| 摘要 | 第1-13页 |
| Abstract | 第13-14页 |
| Chapter 1. Introduction | 第14-24页 |
| ·Osteosarcoma | 第14页 |
| ·Metastasis | 第14-15页 |
| ·Cyclooxygenase(COX) | 第15-16页 |
| ·Location of COX-2 | 第16页 |
| ·Function of COX-2 | 第16-17页 |
| ·Regulation of COX-2 gene expression | 第17-18页 |
| ·Regulation of COX-2 | 第18页 |
| ·Gangliosides and ganglsioside GD1a | 第18-19页 |
| ·Caveolae and Cav-1 | 第19-21页 |
| ·Transcriptional regulation of COX-2 in FBJ cells | 第21-24页 |
| Chapter 2. Materials and methods | 第24-29页 |
| ·Cell lines and culture | 第24页 |
| ·Chemicals | 第24页 |
| ·Small interference RNA (siRNA) and siRNA transfection | 第24-25页 |
| ·SiRNA sequence constructed in a vector | 第24-25页 |
| ·The protocols of siRNA tranfection | 第25页 |
| ·RNA extraction and Reverse transcriptase PCR (RT-PCR) | 第25-29页 |
| ·The protocol of Total RNA Extraction | 第25-26页 |
| ·RT-PCR | 第26-28页 |
| ·Agarose eletrophoresis | 第28-29页 |
| Chapter 3. Results | 第29-32页 |
| ·GD1a may downregulate COX-2 expression | 第29-31页 |
| ·Down-regulation of Cav-1 expression induces COX-2 expression in FBJ-S1 cells | 第31-32页 |
| Chapter 4. Transcriptional regulation of COX-2 in FBJ cells | 第32-44页 |
| ·GSK-3β may not be involved in the regulation of COX-2 and Cav-1 expression | 第32-33页 |
| ·PI3K may be not involved in the regulation of COX-2 in FBJ-LL cells | 第33-34页 |
| ·TNFα and PDGF induce COX-2 expression in FBJ-LL cells | 第34-35页 |
| ·NFκB is involved in the regulation of COX-2 expression in FBJ-LL cells, but not in the COX-2 induced by TNFα | 第35-36页 |
| ·cAMP may stimulate COX-2 expression through EPAC1 pathway in FBJ-S1 cells. | 第36-38页 |
| ·Calcium signal may negatively regulate COX-2 expression in FBJ cells | 第38-40页 |
| ·PKC may be involved in the regulation of COX-2 in FBJ-LL cells | 第40-41页 |
| ·ERK1/2 may be involved in the regulation of COX-2 expression | 第41-44页 |
| Conclusions and discussions | 第44-45页 |
| Published paper | 第45-46页 |
| Acknowledgements | 第46-47页 |
| References | 第47-50页 |
