| 学位论文数据集 | 第4-5页 |
| ABSTRACT | 第5-7页 |
| 摘要 | 第8-14页 |
| Chapter 1 Introduction | 第14-40页 |
| 1.1 Bioremediation | 第14-16页 |
| 1.2 Algae and its role in bioremediation | 第16-18页 |
| 1.3 Heavy metal | 第18-19页 |
| 1.4 Sources and transport of heavy metals | 第19-20页 |
| 1.5 Heavy metals disadvantages to human, animal and environment | 第20-25页 |
| 1.5.1 Mercury effects | 第20-21页 |
| 1.5.2 CADMIUM and effects | 第21-22页 |
| 1.5.3 LEAD and their EFFECTS | 第22-23页 |
| 1.5.4 Copper and their effects | 第23页 |
| 1.5.5 Aluminum and its effects | 第23-24页 |
| 1.5.6 Iron and its effects | 第24-25页 |
| 1.6 Removal of mercury, cadmium, Copper, Iron, Aluminum and lead from water using living algae | 第25-26页 |
| 1.7 Removal of cadmium, lead, iron, copper, aluminum and mercury from soil using living algae | 第26-28页 |
| 1.8 Mechanism of heavy metals removal from water and soil | 第28页 |
| 1.9 Adsorption method | 第28-29页 |
| 1.10 Biosorption by algae | 第29页 |
| 1.11 Nanoparticles and their biological uses | 第29-30页 |
| 1.12 Types of Nanoparticles | 第30-35页 |
| 1.12.1 Engineered Nano-particles | 第30-31页 |
| 1.12.2 Non-Engineered Nano-particles | 第31页 |
| 1.12.3 Silver Nano-particles | 第31-32页 |
| 1.12.4 Gold Nano-particles | 第32-33页 |
| 1.12.5 Magnetic Nano-particles | 第33页 |
| 1.12.6 Synthesis of Nanoparticles | 第33-34页 |
| 1.12.7 Application of Nano-particles | 第34-35页 |
| 1.13 Nano-particle Applications in drug delivery | 第35-38页 |
| 1.13.1 Nano-particle Applications in food | 第35页 |
| 1.13.2 Nano-particle Applications in gene delivery | 第35页 |
| 1.13.3 Nano-particle Applications in the treatment of cancer | 第35-36页 |
| 1.13.4 Other applications of nanoparticle | 第36-38页 |
| 1.14 Objective | 第38-40页 |
| Chapter 2 Material and methodology | 第40-53页 |
| 2.1 Strains selection | 第40页 |
| 2.2 Cleaning of glassware | 第40页 |
| 2.3 Materials and reagents | 第40-41页 |
| 2.4 Collection of Soil samples | 第41页 |
| 2.5 The growth of Algae | 第41-42页 |
| 2.6 Screening of Algae | 第42页 |
| 2.7 Preparation of stock solutions of heavy metals | 第42页 |
| 2.8 Growth on Agar plates | 第42-43页 |
| 2.9 Removal | 第43页 |
| 2.10 Heavy metal removal from liquid medium | 第43-44页 |
| 2.11 Biosorption Experiments in soil | 第44页 |
| 2.12 Scanning electron microscopy (SEM) | 第44-45页 |
| 2.13 Leakage of Protein and Nucleic acid | 第45页 |
| 2.14 Confocal laser-scanning microscopy (CLSM) | 第45-46页 |
| 2.15 Materials | 第46页 |
| 2.16 Cultivation of Algae | 第46-47页 |
| 2.16.1 Preparation of Algal extract | 第46页 |
| 2.16.2 Synthesis of gold and silver nanoparticle | 第46-47页 |
| 2.17 The redox potential of JSC-1 extracts | 第47页 |
| 2.18 Characterization of Nanoparticles | 第47-48页 |
| 2.18.1 UV-Visible spectroscopy | 第47页 |
| 2.18.2 Transmission electron microscopy (TEM) | 第47-48页 |
| 2.18.3 Scanning electron microscopy and Energy dispersive X-ray (SEM and EDX) | 第48页 |
| 2.18.4 X-ray diffraction (XRD) | 第48页 |
| 2.18.5 Fourier transformed infrared (FTIR) | 第48页 |
| 2.19 DLS and Zeta potential | 第48-50页 |
| 2.19.1 The activity of Au NPs | 第49-50页 |
| 2.19.1.1 Photo-catalytic activity | 第49页 |
| 2.19.1.2 Antibacterial activity | 第49页 |
