| 摘要 | 第6-7页 |
| ABSTRACT | 第7页 |
| ABBREVIATION | 第16-17页 |
| CHAPTER 1 INTRODUCTION | 第17-29页 |
| 1.1 GENERAL INRODUCTION | 第17-20页 |
| 1.1.1 LITERATURE REVIEW | 第17页 |
| 1.1.2 BRIEF HISTORY OF PHYTOALEXIN | 第17-19页 |
| 1.1.3 PHYTOALEXINS | 第19页 |
| 1.1.4 PHYTOCHEMICALS | 第19-20页 |
| 1.2 BIOSYNTHESIS OF GLYCEOLLIN | 第20-26页 |
| 1.2.1 PHYTOCHEMICAL GLYCEOLLIN | 第23-24页 |
| 1.2.2 GLYCEOLLINS (I) THE ANTI ESTROGENIC COMPONENT | 第24页 |
| 1.2.3 HEALTH BENEFITS OF PHYTOCHEMICAL GLYCEOLLIN | 第24页 |
| 1.2.4 RESEARCH FINDINGS AND PROOF OF GLYCEOLLINS | 第24-26页 |
| 1.2.5 PRINCIPLES FOR ACTIVATING GLYCEOLLIN | 第26页 |
| 1.2.6 ELICITORS OF GLYCEOLLIN AND THEIR EFFECTS | 第26页 |
| 1.3 PROGRESS IN PHYTOCHEMICAL GLYCEOLLIN RESEARCH | 第26-27页 |
| 1.4 RESEARCH OBJECTIVE AND SUMMARY OF THE EXPERIM-ENTAL DESIGN | 第27-29页 |
| CHAPTER2 MORPHOLOGY AND MICROSCOPIC CHARACTERISTICS OFASPERGILLUS | 第29-31页 |
| 2.1 FUNGI (ASPERGILLUS) | 第29-31页 |
| 2.1.1 FUNGAL NUTRITION | 第29页 |
| 2.1.2 FUNGAL MORPHOLOGY AND MICROSCOPIC CHARACTERISTICS | 第29-30页 |
| 2.1.3 SUMMARY | 第30-31页 |
| CHAPTER 3 EXPERIMENTAL MATERIALS AND SELECTION OF CULTURINGMETHOD FOR GLYCEOLLINS EXTRACTION | 第31-38页 |
| 3.1 EXPERIMENTAL MATERIALS | 第31-32页 |
| 3.1.1 MATERIALS | 第31页 |
| 3.1.2 CHEMICALS | 第31-32页 |
| 3.1.3 POTATO DEXTROSE AGAR (PDA) | 第32页 |
| 3.1.4 STERILIZATION OF CULTURE MEDIA | 第32页 |
| 3.1.5 FUNGI GROWTH (ASPERGILLUS) | 第32页 |
| 3.2 S ELECTION OF CULTURING METHOD FOR GLYCEOLLIN EXTRACTION ...17 | 第32-38页 |
| 3.2.1 METHODS | 第33-34页 |
| 3.2.2 CULTURE AND EXTRACTION PROCEDURE FOR EXPERIMENT (1) | 第34页 |
| 3.2.3 EXTRACTION PROCEDURE | 第34-35页 |
| 3.2.4 CULTURE AND EXTRACTION PROCEDURE FOR EXPERIMENT (2) | 第35页 |
| 3.2.5 EXTRACTION PROCEDURE | 第35-37页 |
| 3.2.6 CONCLUSION | 第37-38页 |
| CHAPTER 4 PREPARATIVE ISOLATION AND PURIFICATION OFGLYCEOLLIN BY HIGH SPEED COUNTERCURRENTCHROMATOGRAPHY (HSCCC) | 第38-49页 |
| 4.1 INTRODUCTION | 第38页 |
| 4.2 EXPERIMENTAL MATERIALS AND METHODS | 第38-41页 |
| 4.2.1 APPARATUS | 第38-39页 |
| 4.2.2 CHEMICALS | 第39页 |
| 4.2.3 EXPERIMENTAL MATERIALS | 第39页 |
| 4.2.4 PROCESS FOR PREPARATION OF CRUDE SAMPLE | 第39-40页 |
| 4.2.5 MEASUREMENT OF PARTITION COEFFICIENT (K) | 第40-41页 |
| 4.3 PREPARATION OF TWO-PHASE SOLVENT SYSTEM FOR HSCCC | 第41-46页 |
| 4.3.1 ISOLATION AND PURIFICATION OF GLYCEOLLIN BY HSCCC | 第41页 |
| 4.3.2 ANALYSIS OF GLYCEOLLINS BY ANALYTICAL HPLC | 第41-42页 |
| 4.3.3 MASS SPECTROMETRY AND ULTRA PERFORMANCE LIQUIDCHROMATOGRAPHY (UPLC- MS) CONDITIONS4.3.4 RESULTS AND DISCUSSION | 第42页 |
| 4.3.4 RESULTS AND DISCUSSION | 第42-45页 |
| 4.3.5 HPLC ANALYSIS AND IDENTIFICATION OF HSCCC PEAK FRACTIONS | 第45-46页 |
| 4.4 CONFIRMATION OF STRUCTURE | 第46-48页 |
| 4.5 CONCLUSION: | 第48-49页 |
| CHAPTER 5 INDUCTION OF GLYCEOLLIN BY ASPERGILLUS SPECIES(BIOTIC ELICITOR) IN VARIETIES OF SOYBEAN SEEDS | 第49-64页 |
| 5.1 INTRODUCTION | 第49页 |
| 5.2 EXPERIMENTAL MATERIALS AND METHODS | 第49-50页 |
| 5.2.1 MATERIALS AND CHEMICALS | 第49页 |
| 5.2.2 FUNGAL CULTURES | 第49-50页 |
| 5.2.3 FUNGAL INOCULATION OF SOYBEAN COTYLEDONS | 第50页 |
| 5.3 SOYBEAN EXTRACTION AND SAMPLE PREPARATION | 第50-55页 |
| 5.3.1 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSISOF PHYT-OALEXINS | 第50页 |
| 5.3.2 RESULTS OF HPLC ANALYSIS OF ELICITED SOYBEAN SEEDS | 第50-53页 |
| 5.3.3 CALIBRATION CURVE | 第53-54页 |
| 5.3.4 EFFECT OF ELICITOR CONCENTRATION UPON GLYCEOLLININDUCTION | 第54-55页 |
| 5.3.5 THE SINGLE FACTOR EXPERIMENT | 第55页 |
| 5.4 EFFECTS OF SOYBEAN SEEDS SOAK TIME IN WATER | 第55-64页 |
| 5.4.1 THE TIME COURSE EXPERIMENT | 第56-58页 |
| 5.4.2 TEMPERATURE AS A SINGLE FACTOR EXPERIMENT | 第58-59页 |
| 5.4.3 IDENTIFICATION OF GLYCEOLLINS BY MASS SPECTROMETRY(ESI-MS) | 第59-61页 |
| 5.4.4 PREPARATIVE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY(PREP-HPLC) OF PHYT OALEXIN GLYCEOLLIN | 第61-62页 |
| 5.4.5 UPLC SPECTROMETRY ANALYSIS OF GLYCEOLLIN | 第62-64页 |
| CHAPTER 6 | 第64-66页 |
| 6.1 CONCLUSION AND RECOMMENDATION | 第64页 |
| 6.2 RECOMMENDATION | 第64-66页 |
| REFERENCES | 第66-72页 |
| APPENDIX | 第72-74页 |
| ACKNOWLEDGEMENT | 第74-75页 |
| RESUME | 第75页 |
