| 摘要 | 第1-7页 |
| Abstract | 第7-13页 |
| Abbreviation | 第13-14页 |
| CHAPTER 1. INTRODUCTION | 第14-28页 |
| ·The honeybees | 第14页 |
| ·Origin and Taxonomy of Honeybees | 第14-15页 |
| ·Economic Importance of Honeybee | 第15-16页 |
| ·Honeybee Biology | 第16-20页 |
| ·Honeybee Casts and Division of labours | 第16-17页 |
| ·Basic Anatomy of Honeybees | 第17-18页 |
| ·The Nutrition of Honeybees | 第18页 |
| ·Developmental Stages of Honeybee | 第18-20页 |
| ·Honeybee Molecular Research | 第20-22页 |
| ·Significance of Honeybee Research | 第20-21页 |
| ·Challenges of honeybee Research | 第21页 |
| ·Honeybee Proteomic Research | 第21-22页 |
| ·Experimental Approach:Gel-based workflow of Proteomics | 第22-26页 |
| ·Protein Sample Preparation | 第23-24页 |
| ·Protein Separation by 2-DE | 第24页 |
| ·Detection and Visualisation of Proteins | 第24-25页 |
| ·Mass Spectrometry (MS) | 第25页 |
| ·Database Searching | 第25-26页 |
| ·Bioinformatics | 第26页 |
| ·Research Aims and Hypothesis | 第26-28页 |
| CHAPTER 2. PROTEOME ANALYSIS OF DIFFERENTIALLY EXPRESSED PROTEINS DURINGTHE EMBRYO-LARVA TRANSITION OF WORKER HONEYBEES (APIS MELLIFERA L.) | 第28-54页 |
| ·Summary | 第28-29页 |
| ·Introduction | 第29-30页 |
| ·Objective of the study | 第30页 |
| ·Material and methods | 第30-35页 |
| ·Chemical Reagents | 第30页 |
| ·Biological Sample Collection | 第30-31页 |
| ·Protein Sample Preparation | 第31页 |
| ·Two-Dimensional Gel Electrophoresis(2-DE) | 第31页 |
| ·Fluorescent Gel Staining | 第31-32页 |
| ·Image Analysis | 第32页 |
| ·Tryptic Digestion for MS | 第32-33页 |
| ·Mass Spectrometry and Protein Identification | 第33页 |
| ·Protein Classification | 第33-34页 |
| ·Biological Interaction Network and Functional Enrichment Analysis | 第34页 |
| ·est of Protein Expression by qRT-PCR | 第34-35页 |
| ·Results | 第35-49页 |
| ·Identification of Differentially Expressed Proteins | 第35-36页 |
| ·Qualitative Comparison of Differentially Expressed Proteins | 第36-43页 |
| ·Quantitative Comparisons of Differentially Expressed Proteins | 第43-44页 |
| ·Gene Ontology(GO)Functional Enrichment Analysis | 第44-45页 |
| ·Biological Interaction Network Analysis | 第45-46页 |
| ·Test of Differentially Expressed Proteins by qRT-PCR | 第46-49页 |
| ·Discussions | 第49-53页 |
| ·Conclusions | 第53-54页 |
| CHAPTER 3.IDENTIFICATION OF DIFFERENTIALLY PHOSPHORYLATEDPROTEINSDURING THE LAST DAY EMBRYO TRANSITION INTO THE FIRST INSTARLARVA OF WORKER HONEYBEES (APIS MELLIFERA L.) | 第54-70页 |
| ·Summary | 第54-55页 |
| ·Introduction | 第55-56页 |
| ·Objectives | 第56页 |
| ·Material and methods | 第56-58页 |
| ·Chemical reagents | 第56页 |
| ·Biological sample | 第56页 |
| ·Protein extraction and Two-Dimensional Gel electrophoresis | 第56-57页 |
| ·Image Acquisition and Statistical Analysis | 第57-58页 |
| ·Trypsin Digestion for MS and Proteins Identification by MS | 第58页 |
| ·Functional classification | 第58页 |
| ·Results | 第58-65页 |
| ·Identification of differentially phosphorylated proteins | 第58-59页 |
| ·Functional Classification | 第59-60页 |
| ·Qualitative and Quantitative Comparisons of Differentially Expressed Phosphoproteins | 第60-65页 |
| ·Discussions | 第65-68页 |
| ·Conclusions | 第68-70页 |
| CHAPTER 4 GENERAL CONCLUSION | 第70-71页 |
| REFERENCE | 第71-85页 |
| ACKNOWLEDGEMENTS | 第85-86页 |
| AUTHOR'S BIOGRAPHY | 第86-87页 |
