteria | 第53-54页 |
| CHAPTER FOUR | 第54-63页 |
| ·DISCUSSION OF RESULT | 第54页 |
| ·Cultivable bacteria abundance in mangrove surface segment under spilled oil pollution | 第54-58页 |
| ·Cultivable bacteria abundance in the non-transfer sample | 第54-55页 |
| ·Cultivable bacteria abundance in the transfer sample | 第55-58页 |
| ·Bacterial metabolic activity in mangrove sediment under spilled oil pollution | 第58-60页 |
| ·Bacterial metabolic activity in mangrove sediment under spilled oil pollution in the non-transfer sample | 第59页 |
| ·Bacterial metabolic activity in mangrove sediment under spilled oil pollution in the transfer sample | 第59-60页 |
| ·Analysis of Bacteria Community Structure in mangrove segment under oil spill using PCR-DGGE | 第60-62页 |
| ·Oil-pollution stress caused no changes in the structure of bacteria community structure | 第60-61页 |
| ·PCR-DGGE analysis of the strip | 第61-62页 |
| ·Isolation and Identification of the oil degrading bacteria culture | 第62-63页 |
| CHAPTER FIVE CONCLUSIONS AND RECOMMENDATIONS | 第63-66页 |
| ·Conclusions | 第63-64页 |
| ·Recommendations | 第64-65页 |
| ·Limitations | 第65-66页 |
| REFERENCES | 第66-70页 |
| APPENDIX | 第70-72页 |
| Abbreviations | 第70-71页 |
| Vector Map | 第71-72页 |
| DNA Molecular Weight Standards | 第72页 |
