| 摘要 | 第9-11页 |
| ABSTRACT | 第11-12页 |
| List of abbreviations | 第13-16页 |
| Chapter 1 Introduction | 第16-42页 |
| 1.1. The Rabies virus and Rabies | 第16-27页 |
| 1.1.1. Taxonomy | 第17页 |
| 1.1.2. Virus structure and genome organization | 第17-19页 |
| 1.1.3. Viral gene expression and RNA synthesis | 第19-21页 |
| 1.1.4. Viral replication cycle | 第21-23页 |
| 1.1.5. Pathogenicity | 第23-24页 |
| 1.1.6. Mechanisms of RABV to counteract innate immunity | 第24-27页 |
| 1.2. Clinical Rabies | 第27-33页 |
| 1.2.1. Transmission of Rabies | 第27-28页 |
| 1.2.2. Movement of RABV towards brain | 第28-29页 |
| 1.2.3. Immunopathology | 第29-30页 |
| 1.2.4. Clinical disease symptoms | 第30-31页 |
| 1.2.5. Rabies Diagnosis | 第31-32页 |
| 1.2.6. Biosafety, sampling and specimen transport for laboratory Diagnosis | 第32-33页 |
| 1.2.7. Laboratory techniques for post-mortem diagnosis of rabies | 第33页 |
| 1.3. Current situation of rabies | 第33-35页 |
| 1.3.1. India | 第35页 |
| 1.3.2. P.R. China | 第35页 |
| 1.4. Rabies control program through immunization | 第35-39页 |
| 1.4.1. Traditionally in-use vaccines | 第36页 |
| 1.4.2. Cell culture based vaccines | 第36-37页 |
| 1.4.3. Modern Era in Vaccinology | 第37-39页 |
| 1.5. Use of Mononegavirales as viral vectors | 第39-40页 |
| 1.6. Conclusive aim of this study | 第40-42页 |
| Chapter 2 Materials and Methods | 第42-60页 |
| 2.1. Materials | 第42-49页 |
| 2.1.1. Cells, Viruses, Antibodies and Animals | 第42页 |
| 2.1.2. Plasmid Vector and Escherichia coli | 第42页 |
| 2.1.3. Reagents and Equipments | 第42-45页 |
| 2.1.3.1. Enzymes (Restriction and Ligation), buffers and stabilizing agent | 第42-43页 |
| 2.1.3.2. Kits used in viral recombination procedure | 第43页 |
| 2.1.3.3. Virus genome extraction and transfection reagents | 第43-44页 |
| 2.1.3.4. Plasmid Extraction (Alkaline lysis method) | 第44-45页 |
| 2.1.4. Escherichia coli culture media and related reagents | 第45-46页 |
| 2.1.5. SDS PAGE & Western Blot Material | 第46-47页 |
| 2.1.6. Cell culture working medium | 第47-48页 |
| 2.1.7. Apoptosis material provided with kit (Annexin V-FITC kit, e Bioscience) | 第48页 |
| 2.1.8. Sucrose Gradient material | 第48-49页 |
| 2.2. Methods | 第49-60页 |
| 2.2.1. The construction of LBNSE-GL-G and LBNSE-PM-G cDNA Clones | 第49-52页 |
| 2.2.2. Rescue of recombinant RABVs LBNSE-GL-G and LBNSE-PM-G | 第52页 |
| 2.2.3. Virus Titration | 第52-53页 |
| 2.2.4. Assay of Apoptosis | 第53页 |
| 2.2.5. Western Blot Analysis | 第53-57页 |
| 2.2.6. Virus Purification | 第57-58页 |
| 2.2.7. Immunization and Challenge Experiment | 第58-59页 |
| 2.2.8. Statistical Analysis | 第59-60页 |
| Chapter 3 Results and Analysis | 第60-74页 |
| 3.1. Generation and characterization of rRABVs expressing two copies of G protein | 第60-65页 |
| 3.1.1. The development of LBNSE-GL-G and LBNSE-PM-G c DNA clones | 第60-61页 |
| 3.1.2. Examination of rescued rRABVs (LBNSE-PM-G and LBNSE-GL-G) | 第61-62页 |
| 3.1.3. Growth curves of r RABVs on BSR and NA cells | 第62-63页 |
| 3.1.4. The mRNA expression of G-protein in recombinant RABVs | 第63-65页 |
| 3.2. G and M proteins produced in different rRABVs infected cells | 第65-66页 |
| 3.3. Apoptosis induced by different rRABVs | 第66-68页 |
| 3.4. G and M proteins incorporated in the rRABVs virons | 第68-69页 |
| 3.5. Immunogenicity of rRABVs (Live or Inactivated) in a mouse model | 第69-74页 |
| 3.5.1. Immunogenicity of rRABVs in a mouse model | 第69-71页 |
| 3.5.2. Immunogenicity through inactivated rRABVs in a mouse model | 第71-73页 |
| 3.5.3. The challenge experiment and survivorship analysis | 第73-74页 |
| Chapter 4 Discussion | 第74-78页 |
| Conclusion | 第78-79页 |
| References | 第79-94页 |
| List of Figures and Tables | 第94-96页 |
| Acknowledgement | 第96-98页 |
