| Abstract | 第1-8页 |
| Abbreviations | 第8-10页 |
| Introduction | 第10-26页 |
| Materials | 第26-30页 |
| Enzymes | 第26页 |
| Chemicals | 第26页 |
| Plasmids | 第26-27页 |
| Apparatus | 第27页 |
| Medium | 第27页 |
| Buffers and other reagents | 第27-30页 |
| Methods | 第30-49页 |
| PCR | 第30-32页 |
| Restriction and recovery of PCR products | 第32-34页 |
| Ligation | 第34页 |
| Preparation of competent cell | 第34-35页 |
| Transformation | 第35页 |
| Check of recombinant colony | 第35-37页 |
| Expression check with Western Blot | 第37-39页 |
| Protein expression | 第39页 |
| Protein purification | 第39-40页 |
| Construction of expression system of human nNOSs and CaM | 第40页 |
| Protein expression and purification of human nNOSs and CaM | 第40-44页 |
| Spectrophotometric methods | 第44-47页 |
| Stopped-flow spectroscopy | 第47-49页 |
| Results | 第49-73页 |
| Construction of the expression system of human nNOS full-length enzyme | 第49-50页 |
| Expression and purification of human nNOS full-length enzyme | 第50-52页 |
| Purification of CaM and buman nNOS-s | 第52页 |
| Spectral characteristics of purified E.coli-Expressed enzyme | 第52-59页 |
| Reductase activities of E.coli-expressed human nNOS | 第59-63页 |
| Human neuronal nitric oxide synthase and its flavin domains catalyze one-electron reduction of Adriamycin, Mitomycin c, Menadione | 第63-66页 |
| The superoxide formation by Adriamycin radical | 第66-68页 |
| The flavin intermediates observed during the oxidation of the reduced enzymes by Adriamycin | 第68-73页 |
| Discussion | 第73-79页 |
| Reference | 第79-87页 |
| List of publication | 第87-88页 |
| Acknowledgments | 第88-89页 |
| Appendix | 第89-93页 |
| cDNA sequence of human nitric oxide synthase | 第89-92页 |
| Amino acid sequence of nitric oxide synthase | 第92-93页 |
| Plasmid map of pBluscript SKⅠ(-)/human nNOS | 第93页 |
| Plasmid map of pCWori+ | 第93页 |
