| 摘要 | 第1-7页 |
| Abstract | 第7-10页 |
| Part Ⅰ Introduction | 第10-32页 |
| 1. Introduction to fluorescence and fluorescence analysis method | 第10-18页 |
| ·Phenomenon of fluorescence | 第10-12页 |
| ·Molecular information from fluorescence | 第12-13页 |
| ·Several fluorescence technologies | 第13-15页 |
| ·Fluorescence probes | 第15-18页 |
| 2. Introduction to polyethyleneimine | 第18-19页 |
| 3. The objects in our study | 第19-22页 |
| 4. The significance of this study | 第22-23页 |
| References | 第23-32页 |
| Part Ⅱ Main research works | 第32-88页 |
| Chapter 1. Polyethyleneimine-capped silver nanoclusters as a fluorescence probe for sensitive detection of hydrogen peroxide and glucose | 第32-50页 |
| 1. Exerimental | 第33-35页 |
| ·Chemicals and reagents | 第33-34页 |
| ·Apparatus | 第34页 |
| ·Synthesis of polyethyleneimine-capped silver nanoclusters | 第34-35页 |
| ·Fluorescence measurements | 第35页 |
| 2. Results and discussion | 第35-46页 |
| ·Characterization of polyethyleneimine-capped silver nanoclusters | 第35-37页 |
| ·Fluorescence quenching of polyethyleneimine-capped silver nanoclusters by H_2O_2 | 第37-41页 |
| ·Detection of glucose based on fluorescent polyethyleneimine- capped silver nanoclusters | 第41-42页 |
| ·Selectivity for glucose detection | 第42-43页 |
| ·Analytical applications in real serum sample | 第43-45页 |
| ·Exploration of the quenching mechanism | 第45-46页 |
| 3. Conclusions | 第46-47页 |
| References | 第47-50页 |
| Chapter 2. Efficient fluorescence "turn-off" method for Cu~(2+) detection based onfluorophore-labeled DNA and polyethyleneimine | 第50-71页 |
| 1. Experamental | 第52-53页 |
| ·Apparatus | 第52页 |
| ·Reagents | 第52页 |
| ·Standard solutions | 第52-53页 |
| ·General procedure for copper (Ⅱ) analysis | 第53页 |
| 2. Results and discussion | 第53-64页 |
| ·Fluorescence response of the system and the mechanism | 第53-57页 |
| ·Optimum conditions for system | 第57-62页 |
| ·Sensitivity | 第62-64页 |
| ·Selectivity of the system | 第64页 |
| 3. Application | 第64-66页 |
| 4. Conclusion | 第66-67页 |
| References | 第67-71页 |
| Chapter 3. Efficient fluorescence "turn-on" method for sodium dodecyl sulfate basedon eosin Y and polyethyleneimine | 第71-88页 |
| 1. Experimental | 第73-75页 |
| ·Apparatus | 第73-74页 |
| ·Reagents | 第74页 |
| ·Standard solutions | 第74页 |
| ·General procedure for sodium dodecyl sulfate analysis | 第74-75页 |
| 2. Results and discussion | 第75-84页 |
| ·Spectral characteristics | 第75-76页 |
| ·Optimum conditions for system | 第76-80页 |
| ·Sensitivity | 第80-82页 |
| ·Selectivity | 第82页 |
| ·Detection of sodium dodecyl sulfate in real samples | 第82-84页 |
| 3. Conclusion | 第84-85页 |
| References | 第85-88页 |
| Part Ⅲ Conclusions | 第88-89页 |
| Appendix: Paper published during the graduate | 第89-90页 |
| 致谢 | 第90页 |
