| Abstract | 第4-5页 |
| 摘要 | 第6-11页 |
| Chapter 1 Introduction and literature review | 第11-34页 |
| 1.1 ENZYME DEFINITION AND ROLE | 第11-12页 |
| 1.2 MICHAELIS-MENTEN KINETIC | 第12-14页 |
| 1.3 ENZYME INHIBITION | 第14-19页 |
| 1.3.1 Inhibition types | 第14-18页 |
| 1.3.2 IC_(50) and K_i | 第18-19页 |
| 1.4 ENZYME ASSAY | 第19-29页 |
| 1.4.1 Enzyme assay based CE | 第19-29页 |
| 1.5 RESEARCH OBJECTIVE | 第29-30页 |
| References | 第30-34页 |
| Chapter 2 Application of Capillary Enzyme Micro reactor in Enzyme Activity andInhibitors Studies of Glucose-6-phosphate Dehydrogenase | 第34-51页 |
| 2.1 INTRODUCTION | 第34-36页 |
| 2.2 MATERIALS AND METHODS | 第36-38页 |
| 2.2.1 Chemicals | 第36页 |
| 2.2.2 Fabrication of CE-based G6PDH-IMERs | 第36-37页 |
| 2.2.3 Online Enzyme and inhibition Assay | 第37-38页 |
| 2.2.4 Offline enzyme and inhibition assay | 第38页 |
| 2.3 RESULTS AND DISCUSSION | 第38-47页 |
| 2.3.1 Optimization of the CE-based G6PDH-IMERs | 第38-40页 |
| 2.3.2 Repeatability and stability of the CE-based G6PDH-MERs | 第40-42页 |
| 2.3.3 Enzyme/inhibition assay using the CE-based G6PDH-IMERs | 第42-47页 |
| 2.4 CONCLUSION | 第47-49页 |
| References | 第49-51页 |
| Chapter 3 Determination of the inhibitory effect of green tea extract on glucose-6-phosphate dehydrogenase based on multi-layer capillary enzyme micro reactor | 第51-65页 |
| 3.1 INTRODUCTION | 第51-53页 |
| 3.2 EXPERIMENTAL | 第53-55页 |
| 3.2.1 Chemicals and instruments | 第53-54页 |
| 3.2.2 Online enzyme and inhibition assay of G6PDH using multi-layer CE-IMERs | 第54页 |
| 3.2.3 Offline enzyme and inhibition assay | 第54-55页 |
| 3.3 RESULTS AND DISCUSSION | 第55-62页 |
| 3.3.1 Performance of the multi-layer CE-IMERs | 第55-58页 |
| 3.3.2 Enzyme/inhibition assay using the two-layer CE-IMERs | 第58-62页 |
| 3.4 CONCLUSION | 第62-63页 |
| References | 第63-65页 |
| Chapter 4 Development of brush-modified immobilized open tubular capillary enzymemicro reactor for efficient protein digestion | 第65-78页 |
| 4.1 INTRODUCTION | 第65-66页 |
| 4.2 MATERIALS AND METHODS | 第66-69页 |
| 4.2.1 Chemicals and materials | 第66-67页 |
| 4.2.2 IMER fabrication and characterization | 第67-68页 |
| 4.2.3 Enzyme assay using IMERs | 第68页 |
| 4.2.4 Digestion of proteins by trypsin IMER | 第68-69页 |
| 4.2.5 CE conditions for proteins hydrolysat | 第69页 |
| 4.3 RESULTS AND DISCUSSION | 第69-75页 |
| 4.3.1 Characterizations of silica brush capillary | 第69-70页 |
| 4.3.2 Kinetic characterization of the immobilized trypsin | 第70-71页 |
| 4.3.3 Reuse and storage stability of the immobilized trypsin | 第71-73页 |
| 4.3.4 Protein analysis | 第73-75页 |
| 4.4 CONCLUSION | 第75-76页 |
| References | 第76-78页 |
| Chapter 5 Application of TLC for investigation of enzyme activities and its inhibition bydensitometric detection of NADH based on enzymatic reaction | 第78-86页 |
| 5.1 INTRODUCTION | 第78-79页 |
| 5.2 EXPERIMENT | 第79-81页 |
| 5.2.1 Materials | 第79页 |
| 5.2.2 Principle of the TLC G6PDH Assay | 第79-80页 |
| 5.2.3 Offline Enzyme Assay | 第80-81页 |
| 5.3 RESULTS AND DISCUSSION | 第81-84页 |
| 5.3.1 Optimization of the On-Plate Enzyme Reaction | 第81-82页 |
| 5.3.2 On-plate enzyme assay and inhibition study | 第82-84页 |
| 5.4 CONCLUSION | 第84-85页 |
| References | 第85-86页 |
| Chapter 6 Conclusions and future directions | 第86-88页 |
| 6.1 CONCLUSIONS | 第86-87页 |
| 6.2 FUTURE DIRECTIONS | 第87-88页 |
| Acknowledgements | 第88-90页 |
| 在读期间公开发表论文情况 | 第90-91页 |
