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超声波/微波复合萃取及酶法前处理对两种薰衣草中生物活性成分分离的研究

ACKNOWLEDGEMENTS第5-13页
Abstract第13-15页
摘要第16-24页
Chapter 1. General Introduction and Review Relevant Literature第24-43页
    1.1. Introduction第24页
    1.2. Ultrasound第24-31页
        1.2.1. Sonochemistry and cavitation phenomenon第25-26页
        1.2.2. Applications of ultrasound in food processes第26-31页
    1.3. Microwave irradiation第31-32页
        1.3.1. Basic principles第31页
        1.3.2. Microwave-assisted Extraction第31-32页
    1.4. Enzymatic hydrolysis-assisted第32页
    1.5. Aromatic and medicinal plant第32-33页
    1.6. Research justification and objectives第33-34页
        1.6.1. Main objective第33页
        1.6.2. Specific objectives第33-34页
    1.7. References第34-43页
Chapter 2. Effect of ultrasonic treatment on the total phenolic contents of crudeextracts from Lavandula pubescens and assess its bioactivity第43-67页
    2.1.Introduction第43-44页
    2.2.Material and methods第44-50页
        2.2.1. Plant sample第44页
        2.2.2. RBDPOo第44页
        2.2.3. Chemicals第44-45页
        2.2.4. CW-2000 Ultrasonic-microwave Cooperative Extractor/Reactor第45-46页
        2.2.5. Ultrasound-homogenizer第46页
        2.2.6. The initial characteristics of fresh RBDPOo第46页
        2.2.7. Extraction Procedures第46-47页
            2.2.7.1.Maceration extraction第46页
            2.2.7.2 Ultrasonic-microwave assisted extraction第46-47页
            2.2.7.3 Ultrasonic-homogenizer assisted extraction第47页
        2.2.8. Analysis of extracts activity第47-50页
            2.2.8.1.Determination of Total phenolic content第47页
            2.2.8.2.Evaluation of Antioxidant Activity第47-48页
            2.2.8.3.Designation of oxidative stability by Rancimat第48-49页
            2.2.8.4.Evaluation of Antibacterial Activity第49-50页
        2.2.9. Statistical analysis第50页
    2.3.Results and Discussions第50-62页
        2.3.1. Effect of extraction treatment on TPC第50-51页
        2.3.2. Anti-free radical activity第51-54页
        2.3.3. Thermal oxidation of RBDPOo第54-59页
            2.3.3.1.Without synergist of CA第54-56页
            2.3.3.2.With synergist of CA第56-57页
            2.3.3.3.The effect of CA synergist on oxidative stability of RBDPOosystems第57-59页
        2.3.4. Antibacterial Activity第59-62页
    2.4.Conclusion第62页
    2.5.References第62-67页
Chapter 3. Isolation of essential oil from Lavandula pubescens and assessment ofits biological activities第67-99页
    3.1. Introduction第67-68页
    3.2. Material and methods第68-75页
        3.2.1. Plant materials第68页
        3.2.2. Chemicals第68-69页
        3.2.3. Integration of ultrasonic-microwave equipment第69页
        3.2.4. Preliminary experiments of essential oil isolation第69-71页
            3.2.4.1. Isolation of Lp.EO based on enzymolysis pre-treatment第69-70页
            3.2.4.2. GC and GC-MS analysis第70页
            3.2.4.3. Scanning electron microscopy (SEM)第70-71页
            3.2.4.4. Free radical-scavenging activity of Lp.EO第71页
        3.2.5. Isolation of Lp.EO based on the ultrasound-microwave technique (US-MIC)第71-72页
        3.2.6. Experimental design and statistical analysis using Box-Behnken design(BBD)第72-74页
        3.2.7. Anti-bacterial activity by Agar well diffusion assay第74-75页
        3.2.8. Statistical analysis第75页
    3.3. Results and Discussions第75-92页
        3.3.1. Effect of enzymolysis pre-treatment on the yield% of Lp.EO第75-77页
        3.3.2. GC and GC-MS analysis of Lp.EO第77-80页
        3.3.3. Effect of pre-enzymatic pre-treatment on the morphological structuralof the Lp leaves第80-81页