| 2.19.1.3 Investigation of reactive oxygen species (ROS) | 第49-50页 |
| 2.19.1.4 Membrane disruption | 第50页 |
| 2.20 The activity of Ag NPs | 第50-53页 |
| 2.20.1 Growth inhibition study Bacterial growth curve | 第50-51页 |
| 2.20.2 Well diffusion method | 第51页 |
| 2.20.3 Cell damaged | 第51页 |
| 2.20.4 Anticancer Activity | 第51-52页 |
| 2.20.5 In Vitro Cell Uptake of Ag NPs | 第52-53页 |
| CHAPTER 3 Results and discussion of Bioremediation of Hg, Pb and Al | 第53-78页 |
| 3.1 Physical and chemical properties | 第53-56页 |
| 3.2 Twenty-two strains growth on minute concentration | 第56-57页 |
| 3.3 Selected algae growth with different concentration | 第57-62页 |
| 3.3.1 Leakage of protein | 第60-62页 |
| 3.4 Nucleic acid leakage | 第62-70页 |
| 3.4.1 Removal with different concentration | 第64-67页 |
| 3.4.2 Removal From water | 第67-70页 |
| 3.5 Heavy metals removal from Soil | 第70-72页 |
| 3.6 Scanning Electron Microscopy | 第72-75页 |
| 3.7 Confocal Study | 第75-78页 |
| Chapter 4 Results and discussion Bioremediation of Cd, Cu and Fe | 第78-100页 |
| 4.1 Twenty-two strains growth on minute concentration | 第79-80页 |
| 4.2 Selected algae growth with different concentration | 第80-83页 |
| 4.3 Leakage of protein | 第83-85页 |
| 4.4 Nucleic acid leakage | 第85-93页 |
| 4.4.1 Removal with different concentration | 第87-90页 |
| 4.4.2 Removal From water | 第90-93页 |
| 4.5 Heavy metals removal from Soil | 第93-95页 |
| 4.6 Scanning Electron Microscopy | 第95-98页 |
| 4.7 Confocal Study | 第98-100页 |
| CHAPTER 5 Results and discussion synthesis of gold-silvernanoparticles | 第100-114页 |
| 5.1 Redox potential for the mixture of HAuCl_4 solution and JSC-1 extracts | 第100-101页 |
| 5.2 Characterization of Nan particles | 第101-114页 |
| 5.2.1 UV analysis | 第101-102页 |
| 5.2.2 TEM and SEM analysis of gold nanoparticles | 第102-103页 |
| 5.2.3 EDX | 第103-104页 |
| 5.2.4 XRD analysis | 第104-105页 |
| 5.2.5 FTIR study | 第105-107页 |
| 5.2.6 Photo-catalytic activity | 第107-108页 |
| 5.2.7 Antibacterial activity | 第108-109页 |
| 5.2.8 Determination of ROS | 第109-110页 |
| 5.2.9 Membrane disruption | 第110-114页 |
| CHAPTER 6 Results and discussion of Ag NPs and their properties | 第114-128页 |
| 6.1 Synthesis of Ag NPs and UV-visible spectrometry | 第114-116页 |
| 6.2 TEM analysis of Ag NPs | 第116-117页 |
| 6.3 Scanning electron microscopy, (SEM) and energy dispersive x-ray spectroscopy, (EDX) | 第117页 |
| 6.4 XRD and FTIR analysis | 第117-118页 |
| 6.5 Fourier transforms infrared spectroscopy (FTIR) | 第118-119页 |
| 6.6 Dynamic light scattering, (DLS) and ZETA Potential Distribution | 第119-120页 |
| 6.7 Inhibition of bacterial growth and antibacterial activity | 第120-122页 |
| 6.8 Cell membrane disruption | 第122-123页 |
| 6.9 Anticancer Activity using HeLa cell line | 第123-125页 |
| 6.10 Analysis of Apoptosis | 第125-126页 |
| 6.11 Cellular uptake of AgNPs by HeLa cell line | 第126-128页 |
| Chapter 7 Conclusion | 第128-130页 |
| References | 第130-139页 |
| Future Plan | 第139-140页 |
| Acknowledgements | 第140-143页 |
| Resume of Author | 第143-146页 |
| Resume of Supervisor | 第146-151页 |
| List of Publications | 第151-152页 |
| 附件1 | 第152-154页 |
| 附件2 | 第154-155页 |