        3.3.4. Free radical-scavenging assay第81-82页
        3.3.5. Effect of cavitation bubble collapse on the morphological structural andyield% of Lp.EO第82-83页
        3.3.6. Experimental design第83-89页
            3.3.6.1.Models Fitting第83-86页
            3.3.6.2.Yield%第86-88页
            3.3.6.3.DPPH·-SA%第88页
            3.3.6.4.ABTS·+-SA (mM Trolox)第88-89页
        3.3.7. Validation of the model第89-90页
        3.3.8. Anti-bacterial activity第90-92页
    3.4. Conclusion第92页
    3.5. References第92-99页
Chapter 4. Isolation of essential oil from Lavandula angustifolia and assessmentof its biological activities.第99-135页
    4.1. Introduction第99-100页
    4.2. Material and methods第100-108页
        4.2.1. Plant materials第100页
        4.2.2. Chemicals第100页
        4.2.3. Ultrasonic equipment第100-101页
        4.2.4. Single-factor experimental第101-103页
            4.2.4.1.Isolation and enzymolysis pre-treatment第101页
            4.2.4.2.Free radical-scavenging effect of La.EO第101-102页
            4.2.4.3.GC and GC-MS analysis第102-103页
            4.2.4.4.Scanning electron microscopy (SEM)第103页
            4.2.4.5.Isolation of the La.EO based on the ultrasonic-microwaveassisted第103页
        4.2.5. Orthogonal test design (BBD)第103-105页
        4.2.6. Anti-pathogenic bacterial activity第105-106页
            4.2.6.1.Pathogenic bacterial strain第105页
            4.2.6.2.Agar well diffusion第105-106页
        4.2.7. La.EO nanoparticles preparation第106-108页
            4.2.7.1.The granulometric profiles of La.EO nanoemulsions第106页
            4.2.7.2.Observation of confocal laser scanning microscopy (CLSM)第106-107页
            4.2.7.3.Transmission electron microscopy (TEM)第107页
            4.2.7.4.X-Ray Diffraction第107页
            4.2.7.5.Fourier-transform infrared spectroscopy (FTIR)第107-108页
            4.2.7.6.Morphological observation of SFo-La.EO-loaded WPInanoparticles第108页
            4.2.7.7.Investigation of WPI-SFo/La.EO bioactivity第108页
        4.2.8. Statistical analysis第108页
    4.3. Results and Discussions第108-129页
        4.3.1. Single factor experimental第108-115页
            4.3.1.1.Effect of enzymatic pre-treatment on the yield% of La.EO第108-111页
            4.3.1.2.GC and GC-MS analysis of La.EO第111-113页
            4.3.1.3.Morphological changes on the surface structure of the La(SEM)第113页
            4.3.1.4.Free radical-scavenging effect第113-115页
            4.3.1.5.Effect of the ultrasonic-microwave assisted on the yield% ofLa.EO第115页
        4.3.2. Optimization of La.EO yield% and free radical-scavenging effect usingBBD第115-120页
            4.3.2.1.Fitting the response surface models第116-117页
            4.3.2.2.Yield%第117页
            4.3.2.3.DPPH·-SA%第117页
            4.3.2.4.ABTS·+第117-120页
        4.3.3. Verification and validation of predictive model第120-121页
        4.3.4. Anti-pathogenic bacterial activity第121-122页
        4.3.5. The granulometric profiles and morphological characterizations ofLa.EO nanoparticles第122-126页
        4.3.6. XRD analysis第126-127页
        4.3.7. FTIR attributes of La.EO nanoparticles第127-129页
        4.3.8. Morphological observation of SFo-La.EO-loaded WPI nanoparticles第129页
    4.4. Conclusion第129-130页
    4.5. References第130-135页
Chapter 5. General Conclusions, Key Innovation and Recommendations第135-137页
    General conclusions第135页
    Key innovation of thesis第135-136页
    Recommendations第136-137页
List of Publications第137页

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